cells arranged in variable architecture with cribriform

S58

cells arranged in variable architecture with cribriform, trabecular and tubular patterns. The SBR grade was I. Tumour cells tested negative for estrogen receptors (ER-), progesterone receptors (PR-), and HER2/neu. None of the 6 axillary lymph nodes excised were positive for malignancy. Conclusion: ACC of the breast is a rare entity that should be differentiated from other types of invasive breast carcinomas; Although hormone receptors and HER2 are typically negative in ACC of the breast, it is defined as a carcinoma of low malignant potential by the WHO classification.

PS-01-067 Evaluation of Ki67 expression in breast cancer (pT1-2N0) using tissue microarrays and image analysis A. Kudaybergenova*, R. Paltuev * St. Petersburg, Russia Objective: Ki67 index in breast cancer seems to be strongly prognostic and according last St. Gallen recommendation can predict response to chemotherapy. However, there is no consensus on counting areas or cut-off values for patient stratification. Method: We examined a retrospective series of breast cancer pT1-2N0 treated within 2000–2009 years (n = 733) using TMA. After whole slide scanning TMA blocks was constructed using 2 mm punches and TMA master device (3DHistech, Budapest). The percentage of Ki67 positive nuclei was evaluated by image analysis within each core. Results: Mean of tumour cells in breast carcinoma in 1 mm2 of histology slide was 6828,981 + %%- 207,5860 cells, median—6413,727 cells. Mean of Ki67 positive cells in 1 mm2 was 1360,73+/−92,91 in absolute numbers of cells. Ki67 index = 19,92 %+/−1,36. Conclusion: For breast cancer TMAs (with big diameter core) are an efficient and reliable alternative to WS IHC staining for Ki67. This technique allow reduce time, reagent and finally cost of each type of analysis. Using image analysis for evaluation of Ki67 index is essential.

PS-01-069 Benign hepatocellular nodule as regression of a breast cancer liver metastasis: Case report D. Vinha Pereira*, P. Chaves, S. André * IPO Lisboa Francisco Gentil, Dept. of Anatomical Pathology, Lisbon, Portugal Objective: The surgical excision of breast cancer liver metastasis (BCLM) is a relative uncommon procedure. Consequently, the report of the histological features of the regression of BCLM after neoadjuvant chemotherapy (NACh) is almost unknown. Method: We report the case of a patient with BCLM confirmed by biopsy with histological complete regression after NACh with features of a regenerative hepatic nodule. Results: A 43 years-old woman was diagnosed with a cT2 N0, G3, breast invasive carcinoma, estrogen receptors positive (100 %) progesterone receptors negative, ERBB2 3+, with a synchronous liver metastasis with 16 mm by ultrasonography, confirmed by biopsy. After NACh, the liver metastasis reduced to 10 mm by ultrasonography, and the patient underwent hepatectomy and breast lumpectomy. Histologically, a breast tumour bed with 6 mm with 5 % of overall cancer cellularity, and a 10 mm hepatocellular nodule with steatohepatitis and regenerative features, were found. Posterior radiologic evaluation of the liver confirmed the surgical excision of the metastatic nodule. The patient is alive and well 6 months after surgery. Conclusion: We illustrate a regenerative hepatic nodule as a form of total BCLM regression. The histologic re-evaluation of BCLM after NACh may be as important as the re-evaluation of the primary tumour to appraise the degree of regression.

Virchows Arch (2016) 469 (Suppl 1):S1–S346

PS-01-070 Breast cancer incidence, demographic, and pathological characteristics in Bosnia and Herzegovina F. Skenderi*, N. Ibisevic, J. Topcagic, N. Bilalovic, S. Vranic * Clinical Center Sarajevo, Dept. of Pathology, Bosnia and Herzegovina Objective: Breast cancer is a leading cause of cancer morbidity and mortality in women in less developed countries. No epidemiological, pathological, and clinical data for breast cancer in Bosnia and Herzegovina have been published so far. In addition, there is no screening program for breast cancer in our country. We present 10 years data from a tertiary level institution covering a representative population of Bosnia and Herzegovina. Method: Medical records from the Department of Pathology, dating from 2005 to 2015, were searched for breast cancer cases. Demographic, pathological, and molecular characteristics of the cases were recorded. Epidemiological data were approximated based on the population number covered by our institution. Results: There were 296 new cases per year, per 438 000 population covered (crude incidence rate 68/100 000 persons/per 10 years). The average age of the patients was 58.4 years (range, 26–99 years). The most affected age group was 51–60 years (28 %), followed by age 61–70 (24 %), 41–50 (22 %), while 7 % of the affected women were less than 40 years old. The most frequent histotype was invasive ductal carcinoma NOS (70 %), followed by lobular carcinoma (10 %), mixed type carcinoma (5 %), and all special types (15 %). The majority of the tumours were Nottingham histologic grade 2 (47 %), followed by grade 3 (40 %) and grade 1 (13 %). The most frequent tumour stage was pT2 (53 %), followed by pT1 (27 %), pT4 (11 %), and pT3 (9 %). Her-2/neu overexpression was observed in 15 % of the cases. The most common molecular type was Luminal A (74 %), followed by triple negative cancer (11 %), Luminal B (8 %), and Her2 enriched type (7 %). Conclusion: Higher pT stage at initial presentation is due to lack of effective screening for breast cancer in Bosnia and Herzegovina. Other histopathologic and molecular characteristics from our cohort are in line with the published data.

PS-01-071 Decreased immunoexpression of vav2 could be a potential marker in breast carcinoma progression H.-W. Hwang*, S.-Y. Cho, E.-Y. Cho * Samsung Medical Center, Dept. of Pathology, Seoul, Republic of Korea Objective: The oncoprotein Vav2 has been demonstrated to be expressed in human breast cancer cells, however its association with the clinicopathologic parameters and clinical outcome remains uncertain. Method: We investigated Vav2 expression by immunohistochemistry in tumour tissue from 250 breast cancer patients who underwent curative resection in Samsung medical center. The median H-score was used to evaluate Vav2 expression, and the score was calculated separately in ductal carcinoma in situ and invasive carcinoma portion. The association of Vav2 expression with clinicopathologic variables, such as molecular phenotype (ER, PR and HER2 status), tumour size, nuclear grade, histologic grade, pN stage, overall survival, and recurrence free survival was analyzed. Results: The Vav2 was strongly expressed in all normal breast duct and ductal carcinoma in situ. Invasive carcinoma showed significantly decreased expression in comparison with DCIS(p =