Int. J. Biol. Sci. 2012, 8
Ivyspring
International Publisher
1408
International Journal of Biological Sciences
Research Paper
2012; 8(10):1408-1417. doi: 10.7150/ijbs.4597
Up-regulated miR-145 Expression Inhibits Porcine Preadipocytes Differentiation by Targeting IRS1 Yunxue Guo1, Yaosheng Chen1,2, Yun Zhang1, Yue Zhang1, Luxi Chen1, Delin Mo1,2 1. 2.
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006 P. R. China; Guangdong Province Engineering and technology R&D centers for pig breeding, Sun Yat-sen University, Guangzhou, Guangdong 510006 P. R. China.
Corresponding author: Delin Mo, Tel: +86 (0)20 39332788; Fax: +86(0)20 39332940; E-mail:
[email protected]. © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/ licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
Received: 2012.05.16; Accepted: 2012.09.12; Published: 2012.11.12
Abstract Generally, most miRNAs that were up-regulated during differentiation promoted adipogenesis, but our research indicated that up-regulation of miR-145 in porcine preadipocytes did not promote but inhibit adipogenesis. In this study, miR-145 was significantly up-regulated during porcine dedifferentiated fat (DFAT) cells differentiation. In miR-145 overexpressed DFAT cells, adipogenesis was inhibited and triglycerides accumulation was decreased after hormone stimulation (P
