and Waterman,. 1983) and lapine .... 1000. IU of sodium heparin and. 20,000. IU of potassium penicillin. G per ml. After each .... by Dr. Thomas. G. Kennedy,.
BIOLOGY
OF
REPRODUCTION
30,936-944(1984)
Concentrations
of Prostaglandins
in the Utero-ovarian
Venous J. SILVIA,
W.
E2,
Plasma
F2
and 6-Keto-Prostaglandin
of Nonpregnant
J. S. OTTOBRE
Division
of Animal West
and
and
Early
F1
Pregnant
Ewes1
E. K. INSKEEP2
Veterinary
Virginia
Morgantown,
and
Sciences
University
West
Virginia
26506
ABSTRACT The
F10
effect
of
pregnancy
on
concentrations
of prostaglandins
E2,
F2
and
6-keto-prostaglandmn
(PGE2,
PGF20 and 6-keto-PGF10) in utero-ovarian venous plasma was examined in ewes on Days 10 through 14 after estrus, an interval which includes the critical period for maternal recognition of pregnancy. The utero-ovarian vein ipsilateral to a corpus luteum was catheterized on Day 9 or 10 in 6 pregnant and 8 nonpregnant ewes. Five blood samples were collected at 30-mm intervals for 2 h beginning at 0500 and 1700 h daily. Sampling began at 0500 h on the day after catheterization. The mean and variance within each 2-h collection period were calculated for each ewe. The natural logarithm of the variance in each collection period (In variance) was used as an estimate of the fluctuations in secretory activity by the endometrial-conceptus complex. Patterns of the mean concentrationsofPGE3 weredifferentbetween pregnant PGE2 being higher in the pregnant ewes beginning on Day 13. There In variance in PGE2 to differ (P
