F-119. PATIENTS WITH BARRETT'S OESOPHAGUS AND ADENOCARCINOMA OF THE. OESOPHAGUS AND OESOPHAGOGASTRIC JUNCTION HAVE ...
Interactive CardioVascular and Thoracic Surgery
Abstracts 23rd European Conference on General Thoracic Surgery 31 May–3 June 2015, Lisbon, Portugal F-119 PATIENTS WITH BARRETT’S OESOPHAGUS AND ADENOCARCINOMA OF THE OESOPHAGUS AND OESOPHAGOGASTRIC JUNCTION HAVE INCREASED OXIDATIVE STRESS ALSO IN PROXIMAL STOMACH Juha Kauppi1 , J. Rasanen1, E. Sihvo1, M. Ahotupa2, J. Salo1 1 Department of General Thoracic and Oesophageal Surgery Clinic, Heart and Lung Centre, Helsinki University Central Hospital, Helsinki, Finland; 2 Department of Biosciences, Turku University, Turku, Finland Objectives: Oxidative stress (OS) is an essential element in the pathogenesis of several cancers, including Barrett’s oesophagus (BO) and its transformation to oesophageal adenocarcinoma (OAC). The state of oxidative stress in proximal stomach of patients with BO and OAC is unknown. Isoprostanes are a very specific marker of OS and not previously used in determining OS from BO/OAC tissue samples. Methods: OS was measured from 42 patients having BO (n = 9), OAC (n = 9,) and both (n = 24). The control group included 15 patients with healthy gastric
and oesophageal mucosa. Biopsies were taken 5 cm below and above the squamo-columnar junction, and from obvious area tumour. 8-Isoprostanes (8-IP) were determined by STAT-8-Isoprostane EIA Kit. Glutathione reduced form (GSH) and glutathione oxidized form (GSSG) were measured by Glutathione Assay Kit (Cayman Chemical, Ann Arbor, MI, USA). 8-OH-deoxyguanosine was measured by the OxiSelect™ Oxidative DNA Damage ELISA Kit (8-OHdG) (Cell Biolabs Inc., San Diego, CA, USA). Results: The content of 8-IP (P = 0.039) and 8-OHdG (P = 0.008) were higher and GSH lower (P = 0.031) in proximal stomach of the study group as compared to controls. Helicobacter pylori infection was present in 8% of study patient. Conclusions: In proximal stomach of BO and OAC patients oxidative stress is elevated and antioxidative capacity reduced. This finding suggests that gastrooesophageal reflux causing BO also induces oxidative stress in proximal stomach, and may contribute to development of cancer of proximal stomach and gastric cardia. Disclosure: No significant relationships.
