BLAST-based validation of metagenomic sequence assignments - PeerJ
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BLAST-based validation of metagenomic sequence assignments - PeerJ
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BLAST-based validation of metagenomic sequence assignments Adam L. Bazinet, Brian D. Ondov, Daniel D. Sommer, and Shashikala Ratnayake Supplementary Information
B. anthracis 0.100
sensitivity
0.075
Approach used to compute FP
0.050
near neighbour false negatives
0.025
0.000
250 bp
250 bp alternate
150 bp
CLE (N/A)
experiment 4VQQMFNFOUBSZ'JHVSF44FOTJUJWJUZGPSEFUFDUJPOPG#BOUISBDJTJTQMPUUFEBDSPTTGPVSFYQFSJNFOUTCQTJNVMBUFE SFBET CQTJNVMBUFESFBETVTJOHBOBMUFSOBUFSFQSFTFOUBUJWFHFOPNF CQTJNVMBUFESFBET BOEDMBEFMFWFMFYDMVTJPO $-& 5IFUXPapproaches used to computefalse positives OFBSOFJHICPVSBOEGBMTFOFHBUJWFT BSFBMTPDPNQBSFE
C. botulinum 1.00
sensitivity
0.75
Approach used to compute FP 0.50
near neighbour false negatives
0.25
0.00
250 bp
250 bp alternate
150 bp
CLE
experiment
4VQQMFNFOUBSZ'JHVSF44FOTJUJWJUZGPSEFUFDUJPOPG$CPUVMJOVNJTQMPUUFEBDSPTTGPVSFYQFSJNFOUTCQTJNVMBUFE SFBET CQTJNVMBUFESFBETVTJOHBOBMUFSOBUFSFQSFTFOUBUJWFHFOPNF CQTJNVMBUFESFBET BOEDMBEFMFWFMFYDMVTJPO $-& 5IFUXPapproaches used to computefalse positives OFBSOFJHICPVSBOEGBMTFOFHBUJWFT BSFBMTPDPNQBSFE
E. coli O157:H7 str. Sakai
sensitivity
0.003
Approach used to compute FP
0.002
near neighbour false negatives
0.001
0.000
250 bp
250 bp alternate (N/A)
150 bp
CLE (N/A)
experiment
4VQQMFNFOUBSZ'JHVSF44FOTJUJWJUZGPSEFUFDUJPOPG&DPMJ0)TUS4BLBJJTQMPUUFEBDSPTTGPVSFYQFSJNFOUT bQTJNVMBUFESFBET CQTJNVMBUFESFBETVTJOHBOBMUFSOBUFSFQSFTFOUBUJWFHFOPNF CQTJNVMBUFESFBET BOE DMBEFMFWFMFYDMVTJPO $-& 5IFUXPapproaches used to computefalse positives, "near neighbour" and "false negatives", are also compared.
Y. pestis 0.125
0.100
0.075
sensitivity
Approach used to compute FP near neighbour false negatives 0.050
0.025
0.000
250 bp
250 bp alternate
150 bp
CLE
experiment
4VQQMFNFOUBSZ'JHVSF44FOTJUJWJUZGPSEFUFDUJPOPG:QFTUJTJTQMPUUFEBDSPTTGPVSFYQFSJNFOUTCQTJNVMBUFESFBET CQTJNVMBUFESFBETVTJOHBOBMUFSOBUFSFQSFTFOUBUJWFHFOPNF CQTJNVMBUFESFBET BOEDMBEFMFWFMFYDMVTJPO $-& 5IFUXPapproaches used to compute false positives OFBSOFJHICPVSBOEGBMTFOFHBUJWFT BSFBMTPDPNQBSFE
