Vascular plants facilitated bryophytes in a grassland experiment

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In grassland communities vascular plants and bryophytes form two distinct layers. ... experiment one bryophyte species and one vascular plant species were ...
 Springer 2005

Plant Ecology (2005) 180:69–75 DOI 10.1007/s11258-005-2508-0

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Vascular plants facilitated bryophytes in a grassland experiment Nele Ingerpuu*, Jaan Liira and Meelis Pa¨rtel Institute of Botany and Ecology, University of Tartu, Lai 40, Tartu 51005, Estonia; *Author for correspondence (e-mail: [email protected]; fax: +3727 376222; phone: +372-737-6258) Received 29 June 2004; accepted in revised form 18 February 2005

Key words: Cover, density, ecology, moss, shading

Abstract In grassland communities vascular plants and bryophytes form two distinct layers. In order to understand the factors responsible for plant community structure, more information about interactions between these plant groups is needed. Often negative correlations between vascular plant and bryophyte covers have been reported, suggesting competition. Here we tested experimentally whether different grassland vascular plant species (Trifolium pratense, Festuca pratensis, Prunella vulgaris) had different influences on the cover of two bryophyte species (Rhytidiadelphus squarrosus, Brachythecium rutabulum). In a two-year garden pot experiment one bryophyte species and one vascular plant species were planted per pot. Bryophytes were planted at a constant density, vascular plants in four densities. The cover of both bryophyte species increased with increasing vascular plant cover, showing the facilitative effect of vascular plants through creating better microclimate, e.g., optimising temperature. Bryophyte responses to vascular plant species were species-specific. Festuca had significantly positive effects on both bryophyte species in the second year, and Trifolium on Brachythecium in both years, whereas Prunella had no significant effect on bryophytes. The facilitative effect of vascular plants was stronger at the second experimental year. In summary, the biotic effects between bryophytes and grassland vascular plants are species-specific and positive interactions are prevailing at low vascular plant densities.

Introduction Plant communities are driven by both large-scale processes – the evolutionary-historical effects forming species pools (Ricklefs 1987; Zobel 1992; Pa¨rtel et al. 1996), and small-scale processes – biotic interactions within communities (Tilman and Pacala 1993; Brown et al. 2001). The relative importance of these aspects depends on the abiotic conditions, e.g. the species pool effect is more important in oligotrophic habitats and, local processes are more important at low or high productivity (Huston 1999; Pa¨rtel et al. 2000; Foster 2001).

Biotic interactions include both negative and positive effects, which often act simultaneously (Callaway and Walker 1997; Brooker and Callaghan 1998; Pugnaire and Luque 2001). The role of competition has been emphasised in several species coexistence theories, but more recently facilitation has been found to be important (Callaway 1995; Stachowicz 2001; Callaway et al. 2002; Garcı´ a and Obeso 2003; Rudgers and Maron 2003). Biotic interactions are important for determining plant community structure, e.g., presence of distinct layers. For example, tree establishment in prairies is facilitated by the shrub layer (Li and Wilson 1998), and woody species are excluded from

70 prairies through intense root competition from grasses (Pa¨rtel and Wilson 2002). Compared to interactions between tree, shrub and field layers, bryophyte relations with vascular plants have been studied less. Most of these studies consider bryophytes on the forest floor. For example, bryophyte species composition differed between coniferous and hardwood forests in Northern America (Rambo and Muir 1998), and epilithic bryophyte composition in Scandinavia differed under a variety of tree species (Weibull 2001), bryophyte species richness was positively correlated with the number of tree species in deciduous forests (Ingerpuu et al. 2003). In contrast, under predominantly coniferous forests, no correlation between tree and bryophyte species compositions has been found (McCune and Antos 1981; Pharo and Vitt 2000), although a change in bryophyte composition is demonstrated from below to between trees in boreal forests (Økland and Eilertsen 1993). Due to large differences in size, trees affect bryophytes mostly through the coarse-scale ‘engineering’ of abiotic conditions: light, temperature, moisture, nutrient status and other chemical properties of substrates (Zobel et al. 1996; Jones et al. 1997). Bryophytes also form a distinct layer in grassland communities under herbaceous vascular plants. There are very few investigations where the effects of established herbaceous vascular plants on bryophytes have been studied experimentally (Levine 1999). Bryophyte species composition is related to the vascular plant species composition in some temperate grasslands (During and Lloret 1996; Zamfir et al. 1999; Ejrnæs and Poulsen 2001), but no relationships have been found in other locations (Herben 1987). Biomass of bryophytes and vascular plants, however, has been found to be negatively correlated in most studies. Often this has been explained by competition between these two layers (Ingerpuu et al. 1998; Zamfir et al. 1999; Virtanen et al. 2000; Bergamini et al. 2001). Bryophyte influence on herbaceous vascular plant germination and juvenile survival is mostly negative (Hein 1966; Ho¨rnberg et al. 1997; Zamfir 2000). In grassland communities, most of the vascular plants are perennials (Eriksson and Jakobsson 1998) and regeneration from seeds is common only after disturbances (Foster 2001). Bryophytes, however, have usually almost no

biotic effects on mature vascular plants (During and van Tooren 1990), which means that the bryophyte layer in grassland communities may be influenced by vascular plants through their biotic effect. Established herbaceous vascular plants can theoretically influence bryophytes both negatively and positively. A negative effect is the reduction of light availability. Grassland bryophytes are known to need relatively high light levels (Rincon and Grime 1989). In an Estonian wooded meadow, bryophytes were only common under a sparse upper layer (Kull et al. 1995). Another negative effect is competition for space. Perennial vascular plants can easily fill most of the space and bryophytes can only use the remaining space. Positive effects include protection from drought and the provision of nutrients captured through the symbiosis with nitrogen-fixing bacteria, or captured from the air (Callaway 1995). Biotic interactions may be species unspecific, but also species-specific (Callaway 1998). Species-specific architecture might either facilitate or suppress other species by enhancing or reducing space and light availability and species-specific chemical compounds can affect the growth of other species. To detect species specificity, experimental work with selected species pairs are needed. Our aim was to study how three grassland vascular plant species affect two bryophyte species in a two-year garden experiment: testing whether vascular plant cover has general or species-specific effects on bryophyte cover, and how does this relationship vary over time.

Materials and methods We used two bryophyte species common in European grasslands (Brachythecium rutabulum (Hedw.) B.S.G. and Rhytidiadelphus squarrosus (Hedw.) Warnst.), and three widespread vascular plant species (a legume – Trifolium pratense L., a grass – Festuca pratensis Huds. and a forb – Prunella vulgaris L.) in a garden pot experiment where a bryophyte species was grown under different densities of a vascular plant species for two years (2000–2001). Hereafter species are referred to by genus. The bryophytes were collected from a natural grassland 30 km south of the experiment garden,

71 air dried, and cut into pieces of 1–2 cm. A constant mass of bryophytes (0.2 g) was planted evenly into pots (21 · 32 cm, 8 cm deep) filled with a low fertility soil (mixture of sieved natural grassland soil and sand), one species per pot. A well-developed bryophyte carpet establishes from planted vegetative parts (Mulder et al. 2001). The pots were planted with the vascular plants, one vascular plant species per pot. The vascular plants were grown from seeds in a greenhouse and planted evenly as 2-weeks old juveniles in 4 densities: 3, 6, 12 and 24 plants in a pot, in order to obtain different degrees of cover. We used five replicates for each combination (two bryophyte species, and three vascular plant species at four densities). At the beginning of the experiment in May 2000, the cover of bryophytes was 3% and that of vascular plants was 0.5–12%. The pots were kept outside, on the ground in half-shade in a garden, located at the University of Tartu (5822¢ N, 2644¢ E). The pots were displaced randomly several times during the summers, watered during longer dry periods and weeded regularly. At the end of the growing seasons the covers of bryophytes and vascular plants were determined in percentages, the vascular plants were cut at a height of 1–2 cm, and the litter was removed to simulate grassland management. Temperature and photosynthetic active radiation was measured just above the bryophytes in the experimental pots parallel to the nearby dense moss layer (consisting mainly of Brachythecium rutabulum) without vascular plant cover in the end of the second growing season, on a sunny midday in August. The intensity of photosynthetic radiation was measured using a LI-191SA Line Quantum Sensor. The t-Test for dependent samples tested for difference in temperature or radiation with and without vascular plants. General Linear Model (GLM) tested for relationship between temperature or radiation reduction and vascular plant species and its cover. In order to analyse the effect of vascular plants on bryophyte species coverage, the repeated design was used in a General Linear Mixed Model (GLMM, proc mixed, SAS Institute 1992). The plot was defined as a subject, observed repeatedly over two years. Linear regression slopes were calculated between bryophyte and vascular plant cover for each bryophyte species, vascular plant

species, and observation year combination. Model residual distribution normality and homoscedasity was checked. A model with non-linear relationship (including the second-order term) was also tested, but it was omitted because of non-significant results.

Results The temperature and radiation under vascular plant cover in experimental pots were significantly lower than in control without vascular plants – mean difference in temperature 3.5 C (t-test for dependent samples: t = 14.5, df = 115, p