Binary vector pSiM24, complete sequence

Nucleotide

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Binary  vector  pSiM24,  complete  sequence GenBank:  KF032933.1 FASTA

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KF032933 7081 bp DNA circular SYN 02-SEP-2013 Binary vector pSiM24, complete sequence. KF032933 KF032933.1 GI:534467121 . Binary vector pSiM24 Binary vector pSiM24 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7081) AUTHORS Sahoo,D.K. and Maiti,I.B. TITLE A versatile gene expression vector pSiM24 for transient and stable expression of foreign genes in plants JOURNAL Unpublished REFERENCE 2 (bases 1 to 7081) AUTHORS Maiti,I.B. and Sahoo,D.K. TITLE Direct Submission JOURNAL Submitted (13-MAY-2013) KTRDC, Plant Genetic Engineering Research and Services, College of Agriculture, University of Kentucky, 1401 University Drive, Lexington, KY 40546, USA COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..7081 /organism="Binary vector pSiM24" /mol_type="other DNA" /db_xref="taxon:1392513" /country="USA" misc_feature complement(13..169) /note="left T-DNA border of Ti-plasmid; similar to INSD accession AF139061" misc_feature 170..234 /note="1st polylinker/multiple cloning site" promoter 235..860 /note="M24 promoter; derived from Mirabilis Mosaic Virus (MMV)" misc_feature 861..919 /note="2nd polylinker/multiple cloning site" polyA_signal 920..1559 /note="rbcSE9 terminator sequence; derived from pea" terminator complement(1572..1820) /note="nopaline synthase terminator; NosT; Nos-terminator" gene complement(1821..2902) /gene="nos-nptII" CDS complement(1821..2618) /gene="nos-nptII" /note="neomycin phosphotransferase II; KanR; codon-optimized for expression in plants; similar to INSD accession V00618" /codon_start=1 /transl_table=11 /product="kanamycin resistance protein" /protein_id="AGU26662.1" /db_xref="GI:534467123" /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2619..2902) /gene="nos-nptII" /note="Nos promoter; similar to INSD accession XV00087" misc_feature 2903..2956 /note="3rd linker/multiple cloning site" misc_feature complement(2957..3118) /note="right T-DNA border; similar to INSD accession

rep_origin

gene

3'UTR

CDS

promoter

rep_origin gene promoter CDS

3'UTR

J01826" complement(3137..3804) /note="ColE1 origin of replication; similar to INSD accession XM2327" complement(3805..4940) /gene="bla" /note="ampR" complement(3805..3951) /gene="bla" /note="similar to INSD accession XM2327" complement(3952..4812) /gene="bla" /note="confers resistance to ampicillin; similar to INSD accession XM2327" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AGU26661.1" /db_xref="GI:534467122" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" complement(4813..4940) /gene="bla" /note="similar to INSD accession XM2327" 4947..5570 /note="pRK2-Ori V; similar to INSD accession AF139061" 5577..7081 /gene="pRK2-trfA" 5577..5880 /gene="pRK2-trfA" 5881..7029 /gene="pRK2-trfA" /note="trans-acting replication; similar to INSD accession X00713" /codon_start=1 /transl_table=11 /product="pRK2-trfA" /protein_id="AGU26663.1" /db_xref="GI:534467124" /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVG SMVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKK RKTAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALF TTRNKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPI TFTFYELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLD RGKKTSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKL ETFRLMCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" 7030..7081 /gene="pRK2-trfA" /note="trfA-terminator"

ORIGIN 1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501

gcgcgcggta aatttgttta ctcggcacaa gaggcctgtg cacagacatc gggagccacc cttgtgtgaa tggtccctcc tgacgtctaa aagataataa ctaaaaaaga aagaaaatta caggacgaag ggaacacaaa ggaatcgaaa tcctgcagga tcaatgcatc ttgggaaaac cggttttcgc gtccttttgt aatttgaaat ggcctctaat tattcactag aagtatgtcc ccttgtcaga gtatgaaaat

cccagtacat caccacaata aatcaccact cgcatcccag aacatcttat actaaaacat gcatgtgaag actcacagct tccccccaac tgttgaagat tgatgcccga gtgaagcatc gaaatgacgt tcagaaggaa tctctcatct tcctctagag agtttcattg tgtttttctt tatcgaactg tcattctcaa tataagagat gaccgaagtt gcaacaaata tcttgtgttt ttctaatcat attttttaat

taaaaacgtc tatcctgcca cgatacaggc ggtgggtccc cgtcctttga tgctttgtca ccggtccctc caatcagtga ttcgtccaca aagagtggga cagccacttg ttccagtggt aagccatgac gagatcaatc aagcttggat ctttcgttcg cgcacacacc gtaccatttg tgaaatggaa attaatatta atgcaaacat aatatgagga tattttcaga tagacattta tgctttataa gcattttatg

cgcaatgtgt ccagccagcc agcccatcag gtataccccg agataagata aaagctaaaa cactaagaaa gcaacaggac gacatcaaca gccaccacta tgtgaagcat ccctccactc gtctaatccc gaaatcaaaa ccctcgaggt tatcatcggt agaatcctac ttgtgcttgt atggatggag tttgtttttt tttgttttga gtaaaacact cctagaaaag tgaactttcc ttatagttat acttgccaat

tattaagttg aacagctccc tccactagac gggaattcgt ataatgttga aagatgatgc attagtgaag gaaggaaatg tcttatcgtc aaacattgct gtgaagccgg acagctcaat acaagaattt tcggaatcga taacacgcgt ttcgacaacg tgagttcgag aatttactgt aagagttaat ctcttatttg gtaaaaatgt tgtagttgta ctgcaaatgt tttatgtaat actcatggat tgattgacaa

tctaagcgtc cgaccggcag cagtaccttg caacttcgtc agataagagt ccgacagcca catcttccag acgtaagcca ctttgaagat ttgtcaaaag tccctccact cagtgagcaa ccttatataa aatcaaaatc gtcgacgagc ttcgtcaagt tattatggca gttttttatt gaatgatatg ttgtgtgttg gtcaaatcgt ccattatgct tactgaatac tttccagaat ttgtagttga catgcatcaa

1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 4381 4441 4501 4561 4621 4681 4741 4801 4861 4921 4981 5041 5101 5161 5221 5281 5341 5401 5461 5521 5581 5641 5701 5761 5821 5881 5941 6001 6061 6121 6181 6241 6301

tcgatactag ctatattttg ccatctcata cagaaattat ttgccaaatg tgggaatctg agttcttcag agccttccgc caagcatcac gcaaaaagtt agtccagctt gggcaagtag ttctcagctg agccaatctc gtagcaagcc tcagtcttaa gagcatccaa ggacttccag aattggatac gctagctgat gcttagctca gtcagttcca ccttaattct attcgcgatt tttcacgccc cgccaatata tatcgcgcgc gttgctggcg aagtcagagg ctccctcgtg cccttcggga ggtcgttcgc cttatccggt agcagccact gaagtggtgg gaagccagtt tggtagcggt agaagatcct agggattttg atgaagtttt cttaatcagt actccccgtc aatgataccg cggaagggcc ttgttgccgg cattgctaca ttcccaacga cttcggtcct ggcagcactg tgagtactca ggcgtcaata aaaacgttct gtaacccact gtgagcaaaa ttgaatactc catgagcgga atttccccga ggcggccgtc ccgcggccgc ggcggacgtt ggctcgattt attttacgcg ttgacacttg gcagagtgct aaaatccagc acctgctttt gtgaccgcgc actacctaga atcaagtgtt gtattcgtgc gggaccgact aatcaggaat atgaatcgca gccgaagatg cagtcggtcg ctggccccgc caggaagccg aagaagcgca gccctgctga gccccgtggc

tctagtaaca ttttctatcg aataacgtca atgataatca tttgaacgat gagcagcaat caatatccct aatcaatgaa catgagtaac cagctggagc ccatcctagt caggatcaag gagcaaggtg ttccagcctc aagaaagtct cgaaaagaac tagtctgttg catgaagtcc cgaggggaat agtgacctta ttaaactcca aacgtaaaac ccgctcatga aattaacctg ttttaaatat tcctgtcaaa ggtacctgag tttttccata tggcgaaacc cgctctcctg agcgtggcgc tccaagctgg aactatcgtc ggtaacagga cctaactacg accttcggaa ggtttttttg ttgatctttt gtcatgagat aaatcaatct gaggcaccta gtgtagataa cgagaaccac gagcgcagaa gaagctagag ggcatcgtgg tcaaggcgag ccgatcgttg cataattctc accaagtcat cgggataata tcggggcgaa cgtgcaccca acaggaaggc atactcttcc tacatatttg aaagtgccac tatggccctg cggcgttgtg gacacttgag cggccggcga agtttcccac aggggcgcga gacagatgag atttgcaagg aaaccaatat acgccgaagg tgtggcgcaa ttggctctca agggcaagat tcattgccga aagggcacat ccttcgatcg ccgaaacgat gctcgatggt cggcccttcc ccggcctggc agaccgccgg agcataccaa cggataccat

tagatgacac cgtattaaat tgcattacat tcgcaagacc tcagaagaac tccgtaaagc agtagcgaga tccagaaaac aacaagatcc caatccttga tctagccctt agtgtgaagc tgaagaaagc agtaacaaca agcagcctca tggccttccc agcccaatcg atcttgctca ttatggaacg ggcgactttt gaaacccgcg ggcttgtccc tcagatctgg tggttggcat ccgattattc cactgatagt caaaaggcca ggctccgccc cgacaggact ttccgaccct tttctcatag gctgtgtgca ttgagtccaa ttagcagagc gctacactag aaagagttgg tttgcaagca ctacggggtc tatcaaaaag aaagtatata tctcagcgat ctacgatacg gctcaccggc gtggtcctgc taagtagttc tgtcacgctc ttacatgatc tcagaagtaa ttactgtcat tctgagaata ccgcgccaca aactctcaag actgatcttc aaaatgccgc tttttcaata aatgtattta gggccccgct caaacgcgcc gatacctcgc gggccgactc cgtggagctg agatgatgtg ctactgacag gggcgcacct gtttccgccc ttataaacct ggggtgcccc cgatgccggc ggccgaggcc tcggaatacc taaggtggat tgccccggcg caaggcgtat cgcctcgcgc ccagcaggcc ggccccgtcg caagtcgatg cgaggatctg gcagcagatc gcgggccatg

cgcgagcgat gtataattgc gttaattatt ggcaacagga tcatcgagga acaaggaatc gcaatatcct cttccgttct tcaccatctg tgttcctcat tcaattctgt cttctcatag aaatcttgtc tcaagaacag tcttgaagct tgagcagaaa tatccaaaaa attcccattt tcagtggagc gaacgcgcaa gctcagtggc gcgtcatcgg tgacccctat gcacatacaa taataaacgc ttaaactgaa gcaaaaggcc ccctgacgag ataaagatac gccgcttacc ctcacgctgt cgaacccccc cccggtaaga gaggtatgta aagaacagta tagctcttga gcagattacg tgacgctcag gatcttcacc tgagtaaact ctgtctattt ggagggctta tccagattta aactttatcc gccagttaat gtcgtttggt ccccatgttg gttggccgca gccatccgta gtgtatgcgg tagcagaact gatcttaccg agcatctttt aaaaaaggga ttattgaagc gaaaaataaa caccgggctg agaaacgccg ggaaaacttg acccggcgcg gccagcctcg gacaagcctg atgaggggcg attgacattt gtttttcggc tgtttttaac cccttctcga gacaagcagg cacggcaagt aagtacgaga tatctggaca tgagtcgggg cgccaggaac accgtcatgc accgccaaga gccgccgtcg accatcgaca gccaagcagg aaggaaatgc ccgaatgata

aatttatcct gggactctaa acatgcttaa ttcaatctta gcctgtagaa tatcagccca gatacctatc ccaccataat gcattctagc caagatcatc gcttagcttg catcagccat ctggaacctc cagcgcatgg cattaagagc gcctaaaaac gcctctcaac gagagtgaat atttttgaca taatggtttc tccttcaacg cgggggtcat gtaaggggcc atggacgaac tcttttctct ggcgggaaac aggaaccgta catcacaaaa caggcgtttc ggatacctgt aggtatctca gttcagcccg cacgacttat ggcggtgcta tttggtatct tccggcaaac cgcagaaaaa tggaacgaaa tagatccttt tggtctgaca cgttcatcca ccatctggcc tcagcaataa gcctccatcc agtttgcgca atggcttcat tgcaaaaaag gtgttatcac agatgctttt cgaccgagtt ttaaaagtgc ctgttgagat actttcacca ataagggcga atttatcagg caaatagggg gttgccctcg tcgaagccgt gccctcactg gcgttgacag caaatcggcg gggataagtg cgatccttga gaggggctgt caccgctaac cagggctgcg accctcccgg agcgcaccga atttgggcaa aggacggcca ccaaggcacc caatcccgca tgatcgacgc gtgccccgcg tcgaacgcga aacgctcgcg cccgcggcac tcagcgaagc agctgtcgct ccgcccgctc

agtttgcgag tcataaaaac cgtaattcaa agaaacttta agcaatcctt ctctccacca agcaactcca gtttggaagg cttgagccta ctgatccaca gtgatcaaat aatggaaacc tcccaaaaga aacaccagta accagaaaga agcagcatca ccaagcagca atgagactct agaaatattt tgacgtatgt ttgcggttct aacgtgactc ggccatttaa ggataaacct taggtttacc gacaatctga aaaaggccgc atcgacgctc cccctggaag ccgcctttct gttcggtgta accgctgcgc cgccactggc cagagttctt gcgctctgct aaaccaccgc aaggatctca actcacgtta taaattaaaa gttaccaatg tagttgcctg ccagtgctgc accagccagc agtctattaa acgttgttgc tcagctccgg cggttagctc tcatggttat ctgtgactgg gctcttgccc tcatcattgg ccagttcgat gcgtttctgg cacggaaatg gttattgtct ttccgcgcac ccgctgggct gtgcgagaca acagatgagg atgaggggca aaaacgcctg ccctgcggta cacttgaggg ccacaggcag ctgtctttta ccctgtgcgc cccgctgtcg cttcttccgc ggggtcgctg gacggtctac aggcgggtca aggagggtga cggcttctcg cgaaaccttc ttcggtccag ccggctggaa catgaccacc caagcaggcc gttcgacatc ggccctgttc

6361 6421 6481 6541 6601 6661 6721 6781 6841 6901 6961 7021 7081 //

accacccgca aacaaggatg gtctggcagc accttctatg gccgaagaat ggccatctgg acctcgcgct tataccaagt gattatttct tgcggctcgg gaactgcgcg aagcgctagg c

ataagaagat tcaagatcac aggtcctgga aactgtgcca gcctgtcgcg aatcggtcag gccaggtcct tcatctggga cgtcgcaccg attcgacccg gctcgggcct gccttgtggg

cccgcgcgaa ctataccggc atatgccaag ggatctgggc cctccaggcg cctgctgcac gatcgacgaa gaagtatcgc cgaaccgtat cgtcaagaag ggtcgaacat gtcagttccg

gccctccaga gtcgaactgc cgcaccccga tggtcgatca accgcgatgg cgcttccgcg gaaatcgtcg aagctgtcgc ccgctgaagc tggcgcgaac gcctgggtca gctgggggtt

ataaggtcat gggccgatga tcggcgaacc atggccgcta gcttcacctc tcctggatcg tcctgttcgc cgaccgcccg tggaaacctt aggtcggcga atgatgatct cagccagcgc

cttccacgtc tgatgaactg gatcaccttc ttataccaag ggatcgcgtc cggcaagaag cggcgatcat ccgcatgttc ccgcctgatg agcctgcgaa ggtccattgc tttacgatat