LmjF.07.0510. 60S ribosomal protein L7a. 0.50. 2.346E8 29.7. LmjF.35.0420. 40S ribosomal protein S3A,. 0.45. 1.978E8 30.0. LmjF.32.3130 ribosomal protein ...
Supplemental Table 4. The complete list of proteins pulled down by tagged LeishIF4G3.The table shows the proteins that were pulled down with the SBP-tagged LeishIF4G3 and further analyzed by LC-MS/MS. The relative abundance of the proteins is represented by peptide peak areas and relative PAF values. The latter were determined by normalizing the peptide count of each protein to its molecular weight. The relative PAF was calculated for each protein by normalization to the PAF value of the bait protein. Proteins represented by less than two peptides were not included. All values were subjected to background substraction using the peptide count from a mock purification with a cell line expressing the tagged luciferase protein
Accession Number
Protein
Relative PAF
Area
Mol.Wt
Initiation factors LmjF.16.1600 LmjF.30.0450 LmjF.01.0770
eukaryotic translation initiation factor 4 gamma, eIF4G3 eukaryotic translation initiation factor 4e, eIF4E4 eukaryotic initiation factor 4a,
1.00 0.57 0.47
1.010E9 71.2 3.826E8 33.8 4.722E8 45.3
LmjF.17.0010 LmjF.17.1290 LmjF.36.6980 LmjF.30.3040 LmjF.28.2310 LmjF.25.1610 LmjF.34.2700 LmjF.07.0640 LmjF.36.3880 LmjF.32.2180 LmjF.36.0250
eukaryotic translation initiation factor 3a eukaryotic translation initiation factor 3b eukaryotic translation initiation factor 3c eukaryotic translation initiation factor 3d eukaryotic translation initiation factor 3e eukaryotic translation initiation factor 3f eukaryotic translation initiation factor 3g eukaryotic translation initiation factor 3h eukaryotic translation initiation factor 3i eukaryotic translation initiation factor 3k eukaryotic translation initiation factor 3l
0.23 0.26 0.28 0.09 0.25 0.09 0.11 0.18 0.10 0.13 0.14
1.961E7 1.663E7 3.118E7 1.263E7 2.612E7 2.522E7 1.037E7 1.999E7 2.130E7 1.236E7 1.321E7
87.6 80.7 82.0 60.6 46.4 36.7 28.8 37.9 45.4 26.3 72.6
LmjF.03.0980 LmjF.08.0550 LmjF.09.1070 LmjF.33.2740 LmjF.34.0350 LmjF.35.5040 LmjF.35.4130 LmjF.36.0890
initiation factor 2 alpha subunit translation initiation factor2 beta subunit eukaryotic translation initiation factor 2 gamma subunit, translation initiation factor IF-2, eukaryotic translation initiation factor 5 polyadenylate-binding protein 1 poly(A)-binding protein 2 eukaryotic translation initiation factor 6 (eIF-6)
0.15 0.20 0.26 0.06 0.09 0.21 0.20 0.24
2.431E7 1.843E7 1.467E7 1.045E7 8.056E6 9.742E6 2.853E7 1.299E7
49.0 38.0 52.6 92.3 42.9 62.6 65.3 27.1
elongation factor 2 elongation factor 1-alpha translation elongation factor 1-beta elongation factor-1 gamma elongation factor Tu, translation elongation factor 1-beta
0.39 0.26 0.15 0.14 0.11 0.11
1.769E8 1.198E9 7.242E7 7.780E7 1.398E7 6.068E7
94.1 49.1 25.6 46.2 51.5 23.3
60S ribosomal protein L7a 40S ribosomal protein S3A, ribosomal protein L3, 40S ribosomal protein S4, 40S ribosomal protein S2 ribosomal protein L1a, 40S ribosomal protein S6, 40S ribosomal protein SA, 60S ribosomal protein L2 60S ribosomal protein L13a 60S ribosomal protein L5 60S ribosomal protein L13 40S ribosomal protein S8, 60S ribosomal protein L10
0.50 0.45 0.45 0.44 0.38 0.33 0.32 0.31 0.30 0.28 0.27 0.23 0.23 0.18
2.346E8 1.978E8 1.027E8 1.067E8 6.409E7 1.061E8 6.481E7 2.029E8 8.569E7 4.850E7 1.442E8 2.190E8 2.923E7 3.138E7
29.7 30.0 47.5 30.7 28.6 41.0 28.3 27.5 28.3 25.4 34.0 24.7 24.9 24.5
Elongation factors LmjF.36.0180 LmjF.17.0081 LmjF.34.0840 LmjF.09.0970 LmjF.18.0740 LmjF.36.1430
Ribosomal proteins LmjF.07.0510 LmjF.35.0420 LmjF.32.3130 LmjF.13.1230 LmjF.32.0450 LmjF.29.1090 LmjF.21.1780 LmjF.36.5010 LmjF.32.3900 LmjF.15.0200 LmjF.35.1880 LmjF.29.2460 LmjF.24.2080 LmjF.04.0750
LmjF.20.1650 LmjF.35.1910
ribosomal protein S11 homolog ribosomal protein L15,
0.16 0.11
2.325E6 16.3 2.742E6 24.0
glutaminyl-tRNA synthetase glutamyl-tRNA synthetase arginyl-tRNA synthetase seryl-tRNA synthetase lysyl-tRNA synthetase tyrosyl or methionyl-tRNA synthetase-like protein threonyl-tRNA synthetase glycyl tRNA synthetase methionyl-tRNA synthetase prolyl-tRNA synthetase asparaginyl-tRNA synthetase cysteinyl-tRNA synthetase
0.11 0.10 0.31 0.23 0.20 0.20 0.19 0.15 0.11 0.10 0.09 0.09
7.769E6 4.551E6 1.609E7 2.275E7 1.205E7 7.812E6 1.225E7 1.092E7 6.022E6 1.066E7 5.290E6 7.447E6
66.0 67.6 78.2 53.1 67.0 19.6 89.4 70.3 83.8 81.5 99.4 88.5
T-complex protein 1, beta subunit 14-3-3 protein T-complex protein 1, gamma subunit chaperonin TCP20 14-3-3 protein-like protein heat shock protein T-complex protein 1, delta subunit heat shock 70-related protein 1, mitochondrial precursor chaperonin HSP60, mitochondrial precursor chaperonin containing t-complex protein chaperonin alpha subunit stress-induced protein sti1 glucose-regulated protein 78 heat shock protein 70-related protein
0.42 0.38 0.38 0.35 0.35 0.31 0.30 0.27 0.27 0.26 0.24 0.23 0.20 0.18
3.448E7 2.987E7 1.940E7 1.381E7 6.466E7 1.470E7 1.417E7 1.760E8 1.926E8 1.169E7 3.691E7 1.329E7 1.300E8 5.183E7
57.8 29.1 60.3 58.9 29.7 72.0 59.7 70.6 59.3 59.2 59.1 62.1 71.9 70.5
tRNA synthetase LmjF.15.1440 LmjF.30.3240 LmjF.27.1310 LmjF.11.0100 LmjF.15.0230 LmjF.16.1130 LmjF.35.1410 LmjF.36.3840 LmjF.21.0810 LmjF.18.1210 LmjF.34.2340 LmjF.12.0250
Molecular chaperons LmjF.27.1260 LmjF.11.0350 LmjF.23.1220 LmjF.13.1660 LmjF.36.3210 LmjF.33.2390 LmjF.21.1090 LmjF.30.2550 LmjF.36.2030 LmjF.32.1000 LmjF.32.3270 LmjF.08.1110 LmjF.28.1200 LmjF.26.1240
LmjF.26.0620 LmjF.18.1370 LmjF.36.6910 LmjF.33.0355 LmjF.36.6940 LmjF.36.0070 LmjF.04.0770 LmjF.35.3860
10 kDa heat shock protein heat shock protein T-complex protein 1, theta subunit, heat shock protein 83-1 protein disulfide isomerase stress-inducible protein STI1 homolog nascent polypeptide associated complex subunit- like protein, copy 1 T-complex protein 1, eta subunit
0.18 0.18 0.18 0.18 0.16 0.16 0.14 0.12
7.209E6 5.246E7 3.060E7 5.036E8 1.222E8 1.096E7 4.876E6 2.438E7
10.7 91.7 58.1 80.5 52.3 29.0 18.2 61.7
RNA binding protein RNA-binding protein RNA-binding protein mitochondrial RNA binding protein mitochondrial RNA binding protein 2,MRP2, gBP25 nucleolar RNA-binding protein la RNA binding protein
0.28 0.23 0.19 0.16 0.14 0.12 0.10
1.711E7 1.013E7 1.106E7 8.010E6 4.542E6 1.110E7 1.776E8
25.2 30.2 34.5 39.7 26.8 37.5 37.2
RNA helicase ATP-dependent RNA helicase ATP-dependent DNA helicase, ,ruvb DNA helicase-like protein ruvb-like 1 DNA helicase ATP-dependent RNA helicase ATP-dependent DEAD/H RNA helicase
0.47 0.42 0.36 0.33 0.14 0.14
3.982E7 3.234E7 2.086E7 1.295E7 1.500E7 1.077E7
49.5 67.0 53.6 50.3 100.2 64.0
actin alpha tubulin beta tubulin
0.34 0.33 0.23
1.109E8 42 9.866E7 49.7 7.598E7 49.7
RNA binding proteins LmjF.32.0750 LmjF.35.2200 LmjF.04.1170 LmjF.23.0760 LmjF.09.1120 LmjF.07.0990 LmjF.21.0540
RNA/DNA helicases LmjF.21.1552 LmjF.32.0400 LmjF.34.2610 LmjF.34.3500 LmjF.35.3100 LmjF.07.0340
Cytoskelton LmjF.04.1230 LmjF.13.0390 LmjF.33.0808
Protein degradation machinery LmjF.36.1600 LmjF.21.1830 LmjF.35.1380 LmjF.02.0370 LmjF.11.0240 LmjF.34.4370 LmjF.33.1610 LmjF.26.1570 LmjF.36.4360 LmjF.27.1460 LmjF.27.2660 LmjF.19.1120 LmjF.21.0760
proteasome alpha 1 subunit proteasome alpha 5 subunit, ,20S proteasome subunit alpha 5 mitochondrial processing peptidase, beta subunit proteasome regulatory non-ATPase subunit 6 proteasome alpha 7 subunit 20s proteasome beta 7 subunit peptidase M20/M25/M40 thimet oligopeptidase, ,metallo-peptidase, Clan MA(E), Family M3 proteasome regulatory ATPase subunit proteasome regulatory non-ATP-ase subunit 3 peptidyl dipeptidase, ,metallo-peptidase, Clan MA(E), Family M3 proteasome regulatory non-ATP-ase subunit proteasome regulatory non-ATP-ase subunit 5, ,19S proteasome regulatory subunit 4
0.24 0.21 0.20 0.16 0.14 0.13 0.13 0.12 0.11 0.10 0.10 0.10
1.945E7 1.704E7 2.510E7 1.008E7 8.929E6 6.880E6 2.253E7 1.520E7 1.447E7 6.522E6 8.814E6 9.224E6
29.6 26.8 54.5 58.8 27.8 24.7 51.5 77.1 45.6 37.9 76.5 46.5
0.10
6.585E6 54.2
activated protein kinase c receptor (LACK) small glutamine-rich tetratricopeptide repeat protein protein kinase, ,mitogen-activated protein kinase, protein phosphatase GTP binding protein, protein kinase A regulatory subunit GTP-binding protein mitogen-activated protein kinase 3, ,map kinase 3 mitogen-activated protein kinase, ,map kinase-like protein protein tyrosine phosphatase cell division protein kinase 2,cdc2-related kinase
0.53 0.24 0.21 0.17 0.13 0.13 0.12 0.09 0.08 0.08 0.08
8.252E7 1.703E7 1.043E7 1.003E7 3.171E7 1.039E7 4.127E6 9.120E6 6.950E6 3.021E6 3.475E6
enolase
0.45
1.558E8 46.1
Signal transduction LmjF.28.2750 LmjF.30.2740 LmjF.19.0150 LmjF.25.0750 LmjF.27.2330 LmjF.13.0160 LmjF.25.1420 LmjF.10.0490 LmjF.10.0200 LmjF.05.0280 LmjF.21.1080
34.4 45.7 30.9 44.9 44.0 56.2 24.2 43.8 46.3 25.2 34.4
Metabolic enzymes LmjF.14.1160
LmjF.25.2130 LmjF.10.0290 LmjF.01.0050 LmjF.36.2950 LmjF.34.3670 LmjF.36.3910 LmjF.21.0240 LmjF.32.3310 LmjF.28.2420 LmjF.24.0770 LmjF.36.1260 LmjF.35.0820 LmjF.33.2540 LmjF.30.2970 LmjF.27.1805 LmjF.29.0760 LmjF.31.2150 LmjF.35.3230 LmjF.23.1480 LmjF.35.1480 LmjF.19.0160 LmjF.28.2430 LmjF.36.1370 LmjF.35.3700 LmjF.36.3100 LmjF.28.0490 LmjF.31.1630 LmjF.19.0200 LmjF.23.0540 LmjF.16.0550 LmjF.19.0710 LmjF.25.2010 LmjF.35.0030 LmjF.25.1170 LmjF.18.0510
succinyl-CoA synthetase alpha subunit 0.40 isocitrate dehydrogenase [NADP], mitochondrial precursor, 0.39 carboxylase, 0.39 succinyl-CoA ligase [GDP-forming] beta-chain 0.32 vacuolar ATP synthase catalytic subunit A 0.31 S-adenosylhomocysteine hydrolase 0.30 hexokinase, 0.30 dihydrolipoamide dehydrogenase 0.28 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransfera0.26 malic enzyme, 0.25 fructose-1,6-bisphosphate aldolase 0.25 aspartate aminotransferase, 0.25 carboxypeptidase, ,metallo-peptidase, Clan MA(E), Family M32 0.25 glyceraldehyde 3-phosphate dehydrogenase, glycosomal 0.25 glycosomal phosphoenolpyruvate carboxykinase, 0.23 lipophosphoglycan biosynthetic protein, ,heat shock protein 90, ,glucose regulated 0.22 prostaglandin f2-alpha synthase/D-arabinose dehydrogenase 0.22 cystathione gamma lyase, 0.22 alanine racemase 0.22 arginase 0.21 aminopeptidase, ,metallo-peptidase, Clan MG, Family M24 0.21 vacuolar ATP synthase subunit b, 0.21 Transitional endoplasmic reticulum ATPase 0.21 Gim5A protein, ,glycosomal membrane protein 0.21 ATP synthase, 0.21 propionyl-coa carboxylase beta chain, 0.21 3-ketoacyl-CoA thiolase-like protein, 0.20 ADP,ATP carrier protein 1, mitochondrial precursor, ,ADP/ATP translocase 1, 0.20 acetyl-CoA synthetase, 0.20 orotidine-5-phosphate decarboxylase/orotate phosphoribosyltransferase 0.20 glycosomal malate dehydrogenase 0.19 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase 0.19 pyruvate kinase 0.19 ATPase beta subunit 0.18 aconitase 0.18
5.961E7 4.152E7 1.458E8 7.184E7 1.275E7 5.565E7 5.857E7 4.352E7 6.312E7 1.085E7 6.374E7 2.360E7 1.916E7 9.344E7 3.882E7 1.132E7 2.034E7 9.869E7 1.131E7 3.309E7 4.433E7 2.783E7 1.840E7 1.652E7 1.043E7 8.698E7 7.152E6 1.277E8 4.059E7 1.384E7 1.846E7 2.009E7 2.074E7 8.585E7 2.644E7
30.9 48.5 73.7 44.2 67.7 47.8 51.7 50.5 41.7 63.4 40.8 46.0 57.0 39.1 58.2 86.6 31.8 44.5 27.1 36.1 42.5 55.5 86.8 24.9 25.1 56.7 38.1 35.1 78.5 49.5 33.6 30.3 54.2 56.3 97.4
LmjF.35.1010 LmjF.28.2510 LmjF.12.0530 LmjF.31.2970 LmjF.23.0710 LmjF.36.3590 LmjF.29.1570 LmjF.36.1140 LmjF.29.1310 LmjF.36.6650 LmjF.27.0930 LmjF.13.0090 LmjF.36.4170 LmjF.31.1070 LmjF.03.0200 LmjF.33.2340 LmjF.05.0510 LmjF.34.0110 LmjF.35.4430 LmjF.27.1870 LmjF.31.2600 LmjF.29.1960 LmjF.23.0110 LmjF.26.0320 LmjF.31.2250 LmjF.20.1560 LmjF.27.2020 LmjF.05.0960 LmjF.15.1010 LmjF.19.1560 LmjF.20.0110 LmjF.35.1230 LmjF.16.0530 LmjF.06.0860 LmjF.17.0725
casein kinase acyl-CoA dehydrogenase glucose-6-phosphate isomerase acetyl-CoA carboxylase acetyl-CoA synthetase cysteine synthase glutamamyl carboxypeptidase, ,metallo-peptidase, Clan MH, Family M18 short chain 3-hydroxyacyl-CoA dehydrogenase carnitine/choline acetyltransferase 2,3-bisphosphoglycerate-independent phosphoglycerate mutase isovaleryl-coA dehydrogenase carboxypeptidase, ,metallo-peptidase, Clan MA(E), family 32 oxidoreductase, biotin/lipoate protein ligase-like protein delta-1-pyrroline-5-carboxylate dehydrogenase succinyl-coA:3-ketoacid-coenzyme A transferase, mitochondrial precursor ATPase alpha subunit adenylate kinase mitochondrial phosphate transporter trypanothione synthetase calreticulin fumarate hydratase GDP-mannose pyrophosphorylase C-1-tetrahydrofolate synthase, cytoplasmic 3,2-trans-enoyl-CoA isomerase, mitochondrial precursor aminoacylase, ,N-acyl-L-amino acid amidohydrolase D-lactate dehydrogenase-like protein dipeptidyl-peptidase III, ,metallo-peptidase, Clan M-, Family M49 glutamate dehydrogenase inosine-5'-monophosphate dehydrogenase phosphoglycerate kinase B, cytosolic short chain dehydrogenase, dihydroorotate dehydrogenase dihydrofolate reductase-thymidylate synthase guanosine monophosphate reductase
0.18 0.18 0.17 0.17 0.17 0.16 0.16 0.16 0.15 0.15 0.15 0.15 0.14 0.14 0.14 0.13 0.13 0.13 0.13 0.13 0.13 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.11 0.11 0.11 0.11
1.242E7 1.354E7 9.874E6 2.379E8 3.735E7 2.716E7 1.532E7 1.236E7 7.438E6 1.967E7 2.960E7 2.220E7 1.033E7 1.717E7 1.192E7 1.280E7 9.294E7 1.363E7 1.155E7 8.338E6 3.749E7 7.493E6 2.164E7 1.081E7 1.689E7 1.209E7 1.619E7 9.498E6 1.833E7 1.264E7 2.985E7 1.005E7 8.017E6 5.833E6 9.729E6
39.7 68.8 67.1 241.0 77.4 35.4 43.8 33.0 68.6 60.7 44.7 57.0 36.1 28.3 61.9 52.5 62.5 24.4 34.6 74.4 45.1 62.6 41.7 31.7 42.8 42.8 53.7 75.9 115.0 55.5 44.9 28.1 34.6 58.7 58.8
LmjF.31.1220 LmjF.18.0670 LmjF.22.0770 LmjF.30.2900 LmjF.14.0130 LmjF.16.0950 LmjF.12.0670 LmjF.34.0140 LmjF.14.0990 LmjF.21.1340 LmjF.24.0850 LmjF.30.1250
vacuolar-type proton translocating pyrophosphatase 1 citrate synthase, NADH-cytochrome b5 reductase aldehyde dehydrogenase inosine-guanine nucleoside hydrolase sucrose-phosphate synthase-like protein cytochrome c oxidase subunit IV malate dehydrogenase ADP/ATP mitochondrial carrier-like protein ATP synthase, triosephosphate isomerase Pyridoxal kinase
0.11 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10
1.842E7 1.917E7 4.714E6 4.814E6 7.637E6 8.569E6 1.266E7 8.714E7 4.013E6 1.509E7 9.966E6 5.928E6
83.5 50.4 31.8 63.9 39.0 52.4 39.4 33.3 40.5 54.0 27.2 33.2
hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, unknown function hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved
0.26 0.21 0.19 0.17 0.16 0.16 0.16 0.15 0.15 0.15 0.14 0.14 0.14 0.12 0.12 0.12 0.11 0.10 0.10
1.270E7 1.425E7 1.952E7 1.502E7 2.309E7 1.130E6 4.732E6 3.176E7 6.706E6 8.144E6 9.274E6 7.158E6 1.042E7 1.607E7 4.987E6 2.865E6 9.907E6 3.482E6 7.886E6
22.6 59.9 67.2 42.0 55.2 24.2 32.8 71.2 60.6 78.8 36.1 27.4 36.9 21.5 22.3 44.7 90.2 37.7 38.9
Hypothetical protein LmjF.34.2580 LmjF.27.1300 LmjF.09.1010 LmjF.08.1100 LmjF.24.2110 LmjF.32.3210 LmjF.25.2020 LmjF.28.2170 LmjF.22.0300 LmjF.29.1240 LmjF.33.1590 LmjF.23.1410 LmjF.14.0450 LmjF.35.4470 LmjF.14.0190 LmjF.21.0430 LmjF.30.3430 LmjF.15.0040 LmjF.26.2000
LmjF.35.0190 LmjF.35.0900 LmjF.16.0520 LmjF.34.0010 LmjF.32.0950
hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved hypothetical protein, conserved
0.10 0.09 0.09 0.08 0.08
7.256E6 2.611E6 3.429E6 5.173E6 6.004E6
27.2 27.9 36.9 33.6 102.2
proliferative cell nuclear antigen (PCNA) prohibitin, coatomer epsilon subunit PAS-domain containing phosphoglycerate kinase IgE-dependent histamine-releasing factor nucleosome assembly protein nucleosome assembly protein-like protein importin alpha
0.20 0.16 0.14 0.13 0.13 0.13 0.11 0.10
1.735E7 1.153E7 1.013E7 6.219E6 1.403E6 1.845E7 7.796E6 1.050E7
32.4 32.3 35.0 57.5 19.4 39.7 45.4 58.1
Miscellaneous LmjF.15.1450 LmjF.35.0070 LmjF.32.1730 LmjF.30.3380 LmjF.24.1510 LmjF.19.0440 LmjF.31.1750 LmjF.30.1120
