yii annual conference

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YII ANNUAL CONFERENCE DECEMBER zOth

TO 22I1d, 1980

KASTU RBA M ED ICAL COLLEGE MANIPAL r'

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group were L-glutamine, L-arginine, L-cysteine and dipeptides, glycylalanine and leucylalanine for both the variants of the enzyme. Oxidized glutathione at 4mM concentration'showed about B0o/" inhibition of enzyme activities of both forms. L-serine in the presence of sodium borate inhibited high molecular weight (Ki value:0.167mM) as well as the low molecular weight forms (Ki:0.069mM). The energy of activation fol the two variants of the enzyme was found to be almost sarne. The hydrolytic activity of high molecular weight variant was lower (K.: l9.04mM) than the low molecular weight variant

(K-:9.l9mM).

Q) UTILNY OF CERTNN CSF ENZYME ASSAYS IN

THE DIAGNOSIS OF

CIIYS DISORDERTI

l[. N. SITBIIASE, Y. R BHAGWAT, N. JAYASIMIIA and B. S. SRIDIIARA RAMA RAO, Neumchemistry Department, National Institute of Mental Healtb aad Neuro Scienct+ BAI.IGALOR.E - 560 029.

An analpis wzrs made of a battery of enzymes in CSF samples obtained from patients with different neuropsychiatric disorders. Spectrophotomeffic methods were used for the following enzymes - lactic dehydrogenase (LDH), malic dehydrogenase ( MDH ), phosphohexoseisomerase ( PHI ), transaminase (OT & PT), creatine phosphokinase (CPK) and aldolase (ALD). In fluids obtained from patients with CNS infections, LDH and PHI were markedly increased which showed a significant reduction after suitable therapy and remission. The changes in OT and PT paralleled somewhat the changes in LDH and PHI. A significant increase in LDH, PHI and CPK was observed in cases with brain tremors. The utility of CSF enzyme assay in diagnosis of CNS disorders

9.

will be discussed.

ISOENZYME PATTERNS AIYD DIAGNOSTIC IIYDI@S PROIYE TO MYOC:ARDIAL II{FARCTION

IN CASES

J. CfiAKRAVORTY, B. DAS and D. P. BURMA, Ocpartneat of Biochemisey, Institute of Medical Baaaras

It

Sgiences'

Hiadu University, VARAI\fASI - 221 005.

is well known that the level of LDH1 isoenzyme is approxrmately half of the LDH2 isoenzyme in the sera of normal healthy individuals. The LDH1/LDH2 ratio becomes more than one and sometimes even two (flipped LDH) in cases of myocardial infarction. Similarln CPK level is known to increase significantly and the MB isoen4rme band appeaxs in the serum within a short period following infarction. In our laboratory attention is being focussed more on cases of angina pectoris, old infarcts, hypertension etc., to find out whether the isoenzym.e patterns could be of any diagnostic value in cases Prone to infarcdon. ft has been obServed that some of the patients who have LDH1/LDH2 ratio more than 0.5 but less than unity (disturbed LDH pattern)

with matufe values and none of the IB subjects between 3643 weeks gestation was associated with immature values for these 4 lipid parameters. None of the 3 infants who developed respiratory distress syndrome was associated with mature values. Though levels of TL, TC, TPL and L as well as L/S ratios were higher in preeclampsia compared to normal pregnancy, the difference was not statistically significant. It is suggested that TPL content of AF above '6 mg/dl is a good index of foetatr lung maturity. The time-consuming L/S ratio procedure may be necessary only when TPL is in the transitional range of 3-6 mg/dl. 30 weeks gestation was associated

99.

POLYA]\,IINES

IN THE DIAGNOSIS OF GASTRIC CANCER

R. KAI.AVATIfY and G. SULOCHANA, Department of Thanjavur Medical College, THANJAVUR - 613 003.

Biochemistry

'

simple method for the detection and estimation of polyamines in gastric aspirate is described. After hydrolysis and extraction, the polyamines were separated by paper chromatography. The method was found to be useful as a guide in the early diagnosis of gastric cancer. Thirty cases of gastric cancer, both in the early and late stages of the disease, were screened by this method and the polyamines patterhs obtained, agreed well with histopathology reports. Normal gastric aspirates revealed no polyamine patterns by the chromatographic

A

method.

(tofi A coMpARATrvE ASSESSMENT oF DTFFERENT AIIALYTTCAL \-/ METHoDS usED FoR ESTIMATToN oF PRorErN IN csF V: R. IIIAGIIIAT, M. N. SUBHASH, N. JAYASIMHA and B. S. SRIDIIARA RAMA RAO,

Nerr-q6"-i#tD"partment, National Institute of Mental Health and Neuro

Sciences,

BANGALORE - 560 029'

o/o The protein content of CSF under normal circumstances is less than 50 mg and hence the method employed must be fairly sensitive. The present studies report observations made during the use of three different techniques simultaneorily'for the assay of protein in 250 patients with different neuropsychiatric disorders. The comparaave assessment was made of the turbidimetric methods (using trichloroacetic acid and perchloric acid), the colorimetric method (using Folin's reagent) and the dye binding method (using Ooorriassie Brilliant tslue). ft was found that the turbidimetric methods are unreliable unless optimal temperature is maintained. The coiorimetric method was found to be affected by medication. The present studies indicate that the dye binding method is sensitive and reproducible for different ranges of protein values and can be used routinely for the determination of proteins in CSF.

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ru ANNUAT IONFERENIE ASSOCIATIO]I OF CLITICAI BIOCHEMISTS OF IilDIA December 20-22, 1980 KA9TURBA MEDICAL COLLEGE MANIPAL 576 1 1 9, KARNATAKA

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Date: 22- 12-1980

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