Two Bone Alkaline Phosphatase Assays ... - Semantic Scholar
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Two Bone Alkaline Phosphatase Assays ... - Semantic Scholar
Apr 11, 1994 - Serum bone alkaline phosphatase (ALP; EC 3.1.3.1) was measured with a .... mm at 65#{176}C)in these sera, the residual placental. ALP.
CLIN.CHEM.41/2, 196-199 (1995)
#{149} Enzymes
and Protein
Markers
Two Bone Alkaline Phosphatase Assays Compared with Osteocalcin as a Marker of Bone Formation in Healthy Elderly Women Anna
A. Bouman,”4
Peter G. Scheffer,’
Marcel
E. Ooms,2 Paul Lips,3 and Coen
Serum bone alkaline phosphatase (ALP; EC 3.1.3.1) was measured with a wheat germ agglutinin (WGA) precipitation assay and with a new IRMA in a group of healthy elderly women. Both assays were correlated with serum total ALP activity and with osteocalcin. The two bone ALP assays have comparable within- and between-run imprecisions (WGA assay within-run CVs 2.6-5.4% and between-run, 4.0-5.1%; IRMA within-run CV 5.0% and between-run, 3.2%). Comparison of the WGA precipitation assay (x) with the IRMA (y) demonstrated a correlation coefficient of 0.87 [Deming regression equation: y = (0.58 ± 0.02)x (4.62 ± 0.45); n = 101; S = 1.26; P
