grade 23- meets the requirements of ASTM F136 2002A (AMS 2631B class A1)
were anodized in a mixture of sulphuric/hydrofluoric acid at 20 V for 5 and 60 ...
Journal of Physics: Conference Series
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Bacterial adherence to anodized titanium alloy To cite this article: C Pérez-Jorge Peremarch et al 2010 J. Phys.: Conf. Ser. 252 012011
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Surface Modifications and Functionalization of Materials for Biomedical Applications IOP Publishing Journal of Physics: Conference Series 252 (2010) 012011 doi:10.1088/1742-6596/252/1/012011
Bacterial adherence to anodized titanium alloy C Pérez-Jorge Peremarch1, R Pérez Tanoira1, MA Arenas2, E Matykina2, A Conde2, JJ de Damborenea2, E Gómez Barrena 1, J Esteban1 1 IIS-Fundación Jiménez Díaz Hospital, Madrid, Spain. 2 Centro Nacional de Investigaciones Metalúrgicas CENIM/CSIC. Madrid, Spain E-mail:
[email protected] Abstract. The aim of this study was to evaluate Staphylococcus sp adhesion to modified surfaces of anodized titanium alloy (Ti-6Al-4V). Surface modification involved generation of fluoride-containing titanium oxide nanotube films. Specimens of Ti-6Al-4V alloy 6-4 ELIgrade 23- meets the requirements of ASTM F136 2002A (AMS 2631B class A1) were anodized in a mixture of sulphuric/hydrofluoric acid at 20 V for 5 and 60 min to form a 100 nm-thick porous film of 20 nm pore diameter and 230 nm-thick nanotube films of 100 nm in diameter. The amount of fluorine in the oxide films was of 6% and of 4%, respectively. Collection strains and six clinical strains each of Staphylococcus aureus and Staphylococcus epidermidis were studied. The adherence study was performed using a previously published protocol by Kinnari et al. The experiments were performed in triplicates. As a result, lower adherence was detected for collection strains in modified materials than in unmodified controls. Differences between clinical strains were detected for both species (p