A. Elliyanti et al. / Atom Indonesia Vol. 42 No. 3 (2016) 145 - 149. Uptake and Cytotoxicity Characterization of. Radioiodine in MCF-7 and SKBR3 Breast Cancer.
Atom Indonesia Vol. 42 No. 3 (2016) 145 - 149
A. Elliyanti et al. / Atom Indonesia Vol. 42 No. 3 (2016) 145 - 149
Atom Indonesia Journal homepage: http://aij.batan.go.id
Uptake and Cytotoxicity Characterization of Radioiodine in MCF-7 and SKBR3 Breast Cancer Cell Lines A. Elliyanti1*, V.Y. Susilo2, S. Setiyowati2, M. Ramli2, J.S. Masjhur3 and T.H. Achmad4 1
Medical Physics/Radiology Department of Faculty of Medicine, Andalas University/Dr.M.Djamil Hospital Jl. Perintis Kemerdekaan No. 94, Padang 25127, Indonesia 2 Center for Radioisotopes and Radiopharmaceutical Technology, National Nuclear Energy Agency, Puspiptek Area, Serpong, Tangerang Selatan 15314, Indonesia 3 Department of Nuclear Medicine, Padjadjaran University, Bandung, Indonesia 4 Department of Biochemistry, Padjadjaran University, Bandung, Indonesia
ARTICLE INFO
ABSTRACT
Article history: Received 4 October 2015 Received in revised form 14 August 2016 Accepted 2 September 2016
Radioiodine is an effective and low-risk therapy modality in well-differentiated thyroid cancer patients post near-total thyroidectomy. Extra thyroidal tumors such as breast cancer are known to be able to uptake radioiodine. The aim of this study was to analyze the uptake, efflux and cytotoxicity of radioiodine for two molecular types of breast cancer cell lines. Two types of breast cancer cell lines were used in this study, MCF-7 (luminal A type) and SKBR3 (HER2 type). The HaCaT cell line was used as normal cells. Iodine-125 (I-125) was used to measured radioiodine uptake and efflux. Clonogenic assay was used to assess cytotoxicity of iodine-131 (I-131) based on the tested cell reproductive ability. The radioiodine uptake in SKBR3 cells was found to be higher than that of MCF-7 and HaCaT cells at p
