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2. Appendix Table S1. X-ray data collection and refinement statistics. Dataset. Ctf18C-Dcc1-Ctf8. Dcc190-380 Native. Dcc190-380 Peak. Dcc190-380 Inflection.

APPENDIX

Contents Appendix Table S1. X-ray data collection and refinement statistics

1

Appendix Table S1. X-ray data collection and refinement statistics 90-380

Dcc1

Wavelength (Å)

0.9763

0.92

0.9796

0.9797

Resolution range

60.64 - 2.29 (2.372 2.29) P21

29 - 2.002 (2.074 2.002) P21

79.19 - 2.75 (2.82 2.75) P212121

112.75 - 2.96 (3.04 2.96) P212121

Total reflections

58.61 164.22 60.64 90 90.55 90 138884 (5577)

68.682 100.697 83.495 90 100.67 90 288441 (23107)

96.69 111.75 112.23 90.00 90.00 90.00 415363 (28991)

96.82 112.02 112.75 90.00 90.00 90.00 336993 (24158)

Unique reflections

46232 (2926)

71317 (6489)

32295 (2349)

26214 (1898)

Multiplicity

3.0 (1.9)

4.0 (3.6)

12.9 (12.3)

12.9 (12.7)

Completeness (%)

0.90 (0.57)

0.95 (0.87)

99.9 (99.9)

99.9 (99.8)

Mean I/sigma(I)

7.15 (1.31)

8.43 (1.31)

15.7 (3.3)

15.8 (3.8)

Wilson B-factor

43.9

29.31

55.8

45.9

Rmerge

0.08522 (0.4577)

0.1158 (0.9735)

0.143 (0.822)

0.132 (0.746)

Rmeas

0.1022 (0.5954)

0.1333 (1.138)

0.149 (0.858)

0.137 (0.778)

CC1/2

0.994 (0.732)

0.995 (0.523)

0.996 (0.901)

0.996 (0.881)

No. Sites

-

-

33

-

Figure of merit acentric (centric) Phasing Power

-

-

0.35491 (0.11335)

-

-

1.473

CC*

0.999 (0.919)

0.999 (0.829)

Reflections used in refinement

46206 (2922)

71312 (6489)

Rwork

0.2018 (0.2967)

0.1903 (0.2844)

Rfree

0.2586 (0.3716)

0.2356 (0.3249)

CC(work)

0.950 (0.820)

0.946 (0.641)

CC(free)

0.914 (0.591)

0.927 (0.577)

Number of nonhydrogen atoms macromolecules

8784

8634

8518

8112

Protein residues

1050

1008

RMS (bonds)

0.01

0.014

RMS (angles)

1.54

1.41

Ramachandran favoured (%) Ramachandran allowed (%) Ramachandran outliers (%) Rotamer outliers (%)

96

97

2.8

2.5

1.1

0

2.3

2.8

Clashscore

9.77

5.43

Average B-factor

52.99

36.33

macromolecules

53.07

36.05

solvent

50.5

40.74

Unit cell (Å, °)

Dcc1

Peak

Dcc1

90-380

Ctf18C-Dcc1-Ctf8

Space group

Native

90-380

Dataset

Inflection

0.391

2