Mar 24, 1972 - ELI[CANAANI AND PETER DUESBERG. Department of Molecular Biology and Virus Laboratory, University of California, Berkeley, California ...
Role of Subunits of 60 to 70S Avian Tumor Virus Ribonucleic Acid in Its Template Activity for the Viral Deoxyribonucleic Acid Polymerase ELI[CANAANI AND PETER DUESBERG Department of Molecular Biology and Virus Laboratory, University of California, Berkeley, California 94720 Received for publication 24 March 1972
Heating the 60 to 70S ribonucleic acid (RNA) of Rous sarcoma virus (RSV) destroys both its subunit structure and its high template activity for RSV deoxyribonucleic acid (DNA) polymerase. In comparative analyses, it was found that the template activity of the RNA has a thermal transition of 70 C, whereas the 60 to 70S structure dissociates into 30 to 40S and several distinct small subunits with a Tm of 55 C. Analysis by velocity sedimentation and isopycnic centrifugation of the primary DNA product obtained by incubation of 60 to 70S RSV RNA with RSV DNA polymerase indicated that most, but perhaps not all, DNA was linked to small (
