X Chromosome Dosage Influences DNA Methylation Dynamics during ...

Stem Cell Reports, Volume 10

Supplemental Information

X Chromosome Dosage Influences DNA Methylation Dynamics during Reprogramming to Mouse iPSCs Vincent Pasque, Rahul Karnik, Constantinos Chronis, Paula Petrella, Justin Langerman, Giancarlo Bonora, Juan Song, Lotte Vanheer, Anupama Sadhu Dimashkie, Alexander Meissner, and Kathrin Plath

Female XO iPSCs

4 4 17 Female XaXa iPSCs

8 8 11 2

Female iPSC line 8

Female iPSC line 7

Female iPSC line 9

Female iPSC line 10

Female iPSC line 6

Female iPSC line 5

Passage number: Female iPSC line 4

% biallelic Tsix

Female iPSC line 3

Figure S1

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passage 13

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D Imprint control regions (CpGs covered in all samples) Methylation level

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Male SSEA1-

50% 25% 0% 100% 75%

Male SSEA1+

50% 25% 0% 100% 75%

Male iPSCs

50% 25% 0% 100% 75%

Male ESCs

50% 25% 0% 100% 75%

Female MEFs

50% 25% 0% 100% 75%

Female SSEA1-

50% 25% 0% 100% 75%

Female SSEA1+

50% 25% 0% 100% 75%

Female XaXa iPSCs

50% 25% 0% 100% 75%

Female XaO iPSCs

50% 25% 0% 100% 75%

Female XaXa ESCs

50% 25% 0%

0. 5

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