Detection by using HPLC technique of Chloro-cypermethrin and Chlorpyrifos concentration residual in plasma of slaughtered animals (cattle, sheep, gaut, and camel) in najaf city Mohammad Taha. Naqi*
Nadia K. J. Al-Dawah*
Hala Baqer Thanoon** *Department Of Physiology & Pharmacology, Faculty Of Veterinary Medicine, University of Kufa , Iraq **Department Of Physiology, Biochemistry & Pharmacology, Faculty Of Veterinary Medicine, University of Mosul , Iraq E-mail:
[email protected] [email protected] Abstract Determination of Chloro-cypermethrin and Chlorpyrifos
insecticides residual
concentration in plasma in animals (cattle, sheep, gout, and camel) that slaughter in najaf city slaughter house . the results show presence of Chloro-cypermethrin significantly at conc. (0.33± 0.003 µg mL-1) in the plasma than other animal species by using HPLC technique that revel that cattle more exposed to Chloro-cypermethrin and Chlorpyrifos more than other species. Key words: Chloro-cypermethrin, Chlorpyrifos, HPLC, plasma, acetonitrile
، عن متبقيات الكلورو – سابيرمثرين و الكلوروباريفوس في دم (الماشيةHPLC الكشف باستخدام تقنية و الجمال) المذبوحة في مجزرة مدينة النجف االشرف،الماعز،االغنام *نادية كاظم جاسم الدواح
* محمد طه نقي **حال باقر ذنون 1
العراق/ جامعة الكوفة/ كلية الطب البيطري/ *فرع الفسلجة و االدوية العراق/ جامعة الكوفة/ كلية الطب البيطري/ **فرع الفسلجة والكيمياء الحياتية و االدوية
[email protected] [email protected] الخالصة الماعز، االغنام، تم تحديد تركيزمتبقيات الكلورو – سابيرمثرين و الكلوروباريفوس في بالزما دم الحيوانات ( الماشية و الجمال) المذبوحة في مجزرة مدنية النجف االشرف حيث اظهرت النتائج ارتفاع معنوي بمتبقيات السايبرمثرين في، ) ممن باقي االنواع المذبوحة حيث يظهر لنا تعرضها للمبيد1- مايكروغرام مل0.33± 0.003( بالزما الماشية بتركيز . اكثر من غيرها من حيوانات المزرعة اسيتونيترال، بالزما، HPLC ، الكلوروباريفوس, الكلورو – سابيرمثرين: الكلمات المفتاحية Introduction Chloro-cypermethrin insecticide widely used to control pests of soil, plants and animals [1]. The widespread agricultural and animal applications of chloro-cypermethrin pesticides are attributed to their low environmental persistence [2]. Despite their low persistence, exhibit toxicity to humans and animals and the presence of residues of such compounds in our food supply has raised the safety issue. chloro-cypermethrin and Chlorpyrifos is a type II pyrethroid insecticide used in domestic animal infested by different arthropod parasites like mites, ticks, bots and grubs which causes economic loss of livestock producers. Now-a-days, a chlorocypermethrin is extensively used in agricultural field for crop production and contaminated crops may cause damage to human beings due to consumption resulting possible health hazards. But literature on adverse effect caused by combination of these two insecticides in mice is scarcely available. Therefore the present work was undertaken to explore the effect of chlorocypermethrin on biochemical parameters and residual level in different tissues of farm animals[3]. Materials and Methods The samples collected from 20 adult healthy animals, included four types of ruminants (sheep, cattle, goat, and camel) in Najaf slaughter house. A blood samples were collected using 2
EDTA's (AFCO, Jordan) test tubes. Plasma was separated from blood by centrifugation at 3,000 rpm (Hettich, Germany) for 15 min. Separated plasma used for measurement of chlorocypermethrin concentration by HPLC. Analysis of Chloro-cypermethrin and Chlorpyrifos Collected (0.5 ml) o f p l a s m a was added to 2 ml of acetonitrile (HPLC grade).Mixture was homogenized thoroughly and centrifuged at 2000 rpm for 10 min. The supernatant was collected and 2 ml of acetonitrile added again and the procedure repeated twice. Whole collected supernatant was reduced in volume using rotary vacuum evaporator and reconstituted with 0.5 ml of methanol (HPLC grade) and subjected to membrane filtration and injected 20 µl of sample to the HPLC injection port. Retention time of the drug was 2.45 min[3]. Instrument and Chromatographic Condition Chloro-cypermethrin and Chlorpyrifos in blood and tissues were analysed on a HPLC system (SHIMADZU, SPD – M 10 A, JAPAN) fitted with binary pump (LC-20AT), diode array detector, sampler and data station. A 5 µ Luna Phenomenox (250 x 4.6 mm) C 18 (2)HPLC column was used. The mobile phase consisted of acetonitrile and water with a ratio of 50: 50 (V/V). The flow rate of mobile phase was 1.0 ml min-1 and the eluent was monitored with a diode array detector adjusted wave length at 273 nm. The chromatograms were integrated on a data station[3]. Statistics When applicable the data were subjected to analysis of variance followed by the least significance difference test , multiple t-test (ANOVA) [4]. The level of significance was at p,
