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H. H. E. E. RBF1 ORF. UFR. DFR. HPT. #1. #2. #3 primer set #1. #4. #2. S8 Fig. Construction of RBF1-disrupted lines without GFP (∆rbf1-2). ∆rbf1-2. UFR. DFR.
A

pCAMBIA-RBF1-KO2 E

E

E

E

UFR

Bar

WT

E

HPT

DFR

H

E

H

UFR

primer set #1

E

DFR

RBF1 ORF

#4

∆rbf1-2

#2

E E

H

UFR

E

HPT

DFR

primer set #1

#2 #3

B

Ectopic insertion H

E

H

RBF1 ORF

UFR

primer set #1

#4

E

E

UFR

Bar

DFR

E E

E

#2

E

HPT

#1

DFR

#2 #3

C

primer set #1 kb 2.7

M 1 2 3 4

#2

#3

#4

1 2 3 4

1 2 3 4

1 2 3 4 M Lane 1: WT 2: ∆rbf1-2 line 1 3: ∆rbf1-2 line 2 4: ectopic M: size marker

1.9 1.5 0.9

D

∆rbf1-2 WT

∆rbf1-1

line 1

line 2

6 dpi

S8 Fig. Construction of RBF1-disrupted lines without GFP (∆rbf1-2).