range of 103â20690 pg/mL with the analysis time of 1.5 min. The method is fully ..... peak at the retention time of naratriptan and IS indicates ion enhancement.
Mar 13, 2018 - RESEARCH ARTICLE. Development and application of a simple, rapid and sensitive method for detecting moderately carbendazim-resistant.
graine drugs rizatriptan, zolmitriptan, naratriptan, and suma- triptan in human serum by LCâESI-MSâMS. The drugs were extracted by solid phase extraction on ...
Feb 5, 1992 - Harenberg J. Giese C. Kncidler A,. Zimmermann R: Comparative study on ... Broze Jr GJ, Warren LA, Novotny. WF, Higuchi DA, Girard JJ, Mile-.
Feb 7, 2013 - [email protected] (Z.C.) .... An Agilent Eclipse XDB-C18 column ... Table 1. HPLC-MS/MS mobile phase gradient elution condition.
14 Jul 2016 - Immunochromatographic Test Kit as a Diagnostic. Strategy ... The modified Canadian Triage & Acuity Scale (CTAS) was applied as the triage.
HIV (The Merck Index 1996). STV is converted in- tracellularly to triphosphate which stops the DNA syn- thesis of retroviruses through competitive inhibition of.
health (Chan et al., 2010; Zong et al., 2011; Kra¨mer, 1998;. Viguier and Hulme, 2006). However, unregulated copper can lead to disturbance of the cellular ...
2013 Production and hosting by Elsevier B.V. on behalf of King Saud University. 1. ... is advantageous because it is easily operated and inexpensive. A.
Methods: ATV and RST were analyzed on a BDS hypersil C18 column (250 ... Keywords: Atorvastatin, Rosuvastatin, Statin, RP- HPLC and validation, Plasma.
Apr 21, 2008 - and occurs when an incident beam in energy is close to an absorption ... 2.2. Reagents. A stock solution containing 355.00ngmLâ1 of Cl. â was.
in MeOH to make individual stock solutions of 1.0 mg mLâ1. These solutions .... nazepam are 1.5 and 10.5 [15], for 7-aminoclonazepam, however, they were not ... oxazepam, bromazepam, temazepam, midazolam, alprazo- lam, flunitrazepam ...
Mar 2, 1988 - phase columns has been developed to separate retinol and ... separation of retinol from retinyl esters by using a C18 column and an HPLC ...
phenolic antioxidant addition since BHA and BHT have also been found to be toxic at higher levels.5,6. Analytical methods for determining antioxidants include.
Thermo Scientific Dionex ICS-3000 or Dionex ... Chromeleon⢠software tracks the amount of KOH used ... application, electrolytic eluent generation delivers.
and HLA-Cw7. We conclude that the nano-LC MALDI. MS/MS approach is a sensitive tool for the rapid and automated identification of MHC-associated tumor ...
Aug 21, 2011 - trimethyl-1,4-dihydropyrimidine-2-thiol: Analysis of Real Samples. Ganesh S. Kamble,. â ..... nitrate, nitrite, EDTA, citrate. 25. Bi(III), Te(IV), Al(III) ...
27, October 2003. Development of a Rapid and Sensitive Method for the ..... were controlled using MassLynx NT 3.5 software (Waters). An injection volume of 10 ...
Jul 5, 2012 - raloxifene were tested for selection as internal standards in optimized chromatographic conditions. Peaks of acyclovir and diclofenac showed.
Conclusion: The HPLC coupled with C30 column efficiently resolved fifteen carotenoids and their isomers in shorter runtime of 20 min. Application of this method ...
3,4-methylenedioxyethylamphetamine, amphetamine, methamphetamine, and ephedrine in a single chromatographic run. Limits of detection of 2 pg/L or better ...
(LC-APCI-MS) method for identification and quantitation of several drugs, including amphetamine and methamphetamine and their methylenedioxy derivatives, ...
glutathione to active electrophilic species. Cytosolic GSTs occur as dimeric globular proteins composed of subunits with relative molecular masses of 23â29 kDa ...
Clinical Chemistry 47:5 867– 873 (2001)
Enzymes and Protein Markers
Development of a Rapid and Sensitive Immunofluorometric Assay for Glutathione S-Transferase A Laila K. Dajani,* Elisabeth Paus, and David J. Warren Background: The short half-life of the ␣-class glutathione S-transferases (GSTAs) in plasma combined with their even distribution throughout the liver lobule suggests that they may be useful complements to the more traditionally used liver markers. However, the currently available assays for measuring GSTAs in biological fluids have a poor dynamic range and are cumbersome, requiring multiple steps and prolonged incubation times. Methods: Hybridomas that secrete monoclonal antibodies to human GSTAs were produced and used to develop a rapid one-step immunometric assay for the determination of GSTA in serum. The assay uses a time-resolved immunofluorometric assay (TR-IFMA) format and requires 35 min of incubation. The reference interval was determined using 208 serum samples from healthy blood donors. We also compared our TR-IFMA with a commercially available enzyme immunoassay (EIA) for GSTAs. Results: The assay had a detection limit of 0.07 g/L with a measuring range up to 625 g/L. Within-run imprecision (CV) was 1.8 –2.6% over the concentrations of GSTA tested (2.5–311 g/L), with a between-run CV of
