FK H. HSPAN2. 279 KR Y H R A M K Y S K G - L E - - - - - - D N F E S R N - A. TriadPAN2. 470 I N K Y E P P A Y S R P L I S. FT P F. ScPAN2. 394 H G G A SGL ...
Supplementary Figure S1. PAN2 sequence alignments. PAN2 sequences were aligned using ClustalW in DNAStar with default settings. Residues that are identical to the majority sequence are white on a black background. Accession numbers or locus designations from genome databases are indicated. The first page shows an alignment of the catalytic domain. The filled stars indicate catalytic residues and the open stars active site residues (see legend to Figure 1). Sc: S. cerevisiae, YGL094C; Hs: Homo sapiens, BAD02263.1; Triad: Trichoplax adhaerens, jgi|Triad1|30968|e_gw1.15.125.1; Chlamy: Chlamydomonas reinhardtii, Chlre3|170081|fgenesh2_pg.C_scaffold_12000140; Dd: Dictyostelium discoideum, DDB0190477; Mob: Monosiga brevicollis, jgi|Monbr1|26638|fgenesh2_pg.scaffold_15000072; Tb: T. brucei, Tb927.6.1670; Micp: Micromonas pusilla, jgi|MicpuC2|6335|gw1.2.610.1; Aure: Aureococcus anophagefferens, jgi|Auran1|14382; Naeg: Naegleria fowleri, jgi|Naegr1|33522|e_gw1.23.65.1; Thp: Thalassiosira pseudonana, jgi|Thaps3|268896|estExt_thaps1_ua_kg.C_chr_50163ds Gene locus numbers of PAN2 from other trypanosomatids are: T. cruzi Tc00.1047053507017.120; T. vivax - (tviv849h01.p1k_4); T. congolense congo1300a02.p1k_13, and Leishmania major - LmjF30.0250.
Exo I 972 721 908 756 1272 1133 836 58 1 1 36
G G A A A A A A
L L I I I I L I
D D D D D D D D
A T A A T A A A
E E E E E E E E
F F F F F F Y F
V V V V V I V V
T S S A S S V C
L L L S I L L V
N N Q S G R D Q
E Q S P P A W A
E D E P E A N E
E E L E E D K E
A A C S T T R S
E E E R E E Q H
L I I A V L C I
R H D S R V T
S S H S S T F
D D Q D S D S
G G G G G S G
T T I K H P S
K R R R R G K
S S S V N N E
T T I I T L I
I I I L I I A I
K K R K Q R Q S
P P P Q P Q Q E
S S K S G A R A
Q Q R R N F H R
M M T L F V M M N
S S A T S T A S A
V V L L L P L L L
A A A A A A A A A
R R R R R R R R R
I I I V V I L L L
T S S A S S S S S
C V I V I V C V I
V I I V I V V V I
R Y R A R A L D D
G G G A E A S C
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
1012 761 948 788 1312 1173 876 58 11 1 76
Q S E E N S S N R
E P A T
G G G G G G P E N
P P E P E Q E -
N K L L L G G -
E R Y A K D D -
G G G G G E E -
I T V S T R R R
P A P C A T T V I V
F F F C F I I I L L L
I I V V F V V I F I I
D D D D D D D D D D D
D D D D D D D D D D D
Y Y Y Y Y Y Y Y Y Y Y
I I V I I I V I V I V
S S V R Q K H R L S L
T T N A S N T A Q T P
Q S T V I T P V T A S
E E N E E E E E E E E
Q Q H P P Y A P T K P
R -
V V I T V V I V V I V
V V E Y T A L H V G T
D D D D D D D D D D D
Y Y Y Y Y Y Y Y Y Y C
L L L L L V V L N M L
T T T T T T T T T T T
Q E R R R Q Q R R R R
Y F Y W F F Y F F F F
S S S S S S S S S S S
G G G G G G G G G G G
I I I I I I I L V L I
K R L C N S H I A K R
P P P P P P P P A A E
G G G G G G G G E G S
D D D D D D D D D D D
L L L L L L L L L L L
D D D D D T D D D D S
A P P P P P P P P I A
N -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
1049 798 986 826 1345 1210 913 87 45 36 110
N -
K M E Q K A L N A K K
I V K V L T E T T S K
S S S S S S S S S N S
S T T R T T S G K T P
K K K H K K K H H H H
H Y R Y N H C H D H H
L L L T V L L I L V L
T T V T I T T T Y T V
T T R T T T S K R D T
L L R L L L L L R I T
K K N K K K K K A K Q
S A V H S S A H V N E
T S V T C S T A V T A
Y Y Y Y Y Y Y Y C Y Y
L R R L L V L L M L L
K K K K K K K K K K K
L L V L L A L L L L V
R L W K R S R R R R R
F C L W Y W A Y R Y L
L L L V L I L L L L L
I I M R V T V V V L M
D D Q G D P D D D D E
I R L P Q L R V A V R
G G G G G M S G G K G
G L K -
K -
A -
M -
V -
G E -
A R -
E G -
V V C L V V V C C V C
K I V K K I T V V K L
F F F W F F F F L F F
V V V V V V V V V I V
G G G R G G G G G G G
H H H G H H H H H H H
G G G P G G G G S G G
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
1080 829 1017 861 1376 1249 944 118 76 69 141
L L L G L L L L L L L
Q R N A K K H K Q P S
K K N G K K Q Q S N Q
D D D R D D D D D D D
F F F L F F F F F F F
R R K A R K R K R K R
V V H A I V V M V I V
I I I V I L C I M I L
N N N G N N N N N N N
L I I V I M I I V I I
M K N Q Y T A T A F S
V V V G V V V V V V I
P P P R P P P P P P P
K N R R P S P P P K A
Q -
G -
D -
E -
G -
D E N G N K N A D E D
Q Q Q Y Q Q Q Q Q Q Q
V V I L I I M I V I V
L I R L I L I V I I I
D D D D D D D D D D D
T T T C T T T T T T T
V V A G V V L V A V A
Y E I C E N T E Q E E
L I Y V L L L L L L I
F Y F F F F F F F Y F
H H L V Q Y H H S R H
M L Q G L L R F R L Q
P E G H K P P K P P T
R R D N K G R G N N
K Q K L Q Q G Q E Q Q
R R R R R R R R R R R
M Y Y K K F Y K K K Y
I L L L L L L I I I
S S S D S S S S S S S
L L L F L L L L L L L
R R R R R K R R R R R
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
1115 864 1051 900 1411 1284 979 153 111 104 176
F F Y C F F F F F F Y
L L L I L L L L L L L
A A A N A A A A C C T
W W Y M Y W Y S S K N
Y Y V V I Y H V Y Y Y
F F L V L F V L L L V
L L L P L L L L L L L
D N G P K G G D D N G
L R M E I V E L H Q R
K N N Q D V R D V D D
I I I L I M V I I I M
Q Q Q G Q Q Q Q Q Q Q
G T E S D E A D Q Q
E E G E D G T D D E
T T N T A E T N T V
R -
A -
G -
H H H H H H H H H H H
D D D D C D D D D D D
S S S A S S S S S S S
I I I I V V V I V I I
E E E E E E E E E E E
D D D D D D D D D D D
A A A A A A A A A A A
R V H R K R R R R R R
T T T T T T T V A T A
A A A A A A S A A A A
L L L M M L L I L L Y
Q D I R D A R R R R E
L L L L L L L L L L L
Y Y Y Y Y L Y Y Y Y Y
R D K H K D R E R K I
K K K K A V K K L K K
Y Y Y Y Y Y Y Y Y Y A
L T L L L E Q L Q L L
E N H E Q E Q D K E R
L T L L L W L L L L L
S I K T V S K N K K K
K K E A R R E E A Q N
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
1152 901 1088 934 1448 1324 1016 190 148 141 213
N M K N N D E I E K E
G G A K S G P G N G
T N I D E S K G T T K
E -
P -
E -
S T T A -
F F F F F W F F L F L
H R E E D N D Q R T D
K Q K R E D T A E S E
V K V V T I V K T T F
L L L L L L L L L L L
K I N N N Q D L G Q V
G A S E K K K E R D A
L C V M I L L I V I L
Y Y Y Y Y Y L Y Y Y Y
E E E A Q D A R D T S
K T E W I L T H H L H
G G G G G G G G G G G
R R R K R R V K R R H
K K A A S I E Q K Q A
M C H N L H T
D N N G S G S
W W F W F F W
K K K D K K Y
V A V P V P V
P P P M P P P
E T E A E A E
P T T P N G
E T S L N A R
G Q T S P K N A A V HsPAN2 TriadPAN2 K G ScPAN2 Q Q Q G L G Q Q G L Chlamy PAN2 I E L Q S L P Q I P DdPAN2 R E H C P F H E Q Y MobPAN2 D S F S S P L L E P TbPAN2 MicpPAN2 T E W T I AurePAN2 T N W V NaegPAN2 N Q F K L G V A D K ThpPAN2
Alignment Report of PAN2.meg ClustalW (Slow/Accurate, Gonnet) Tuesday, October 28, 2008 10:58
Page 1
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Majority 10 1 1 1 1 1 1 1 1 1 1 1
M -
S -
I -
R -
A -
S -
V -
G -
A -
H -
20 M P -
C F -
G P -
S L -
S R -
N K -
T F -
G R -
V I -
Y V -
30 G T -
G D -
Q F -
E V -
K T -
N H -
Q I -
N W -
H L -
Q L -
40 Q C -
P L -
Q S -
H L -
G S -
L L -
L S -
Y L -
S S -
N L -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Majority 50 1 1 1 1 31 41 1 1 1 1 1
Y C -
Q L -
Q S -
D V -
H S -
L L -
I S -
R L -
G S -
S L -
60 P K -
L P -
Q F -
S S -
Q F -
T S -
L N -
S R -
Y P -
N L -
70 L S -
G V -
N S -
Q L -
S S -
H L -
N S -
N L -
N S -
N L -
80 N S -
N L -
N S -
N V -
N S -
N L -
N S -
N L -
N S -
M N L -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Majority 90 2 1 1 1 71 81 1 1 1 1 1
N N S -
F N V -
E N S -
G N V -
L N S -
D N V -
P N S -
G N V -
L N S -
A N L -
100 E N S -
Y N L -
A N S -
P Q L -
A Q S -
M P L -
H P S -
S Q L -
A T S -
L Q L -
110 D Q S -
P Q L -
V Q S -
L S L -
D S S -
A P L -
H S S -
L P L -
N S S -
P P L -
120 S S S -
L P L -
L N S -
Q N L -
N N S -
V Q L -
E P S -
L F L -
D M Y S -
P N N L -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Majority 130 42 1 3 1 111 121 1 1 1 1 1
E N P S -
G W N L -
V Q I S -
A H N L -
L F G S -
E F G L -
A N A S -
L N P L -
P P P S -
V V P L M -
140 Q D Q S S -
E L P L V -
S S I S P -
V E Y L W -
H H N S K -
I L P L V -
M K M N A R -
E K Y N L G -
G P S G S S -
V Y S L I V -
150 Y F Y Y F Q -
R G G V A -
F M Y F F -
D S T P V -
N P G -
R H -
Q -
T -
G -
E Y G -
160 L E A -
A I T -
V D A -
A Q S -
P P A -
S L E M -
E D D D -
L D A D E -
H K S G A -
S Y Y L -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
Alignment Report of PAN2.meg ClustalW (Slow/Accurate, Gonnet) Tuesday, October 28, 2008 10:58
Page 2
- - - - - - - - - - - - - - - - - - - - - - - - - - - F X - - - - - - W - X - - Majority 170 68 1 31 20 151 156 17 1 1 1 1
P Y M -
D P -
G F -
T T -
W S -
F F -
E -
M -
R -
V -
180 V -
P -
E -
Y -
V P G -
V S H -
A S S -
E N P -
V S E -
G Q Q -
190 V S N A -
P E A P P I -
V I V I I V -
S T T T A N -
V A A S R A -
S I M L V I -
H S Q K V E -
F F F F F F -
D D S D D D -
L E P H Q P -
200 H K H W V D -
E A Y E F V -
E N E E N E -
M L A L L L -
L I M L L L -
W W W W W W -
V S A V V V -
G G G G L G -
S D T Y D D -
H S E Q E S -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- G - - - X - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Majority 210 94 1 50 41 191 188 36 1 1 1 1
G Y A N R F -
G G G G G G -
H C H K Y R -
A I L I I L -
T S Y S S M -
S S C S S S -
F Y L L Y F -
F D Q I N T -
G P A N T -
P T P P E Q -
220 A F S T S S -
L Q L L F Q -
E L Q D Q T -
R Y P R R D -
Y T Y F Y T -
S R A S S L -
S Y H S A S -
F R W F W W -
Q G Q F T A -
V H A V L V -
230 N I H D Q Y -
G G P K P S -
S G H T A S -
D N G T F -
S G A -
V H A -
K D S -
D G T -
I S R -
L A P -
240 S S D I -
D H P E K F -
I R V I P G -
R D L K T I -
Q G E D F F -
I I V L M F -
Q L A L T L -
S S A V S R -
L I A D T M -
E S G S V G -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Majority 250 127 1 90 75 223 227 76 1 1 1 1
N E D E H N -
G D G G G E -
I S V I L T -
L L M I I V -
F H S S V G -
L F L L I M -
T A S S H V -
K N A K A A -
N R H T N D -
N R R A Q H -
260 L G L L I D -
K V C S M V -
Y T L Y L I -
M K H H F R -
A L G S S G -
R N L R V Y -
G L G G G K -
G T G G G R -
L S A A V G -
I I P P Q G -
270 I D R I H A -
F I W Y M Q -
D A S Q R C -
Y A L I I M -
L F N K V Y -
L S D N L C -
D E D D R P -
E L S R G L -
N N G M D P -
E T A K K P -
280 D M F D T Q -
M C S F G S -
H Y A N I Q -
S S V A T D -
L P C I C H -
L H L S A I -
L S D F D E -
T L A S A L -
K G N S F -
N G F P Q -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - X - X - - Majority 290 165 1 130 115 263 267 116 1 1 1 1
D G E H A -
S N G N N N -
S T Q G -
D -
T -
G -
V -
T I E R V -
L Y I Q S -
L C V I I -
300 V G I I V -
G G G C G -
G D G S G D -
L N G D G V -
Q T G Q A G -
N N V N G L -
H W A T A T -
I G P L L R -
L I V Y H M -
E A G V V S -
310 I S L V L I -
D I A D D S -
L D G F L D -
N L A F T G -
T N R T K T -
V R A G Q A -
Q G V K Q C -
E C L V F N -
T V L G V V H -
Q L D R K K V -
320 K R S S E S T -
Y Y L S V S V -
A S L A T P P -
V N N R M L L -
E K Y M S P M -
T S S S S D E -
P S L T G -
G G K V G G K -
V V D V D V -
T K V K I V -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
Alignment Report of PAN2.meg ClustalW (Slow/Accurate, Gonnet) Tuesday, October 28, 2008 10:58
Page 3
- - - - - - - - - - - X - T - G - X - - - D - - - - - - - - - - - A X X G - - - Majority 330 201 10 164 145 298 303 156 1 1 1 1
I L G T Q S -
M M T I T L -
R C G N L R -
Q S R R R Q -
T N V C T -
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R K R R K -
F Y V S Q W -
F V L L L V -
F C S W I I -
340 C C I C V T -
G G G G A G -
H T R T Q T A -
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G G G Q G T G -
K K T E E E G -
V V V V I L I -
S T D L S L V -
L L L G M M V -
350 R H L L L I R -
D D D L D D D -
L P P G P G A -
R S T G R R N -
T T S E T Q E -
F L N G W H L -
K Q R V R V Q -
V C T T I V V -
E I I L E R V -
H N K L S L A -
360 E A S R S R S -
F I F G L T I -
D D N P P K P -
A A A A A A S -
F H H S H H S -
S S S R K S R -
G G A G G G N -
S S S A D G R -
L I I L I T V -
S S S V K A L -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - D - - X X - X X - X X - - X - X - - - - - - - - - - - X Y D - X - M X - X - Majority 370 241 43 204 178 337 343 196 1 1 1 1
D D A A S N A -
F I M A V L M -
D D D T D D D -
V V L S I L A -
H S R L K R D -
G D D G G D D -
N N N R D N E -
L F T L L T T -
L L L A L A V -
A V V L V V I -
380 A T T I S T A -
C C V D C C A -
G G G G G V -
K -
F F K C T V -
S S S P S A E -
S T K R P T R -
R R R A R G N -
L H F K L R M -
T S Y L G L T -
390 G H N R Q D -
L L L A M N -
A N Y D Y N -
C C A S I I -
D D D A D H -
R R P L P V S -
F F F I I I S -
L L V A V D V -
K M N A R V R -
V M V P V S V -
400 Y Y Y G Y A Y -
D D D G D G D -
L L L V I V V -
R R R A K Q R -
M S T A T A K -
M M M L M L M -
R R R D R C A -
A A Q A S L E -
I L L R L L P -
T N P G P S L -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
X - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Majority 410 280 82 243 215 376 383 230 1 1 1 1
P P P D P N N -
L I V T I H T -
Q Q S V Q M I -
V V F A Y V N -
H H S A K S G -
V F K A R A I -
D D G G P P -
R -
A -
F -
420 V -
I -
T -
H -
T -
Q -
Q -
T A S -
P P T P L D -
A T M Y S M S -
430 F L G G I R I -
L L S M V N S -
R K G R K L Q -
F F G A F A V -
I M A G H N R -
P P D T P R R -
T N F V I S Y -
Y Y V V F A R -
T T Q A Y P D -
S S L E S P P -
440 R R H N T T F -
L L P C V L G -
A A L V V I V -
I V L K V P L -
I V P L V Y P -
S S T F S P D -
Q H V D E T R -
S T M V S V A -
G G I R S Q F -
V A N D V -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - X X - - - - - Majority 450 308 110 273 243 404 423 260 1 1 1 1
Q Q A G Q G L -
C F S L I G T -
Q Q S R Y R S -
F I S L L V R -
C C G L C T G -
E E S Y D F F -
P P F T V V H -
G D L Q C I -
F P G G V -
T V I T D S Q -
460 G P D S I P M -
L L L G Q S D -
A T S P S D Q -
N P N P Q A T -
P S P A S A T -
A S T A P V P -
D L L L P A V -
I I R A Y F Y -
F V T F L G S -
H Q Q H Q D S -
470 V Q Y P I S S -
N I V P D H G -
P E H P S G L -
V S P L L M F -
G T C G F V D -
P G Q S G S D -
L Y S A Y I A -
L P I C L W C -
M L K L Q T T -
T A K L S L S -
480 F F L V F N V -
D D C A D G S -
V V L L I M V -
S S S P A P S -
A P P S P L P -
S S N A T A N -
K A G A G F A -
N -
Y -
G -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
Alignment Report of PAN2.meg ClustalW (Slow/Accurate, Gonnet) Tuesday, October 28, 2008 10:58
Page 4
- - - X - X X - - X - - - - X - - - - X - - - - - - - - - - - - - - - - - - - - Majority 490 343 146 310 280 441 463 297 1 1 1 1
Q Q D E M M -
A V V C P C -
L F L F M L A -
A A G C A P A -
F F I L F V I -
G G L A G V G -
D D E D D N N -
S S A T S D D -
E E D A G I K -
G G N S G R G -
500 C Y H G L L H -
V L L G V H F -
H H D T H P M -
L V T S Q H A -
W W W Q W R L -
T T R V A V G -
D N R Y E H H -
S R S Q K F P -
P E S A E D A -
E D N D D D T -
510 P A N M I L R -
S M M A H E E -
F M G S V A D -
N N M D N P Y -
P N F Q I A V -
Y F T L N A M -
S G N S P Y S -
R Q T T Y M R -
E P P A P L -
T T E C T V -
520 E E M L E N F -
F F L S C S V -
A A A P V T Q -
L A Y S P K P -
P D P G F T K -
C D D T S R -
L Y L Q D P -
V E F E L W V -
D N N D S P N -
S R D D R T P -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - X - - - - - - - - - - X - P - - - - - - - X - - - - - - W - - - Majority 530 383 184 350 318 481 503 335 1 1 1 1
L L I S P D V -
P P T F Q K F -
P Y S A K T T -
L L D A M M A -
D D G A D Q R -
W I P V E F W -
S N I Q Y Q E -
Q D S Y S R D -
D P V P P P K -
L F D P L P E -
540 L V D P S I G -
P R E S D R D -
L L T P V V T -
S S Y P I P F -
P P F E A -
L G D A F -
S H Q M F -
L T S T P L D -
I V V G P G E -
P P G P P R S -
550 V M M P V A V -
P D P P P T P -
L Y Y G P W -
T T Y L Y M -
T Y L A D N V -
D D D S Q S E -
T K K F E E -
L L L P R L -
L L L L G A -
S S S S H S -
560 A A N -
W P W -
P N P -
P V P -
V L E -
D D V D Y N N -
W W F W W W Y -
P S K E K K M -
A N S P P P I -
A E E A N F L -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - X - - - - - - X - - - - - - X - - - - - - - - - X Y - P - X - - - - - - - Majority 570 415 216 390 349 516 543 373 1 1 1 1
N L G E L Q Q -
S T T S S V V -
A E I S Y Q A -
P V P A P Y P -
A K Q V V S S -
P D L G G S T -
R R T L L A C -
R R G P P A C -
A L K P T L V -
P A S R R S N -
580 P P P V P V G -
V I L L I S E -
D D P D N S S -
A P S P S P W -
S S T -
E K G A S K Y -
I I K M L L E -
L L L R L K P -
R E K R V G N -
T S S E H K R -
590 M M N L L Q W -
K K L R K S G -
K M A V Q R L -
V N V V H F R -
G N I D D H R -
F F S G F G S -
I I S V V I D -
G G Q G G D S -
Y Y N Y Y Q Y -
A A E L I T L -
600 P P K P S P P -
N A L N N L D -
P P S P T G P -
T G R -
Q I D -
E S K -
R V F R L -
T N P K A -
R Y L Q P -
L K L I L -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - X - - - - - - - - - - - - - - - - - - - - Majority 610 451 252 430 381 555 576 413 1 1 1 1
R R R R L -
N N Y G P -
Q D K N -
Q A R A P -
I Y T Y Y -
P L K E P -
Y F Y D Y -
R R G G N -
L N M S L -
K V R K M -
620 E T N K L -
S S A H T R G -
D L I Y L M N -
S H P S S D -
E K D R N D D -
F L Y A L L P -
D D V K T E A -
S A C S I G A -
F L L L S I V -
S S R A S S Q -
630 Q E D D M A H -
V Y I M S M L -
T Y R A H I L -
E G K R I P L -
S Q R E -
P I P L -
V T N -
G S K -
R G E -
E L K -
640 E E K -
E T R -
P S R -
H D Q -
L I S -
H Q T -
M E T F -
V I Y R -
S P T P G -
S G -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
Alignment Report of PAN2.meg ClustalW (Slow/Accurate, Gonnet) Tuesday, October 28, 2008 10:58
Page 5
- - X - - V - - X - - X L - - - - - - - - - - - F D X - - Y N - T - - F X G L E Majority 650 489 279 470 394 582 589 452 1 1 1 1
K K I H K R V -
K R N G S H A -
Y Y K G Y Y D -
R H Y A R R N -
K R E S W R Y -
V V V G G V V -
T A P L E R P -
I M P N M N Q -
K K A G K K D -
Y Y Y G F D A -
660 S S S P K D A -
K K R R K K N -
L L L L F L L -
G G P N G D V -
L K C -
L L T W V Y -
E E S E E G -
G -
R -
F -
670 G S -
T H -
D Q -
D D N E D R -
F F F F F F F -
D N D D D D E -
F F F F M Y W -
K E T A V Q E -
H S P A E H K -
Y Y F H Y Y Y -
680 N N N N N N N -
K R N S K E -
T T T T T T -
L N E G K N R -
R -
F F Y F F F I -
A A S A A V P -
G G G G G G K -
L L L L L L V -
E E D E E E V -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - P N - Y X N - - - Q - L X F X P X - R - - - - - - X H - C X - E F C L - C Majority 690 521 311 509 428 614 618 486 1 1 1 1
P T P N N P V -
H D D D I T G -
I I V L L A L -
P P D P P P D -
N N N N N N N -
A A H A S T S -
Y Y Y Y Y Y F -
C C T C T C P -
N N N N N N -
C S A A S A -
700 M M I L C M -
I L I F I L -
Q Q Q Q Q Q -
V V L A N T -
L L Y L L L E -
Y Y R Y Y F C -
F F F F F Y W -
L L I L I L I -
E R P P P T T P -
P P E E Q P T L -
710 V M M F I V L I -
R C F R K R L R -
N G Q -
F L S -
C I V V E S L -
L G V L T L Y -
I L G A M L L -
Q Q C H L M C T -
N N L V N Q H L -
H H K P H H P L -
720 L S D E L Y K G -
C C E P C C E I -
Q D N D T E F D -
K K F A R D P A -
E E E E N E V E -
F P T F Y F R F -
C C T C C C R -
L F L L L L V -
A A L T S A V -
C C T C C C V -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
E L G F L F - M - - - - - - - - - - - - X X - - - - - - - - - - - - - - - - - - Majority 730 559 349 549 468 652 656 520 14 1 1 1
E E D E E E H -
L L L L L L H -
G G G G G M I -
F F Y F F F C -
L L L L V L A -
F F F D F F K -
H R D A Q D E -
M M M T M M Y -
L L M N M M C -
D D E A D A I -
740 L Y R R K K A -
S S S T C R C -
R K H Q N E -
G G G G T I -
D Q K N I A -
P P I I T F -
C C C V C V -
Q Q S D Q F -
G A S T A S -
N N S S T N -
750 N N N N N M -
F F F F F M -
L L Q I V M -
R R A R R T -
A A S V A S -
F F L L F V -
R R K K R A -
T A S Q T G -
I I L I I L -
P P T P Q N -
760 Q -
A -
V -
N -
S -
G -
L -
I -
I -
T -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - X - - - Majority 770 589 379 579 480 682 696 550 14 1 1 1
E -
E -
E -
A -
D -
S -
P -
D -
T -
D -
780 L -
H -
L -
L -
T -
Q -
R -
W -
Q -
F -
790 F -
I -
V -
T -
Q -
L -
A -
R -
I -
V -
800 P -
R -
E E Q D -
A A T P -
S A D P A E P -
A G K S A E I -
L L R P L L V -
G G Q G S S Q -
L L L P I H V -
I I E F F S G -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
Alignment Report of PAN2.meg ClustalW (Slow/Accurate, Gonnet) Tuesday, October 28, 2008 10:58
Page 6
- - - - - - - - - - - - - - - X I A P - - X - - - - - - - - - - - - - - - - - - Majority 810 597 387 585 486 690 736 556 14 1 1 1
L L N R L D N -
A P G S S N -
D D E G G G -
S T P -
D E Q D -
E E E I S G -
A A H A P P L -
S Y L P D S I -
G K E Q P A R -
K N E P Q P T -
820 G A Y A I S M -
N N L P P T Y -
L L E A L V R -
A A S N T S C -
R H L A Q V P V -
L L C A L T S A -
I I I L I V A L -
Q Q R A A Q A A -
R S E P N P P P -
W W S A F S L A -
830 N H I L N N F -
R L E E R E E -
F F D A F A P -
I V F L L P I -
L L N M L P K -
T Q S G D R S -
Q Q S I Q E R -
L L E S I S D -
H S S F Q G E -
Q R I R K K A -
840 D D K E D V -
M T R L S K -
Q N T G -
M P R -
P P S -
I R -
P R -
T G -
Y G -
N R -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - X - - - X - X X - - - - - - - - - - - - - Majority 850 630 419 620 514 729 774 583 20 1 1 1
N A -
N G -
N R -
D S -
D R -
N K -
N D -
N A -
N P -
N T -
860 N D -
E Q N S -
L K L S -
E F N S -
I N T T N -
P R R N A R -
F M N M M V -
Q L T D R H T -
A L C T M M S -
Y Y S L N L A L -
870 R K Q T N E Q D -
G K L G N A L A -
A D I G N A V C -
G D K G N A L G -
G K E G N A L N -
S S E D T A E E -
S E A S N A A V -
F A Q V N A L V -
C T T R T A H -
S D V D N N R -
880 S N N G N D G -
R N A -
S C S -
I -
S -
N -
I -
G D H F N A L -
D G N Q D S Q -
S A I V T S D -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - X - - - - - - - - - - - - C - - - - - - - X - - X L - - L - X P - - - Majority 890 652 434 643 536 769 810 602 32 1 1 1
V W T D I P E -
I F L L I I K -
G L N Q D H E -
Q K Q K S Y -
L L C L L P -
F Y F F F F -
S E G G N -
C S L S T -
E K E I A -
M L T T P -
900 E E E T I -
N I S K -
C I S T -
S T N M -
L K I K T M -
C C R C C N -
R G T I S H -
E N V K -
C C C Y C C Q -
G E S P G Q P -
910 S N C A H S C -
E K D S Q S G -
T D H R Y T D -
V I Y E E V F -
R R D K K S D -
A T T A Q T K -
S S T A S T S -
S S V R R T I -
T T K A Q S A -
L L L A F L S -
920 L L L E Q T L V -
F Y P E Y Q F Q -
T T S A D V G A -
L L L R I L T A -
S T S Q V R E R -
Y Y I L Y F F L -
P P S G P P S G -
D S G L N E S L -
K I N N G -
S N G -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - A X C - - C Majority 930 689 473 680 560 807 849 634 40 1 1 1
S K K -
Q T V -
P V H -
N I R T G V -
K K L P T E -
D D Q P Q P P -
K K L G E E R -
T Q N A L E -
G A K A G D -
N K -
940 S S -
N -
E -
I -
K K N P G A -
N T G P D Q -
Y H Q M S L -
D S N R T R -
F F I S F F -
A C L S S -
950 Q S P N D F -
V V Y L L Y -
L L I L I W -
K L E K S D -
R S Y L S V -
S S A S V P -
I M M L L L A -
C S K L T M S -
L K N A K R A -
D T V A Q E I -
960 Q Q T A I Q K -
N N Q A K T V -
T V K T S D N -
Q F N R P K E A -
A A S A A A G R -
W W I W W Y L -
C C C V C C Q -
D E P D E S H -
T R T E K K L -
C C C A C C L -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
Alignment Report of PAN2.meg ClustalW (Slow/Accurate, Gonnet) Tuesday, October 28, 2008 10:58
Page 7
- - - - - - - Q - - - - X - L P - - - - - - - - - - - - - - - - - - - - - - - - Majority 970 719 513 717 585 839 881 661 42 1 1 1
E Q G R S K K -
K K K G T E Q -
Y Y T Y F F L -
Q Q E A S K E -
P P T L S V G -
T S I V T H H -
I T T K F V N -
Q Q Q K Q Q S -
T T E V K V N -
R R C R K L V -
980 N L T V Q E Q -
I V V P L A I -
R K K V K S K -
H N N C T T R -
L L L L L L L -
P P P P P P P -
D D N G -
I I I V -
L L L M -
V A C A -
990 I I I F -
N N N S -
C C A C -
E Q N P -
V V C L -
N D L T -
S N K R -
S E K D -
K E E R -
E G N H -
1000 A I E R -
D H E W -
F F Y D -
W W W A -
R K K R -
M Y Y M -
Q Q E R -
A D V L -
E M S P A H P -
V V V L N Q I -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - V X V X X X X - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Majority 1010 759 553 735 603 879 921 679 42 1 1 1
A A L L I S I -
F V S T I E V M
K I L V P E L V V
M L E N G H R S S
A I L V S V L V T
V L S A K A N V S
K N L V T T P R V
K D L G F S E G M
H R D D N K G D
G T S A R G T
G E S T A T -
1020 P -
S -
S -
I -
P -
L -
Q -
Q -
S -
Q -
1030 S -
S -
T -
T -
T -
T -
T -
S -
S -
T -
1040 S -
T -
I -
T -
V -
P -
P -
S -
T -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - X - - - Majority 1050 770 562 746 614 919 932 690 48 1 1 10
Y -
N -
N -
N -
N -
N -
N -
N -
N -
N -
N -
1060 N -
N -
N -
N -
N -
N -
N -
E E N A -
I I F A N A L -
S C S A N V K -
1070 K H N W S V P -
N I I W N M P -
K R R L T A R -
E F S P N P C -
F Q S A S A L -
A L K K Q E R -
L Y N G P L -
A I W I R -
D F L P N P -
W Y T A N C -
1080 K Y S P T D -
E D E V N V -
L L F L I L -
G N Y A S P -
S S G N T D -
P P S P P P -
E D N S -
G V G A -
V V K I -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Majority 1090 792 580 764 635 959 954 698 48 1 1 10
L T P -
V L D -
C S Y -
P P V -
P -
N -
F -
S A -
T -
P -
T -
1100 H -
Q -
T -
H -
Q -
S -
S -
T -
P -
I K -
1110 S -
T -
P -
T -
H -
Q -
I -
T -
T -
S -
1120 N -
S -
S -
T -
S -
S T -
N -
S -
S -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
Alignment Report of PAN2.meg ClustalW (Slow/Accurate, Gonnet) Tuesday, October 28, 2008 10:58
Page 8
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Majority 1130 796 580 764 635 999 961 698 48 1 1 10
T L -
S S -
L W -
S I -
P P -
S I -
S S E -
I L L -
E S R -
E I A -
L L L Q -
1140 K F I L -
N D N N -
N -
N -
Q -
L -
T -
T -
T -
T -
1150 T -
T -
T -
T -
T -
T -
N -
T -
A -
T -
1160 P -
I -
E -
E -
I -
V C T -
W W W R -
L Y L D -
P P N -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - X - D X A - - - - - - - - - - - - - - - - - - - - - - - - Majority 1170 807 586 764 635 1039 977 698 48 1 1 10
F L T -
S K K -
I F I Q -
R L R K -
M Q I A I L -
K E I C K V -
M V K V I I -
T L N Y D E -
K L K E D E -
N G A L N T -
K E V A G L I V
1180 G G L G Q V S S
L Y R S L P R I
D L S D F T D D S
V K T A I T A A T
C C A A T A K A G
N I S A E A D I -
W L E A Y T D E -
T L L Q P P S R -
D N K D N P N -
G G S N A -
1190 D T D P G -
E S T E E -
M H N K G -
Q I G S S -
W F R K Q -
H -
H -
V -
D -
P -
1200 G K -
P D -
A L -
R T -
A Q -
E E -
E A I S -
E R G N D -
H A K S -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - X - L - - - - - H - - X - - - - - - - - X - - - - - - - V - - - - - - - Majority 1210 842 605 786 660 1079 1006 708 54 1 1 16
G S D N -
V G G A -
Y P Q I -
V K R L V -
Y Y N Y Y Y -
D E S E E L -
L L L L L L -
M N S T F C -
A G S A A S -
T Y G C A N -
V V A V V I -
1220 V A G S Y I -
H H K G H H H -
I W I A I V L -
L L T G K R S -
D P D A D E N K
S N N R Q S D G
N V A -
K -
R -
I -
1230 R K N S P K D -
T I E Q K T D -
G Q T G Q C P -
G A R A G G G -
S Q L H N -
L I V C M -
V V V V -
A D S S -
H D Q I -
I E T I L N -
1240 K L Y G K H F -
V V V T V V V -
G M K G S P S -
E N K T D S H -
T A Y G F H Q -
Y I N R Y C R -
H D P G N T V -
Q S K H K G E -
R K E P E D D -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Majority 1250 878 629 817 690 1119 1042 736 54 1 1 18
K F -
K -
E -
E E -
S -
L -
E -
L -
L -
G -
S -
1260 N -
N -
N -
S -
S -
N -
S -
D -
N -
E -
1270 N -
N -
T -
N -
N -
T -
T -
T -
F -
N -
1280 N -
N -
N -
N -
N -
N -
N -
N -
N -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
Alignment Report of PAN2.meg ClustalW (Slow/Accurate, Gonnet) Tuesday, October 28, 2008 10:58
Page 9
- - - - - - - - - - - - - - - - - - - - - - W - L F N D F - X - - - - - - - - - Majority 1290 880 629 817 690 1159 1042 736 54 1 1 18
N -
N -
N -
N -
N -
N -
N -
N -
N -
N -
N -
1300 N -
K -
H -
R -
P -
G G N -
V V Y -
T R N Q I G D -
H H C G D S R -
Q T F P S R F -
1310 Q T K E T P S -
W W W W W W -
Y Y L V F V -
L L M L N L L -
F I F F F F V -
N N N N N N N -
D D D D D D D -
F F Y F F F Y -
L S L C K A L -
I I V V I V V -
1320 E T V N N T -
P P E E V K -
I I I C V S -
D P T S S D -
K P E P C D -
H V E T N E -
E D E D D Q -
A S A V A K -
V S L T V L -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - X - - X - - - - - - - - - - - - - - - P - - - - - - X - - - - - - - - Majority 1330 904 653 839 703 1199 1064 756 54 1 1 18
Q S K H L E -
F F M F Y V -
D S T D D S -
M L Y T T M -
N D P N T P -
W W W W W A -
K Q K K K L -
V I T T T R -
P P P P P S -
A C E S C H -
R -
1340 A -
I I I I L V -
L L I L Y V -
Y Y I Y Y S -
Y F Y Y Y F -
V I C N V Y -
K K D K Q A -
R A A A V K L -
N S E G V D D -
L V E G S S K -
1350 N F L I N L L -
S Q R P S E S -
R N K P S A T -
Y F P A T P P P
N E F E D K L L
F V T G Y Y
S T T A S S
V I A A
L G D A T P E D
N G T T N P D S
1360 I G Y Y I S G Y
K K S G P L E S
N I I N I Q R -
P W N Q P E P -
I I Y K P Q P -
E N D L H F N -
A F I P I I M -
F -
E -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - L - - - - L - - - - - - - - - - - - F - - - X - - - E X P X - G D L V Majority 1370 937 686 875 722 1235 1099 796 54 1 1 27
T -
L -
S S A S R G -
V I L T R P -
L I L I L L -
L L L Q T L L -
A A F Y K N I -
E D R K E D N -
A K D Q V P D -
S S Y G Y S V -
L L F A L L L -
1380 A N A M Q S A -
R K N A N V R -
K N G N N Q P -
Q P I L M R L -
R E P V P Q -
R T R -
D C N -
K I T K P -
T S A T S -
H T R P P L I -
1390 T S R F I V Q -
T S E R N P R -
F F Y F F A F -
I V K K T S K -
P T P P T S -
L L V P -
L P L D A L L -
M L T M T D R -
L E H V A R S -
N E D R N A H R -
1400 E D E M T S L L E
M L A A I I D V L
P P P P P P I P P
Q G K R K S K G
I K S P E A P K
G G G G N G E G
D D T T D D D D
L L L R L R L L
V V V F V V V V
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
A I D A E F V S L - - E E - E X - X X G - X - - I X P X X M S L A R I S V V R X Majority 1410 972 721 908 756 1272 1133 836 58 1 1 36
G G A A A A A A
L L I I I I L I
D D D D D D D D
A T A A T A A A
E E E E E E E E
F F F F F F Y F
V V V V V I V V
T S S A S S V C
L L L S I L L V
N N Q S G R D Q
E Q S P P A W A
1420 E D E P E A N E
E E L E E D K E
A A C S T T R S
E E E R E E Q H
L I I A V L C I
R H D S R V T
S S H S S T F
D D Q D S D S
G G G G G S G
T T I K H P S
1430 K R R R R G K
S S S V N N E
T T I I T L I
I I I L I I A I
K K R K Q R Q S
P P P Q P Q Q E
S S K S G A R A
Q Q R R N F H R
M M T L F V M M N
S S A T S T A S A
1440 V V L L L P L L L
A A A A A A A A A
R R R R R R R R R
I I I V V I L L L
T S S A S S S S S
C V I V I V C V I
V I I V I V V V I
R Y R A R A L D D
G G G A E A S C
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
Alignment Report of PAN2.meg ClustalW (Slow/Accurate, Gonnet) Tuesday, October 28, 2008 10:58
Page 10
S - G - - - G X X F I D D Y I S T T E P - V V D Y L T R F S G I X P G D L D P - Majority 1450 1012 761 948 788 1312 1173 876 58 11 1 76
Q S E E N S S N R
E P A T
G G G G G G P E N
P P E P E Q E -
N K L L L G G -
E R Y A K D D -
G G G G G E E -
I T V S T R R R
P A P C A T T V I V
F F F C F I I I L L L
I I V V F V V I F I I
1460 D D D D D D D D D D D
D D D D D D D D D D D
Y Y Y Y Y Y Y Y Y Y Y
I I V I I I V I V I V
S S V R Q K H R L S L
T T N A S N T A Q T P
Q S T V I T P V T A S
E E N E E E E E E E E
Q Q H P P Y A P T K P
R -
1470 V V I T V V I V V I V
V V E Y T A L H V G T
D D D D D D D D D D D
Y Y Y Y Y Y Y Y Y Y C
L L L L L V V L N M L
T T T T T T T T T T T
Q E R R R Q Q R R R R
Y F Y W F F Y F F F F
S S S S S S S S S S S
G G G G G G G G G G G
1480 I I I I I I I L V L I
K R L C N S H I A K R
P P P P P P P P A A E
G G G G G G G G E G S
D D D D D D D D D D D
L L L L L L L L L L L
D D D D D T D D D D S
A P P P P P P P P I A
N -
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- K T S T K H L T T L K S T Y L K L R Y L V D R G - - - - - - - - V K F V G H G Majority 1490 1049 798 986 826 1345 1210 913 87 45 36 110
N -
K M E Q K A L N A K K
I V K V L T E T T S K
S S S S S S S S S N S
S T T R T T S G K T P
K K K H K K K H H H H
H Y R Y N H C H D H H
L L L T V L L I L V L
T T V T I T T T Y T V
T T R T T T S K R D T
L L R L L L L L R I T
1500 K K N K K K K K A K Q
S A V H S S A H V N E
T S V T C S T A V T A
Y Y Y Y Y Y Y Y C Y Y
L R R L L V L L M L L
K K K K K K K K K K K
L L V L L A L L L L V
R L W K R S R R R R R
F C L W Y W A Y R Y L
L L L V L I L L L L L
1510 I I M R V T V V V L M
D D Q G D P D D D D E
I R L P Q L R V A V R
G G G G G M S G G K G
G L K -
K -
A -
M -
V -
G E -
1520 A R -
E G -
V V C L V V V C C V C
K I V K K I T V V K L
F F F W F F F F L F F
V V V V V V V V V I V
G G G R G G G G G G G
H H H G H H H H H H H
G G G P G G G G S G G
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
L K K D F R V I N I X V P P - - - - - X Q I I D T V E L F H L P X Q R K L S L R Majority 1530 1080 829 1017 861 1376 1249 944 118 76 69 141
L L L G L L L L L L L
Q R N A K K H K Q P S
K K N G K K Q Q S N Q
D D D R D D D D D D D
F F F L F F F F F F F
R R K A R K R K R K R
V V H A I V V M V I V
I I I V I L C I M I L
N N N G N N N N N N N
L I I V I M I I V I I
M K N Q Y T A T A F S
1540 V V V G V V V V V V I
P P P R P P P P P P P
K N R R P S P P P K A
Q -
G -
D -
E -
G -
D E N G N K N A D E D
Q Q Q Y Q Q Q Q Q Q Q
1550 V V I L I I M I V I V
L I R L I L I V I I I
D D D D D D D D D D D
T T T C T T T T T T T
V V A G V V L V A V A
Y E I C E N T E Q E E
L I Y V L L L L L L I
F Y F F F F F F F Y F
H H L V Q Y H H S R H
M L Q G L L R F R L Q
1560 P E G H K P P K P P T
R R D N K G R G N N
K Q K L Q Q G Q E Q Q
R R R R R R R R R R R
M Y Y K K F Y K K K Y
I L L L L L L I I I
S S S D S S S S S S S
L L L F L L L L L L L
R R R R R K R R R R R
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
F L A X Y L L G X D I Q X E T - - - H D S I E D A R T A L R L Y X K Y L E L K E Majority 1570 1115 864 1051 900 1411 1284 979 153 111 104 176
F F Y C F F F F F F Y
L L L I L L L L L L L
A A A N A A A A C C T
W W Y M Y W Y S S K N
Y Y V V I Y H V Y Y Y
F F L V L F V L L L V
L L L P L L L L L L L
D N G P K G G D D N G
L R M E I V E L H Q R
K N N Q D V R D V D D
I I I L I M V I I I M
1580 Q Q Q G Q Q Q Q Q Q Q
G T E S D E A D Q Q
E E G E D G T D D E
T T N T A E T N T V
R -
A -
G -
H H H H H H H H H H H
D D D D C D D D D D D
S S S A S S S S S S S
1590 I I I I V V V I V I I
E E E E E E E E E E E
D D D D D D D D D D D
A A A A A A A A A A A
R V H R K R R R R R R
T T T T T T T V A T A
A A A A A A S A A A A
L L L M M L L I L L Y
Q D I R D A R R R R E
L L L L L L L L L L L
1600 Y Y Y Y Y L Y Y Y Y Y
R D K H K D R E R K I
K K K K A V K K L K K
Y Y Y Y Y Y Y Y Y Y A
L T L L L E Q L Q L L
E N H E Q E Q D K E R
L T L L L W L L L L L
S I K T V S K N K K K
K K E A R R E E A Q N
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
Alignment Report of PAN2.meg ClustalW (Slow/Accurate, Gonnet) Tuesday, October 28, 2008 10:58
Page 11
X G T - - - - F D E V L N K L Y E H G R X T N W K V P E - - - - - - - - - - - - Majority 1610 1152 901 1088 934 1448 1324 1016 190 148 141 213
N M K N N D E I E K E
G G A K S G P G N G
T N I D E S K G T T K
E -
P -
E -
S T T A -
F F F F F W F F L F L
H R E E D N D Q R T D
K Q K R E D T A E S E
V K V V T I V K T T F
1620 L L L L L L L L L L L
K I N N N Q D L G Q V
G A S E K K K E R D A
L C V M I L L I V I L
Y Y Y Y Y Y L Y Y Y Y
E E E A Q D A R D T S
K T E W I L T H H L H
G G G G G G G G G G G
R R R K R R V K R R H
K K A A S I E Q K Q A
1630 M C H N L H T
D N N G S G S
W W F W F F W
K K K D K K Y
V A V P V P V
P P P M P P P
E T E A E A E
P T T P N G
1640
E T S L N A R
G Q T S P K N A A V HsPAN2 TriadPAN2 K G ScPAN2 Q Q Q G L G Q Q G L Chlamy PAN2 I E L Q S L P Q I P DdPAN2 R E H C P F H E Q Y MobPAN2 D S F S S P L L E P TbPAN2 MicpPAN2 T E W T I AurePAN2 T N W V NaegPAN2 N Q F K L G V A D K ThpPAN2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Majority 1650 1192 926 1115 969 1484 1361 1052 207 169 161 239
F S S V L A L
G S G V
S S A H
N I E T
G I R P
G D K T
G S D P
H L V P
A N K P
A F E F
G N A L
I F V D
1660
1670
1680
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 D L S I S V N Q L L D Q I K Q Q V S P T T T T T A T S S P DdPAN2 S A MobPAN2 V S R D G G S G N T A A S V T V P V A R G C TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
- - - - - - - - - - - - -
Majority
1690 1198 926 1115 979 1524 Q S P L K N Q N Q N K K K 1373 1084 207 169 161 239
HsPAN2 TriadPAN2 ScPAN2 Chlamy PAN2 DdPAN2 MobPAN2 TbPAN2 MicpPAN2 AurePAN2 NaegPAN2 ThpPAN2
Decoration 'Decoration #1': Shade (with solid black) residues that match the Consensus exactly.
