Additional File 5.pptx

Addi$onal
 File
 5:
 Protein
 sequence
 alignment
 of
 Flagellin
 (FliC/FlaA)
 of
 P.
 aeruginosa
 strains
 used
 in
 this
 study
 (AES_1954,
 PA1092,
 and
 PA14_50290)
 and
 including
 an
 addiEonal
 sequence
 from
 strain
 PAK
 with
a
known
type
A
flagellin.
 The
 flagellin
 sequence
 of
 strain
 AES‐1R
 has
 higher
 sequence
 similarity
 with
 the
 shorter
 A
 type
 flagellin
 of
 strain
 PAK
 (95%),
 while
 the
 type
 B
 flagellins
 of
 strains
 PA14
 and
 PAO1
 are
 almost
 idenEcal
 with
 only
 a
 single
 amino
acid
difference.



AES_1954 PAK FlaA PA1092 PA14_50290

MALTVNTNIASLNTQRNLNNSSASLNTSLQRLSTGSRINSAKDDAAGLQIANRLTSQVNG MALTVNTNIASLNTQRNLNNSSASLNTSLQRLSTGSRINSAKDDAAGLQIANRLTSQVNG MALTVNTNIASLNTQRNLNASSNDLNTSLQRLTTGYRINSAKDDAAGLQISNRLSNQISG MALTVNTNIASLNTQRNLNASSNDLNTSLQRLTTGYRINSAKDDAAGLQISNRLSNQISG ******************* ** .********:** **************:***:.*:.*

60 60 60 60

AES_1954 PAK PA1092 PA14_50290

LNVATKNANDGISLAQTAEGALQQSTNILQRMRDLSLQSANGSNSDSERTALNGEVKQLQ LNVATKNANDGISLAQTAEGALQQSTNILQRMRDLSLQSANGSNSDSERTALNGEAKQLQ LNVATRNANDGISLAQTAEGALQQSTNILQRIRDLALQSANGSNSDADRAALQKEVAAQQ LNVATRNANDGISLAQTAEGALQQSTNILQRIRDLALQSANGSNSDADRAALQKEVAAQQ *****:*************************:***:**********::*:**: *. *

120 120 120 120

AES_1954 PAK PA1092 PA14_50290

KELDRISNTTTFGGRKLLDGSFGVASFQVGSAANEIISVGIDEMSAESLNGTYFKADGGG KELDRISNTTTFGGRKLLDGSFGVASFQVGSAANEIISVGIDEMSAESLNGTYFKADGGG AELTRISDTTTFGGRKLLDGSFGTTSFQVGSNAYETIDISLQNASASAIGSYQVGSNGAG AELTRISDTTTFGGRKLLDGSFGTTSFQVGSNAYETIDISLQNASASAIGSYQVGSNGAG ** ***:***************.:****** * * *.:.::: **.::.. . ::*.*

180 180 180 180

AES_1954 PAK PA1092 PA14_50290

AVTAAT--------ASGTVDIAIG-----------------------------------AVTAAT--------ASGTVDIAIG-----------------------------------TVASVAGTATASGIASGTVNLVGGGQVKNIAIAAGDSAKAIAEKMDGAIPNLSARARTVF TVASVAGTATASGIASGTVNLVGGGQVKNIAIAAGDSAKAIAEKMDGAIPNLSARARTVF :*::.: *****::. *

196 196 240 240

AES_1954 PAK PA1092 PA14_50290

------ITGG---------------SAVNVKVDMKGNETAEQAAAKIAAAVND------------ITGG---------------SAVNVKVDMKGNETAEQAAAKIAAAVND------TADVSGVTGGSLNFDVTVGSNTVSLAGVTSTQDLADQLNSNSSKLGITASINDKGVLTIT TADVSGVTGGSLNFDVTVGSNTVSLAGVTSTQDLADQLNSNSSKLGITASINDKGVLTIT :*** :.*. . *: .: .::.: *:*::**

228 228 300 300

AES_1954 PAK PA1092 PA14_50290

----ANVGIGAFT---------------DGAQISYVSKASADGT---TSAVSGVAITDT----ANVGIGAFS---------------DGDTISYVSKAGKDGSGAITSAVSGVVIADTSATGENVKFGAQTGTATAGQVAVKVQGSDGKFEAAAKNVVAAGTAATTTIVTGYVQLNSP SATGENVKFGAQTGTATAGQVAVKVQGSDGKFEAAAKNVVAAGTAATTTVVTGYVQLNSP ** :** : ** : ..:. *: *: *:* . ::

265 268 360 360

AES_1954 PAK PA1092 PA14_50290

---GSTGAGTAAG----TTTFTEANDTVAKIDISTAKGAQSAVLVIDEAIKQIDAQRADL ---GSTGVGTAAGVAPSATAFAKTNDTVAKIDISTAKALSRRAGDRTTAIKQIDASVPTS TAYSVSGTGTQAS-QVFGNASAAQKSSVASVDISTADGAQNAIAVVDNALAAIDAQRADL TAYSVSGTGTQAS-QVFGNASAAQKSSVASVDISTADGAQNAIAVVDNALAAIDAQRADL . :*.** *. .: : :.:**.:*****.. . *: ***. .

318 325 419 419

AES_1954 PAK PA1092 PA14_50290

GAVQNRFDNTINNLKNIGENVSAARGRIEDTDFAAETANLTKNQVLQQAGTAILAQANQL VAVQNRFDNTINNLKNIGENVSAARGRIEDTDFAAETANLTKNQVLQQAGTAILAQANQL GAVQNRFKNTIDNLTNISENATNARSRIKDTDFAAETAALSKNQVLQQAGTAILAQANQL GAVQNRFKNTIDNLTNISENATNARSRIKDTDFAAETAALSKNQVLQQAGTAILAQANQL ******.***:**.**.**.: **.**:********* *:*******************

378 385 479 479

AES_1954 PAK PA1092 PA14_50290

PQSVLSLLR PQSVLSLLR PQAVLSLLR PQAVLSLLR **:******

387 394 488 488