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subjected to four acclimation conditions (10°C or 20°C combined with ... (A) Control carp (x 100); acclimation to 20°C and (B) a photoperiod of 16L-8D (x 75) and (C) of ... wash with 0.5 x SSC at 37°C and, lastly, by a 30 min rinse with 0.5 x.