Transfection of Airway Epithelium by Stable PEGylated Poly-L-lysine

ARTICLE

doi:10.1016/j.ymthe.2003.07.007

Transfection of Airway Epithelium by Stable PEGylated Poly-L-lysine DNA Nanoparticles in Vivo Assem-Galal Ziady,1,* Christopher R. Gedeon,2 Timothy Miller,2 William Quan,1 Jennifer M. Payne,2 Susannah L. Hyatt,2 Tamara L. Fink,2 Osman Muhammad,2 Sharon Oette,2 Tomasz Kowalczyk,2 Murali K. Pasumarthy,2 Robert C. Moen,2 Mark J. Cooper,2 and Pamela B. Davis1 1

Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio 44106 2 Copernicus Therapeutics, Inc., Cleveland, Ohio 44106

*To whom correspondence and reprint requests should be addressed at the Department of Pediatrics, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106-6006. Fax: (216) 368-4223. E-mail: [email protected].

DNA can be compacted using polyethylene glycol-substituted poly-L-lysine into discrete unimolecular (with respect to DNA) nanoparticles with minor diameter