Balance between estrogens and pro inflammatory ...

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Interleukin 6 (complete cds). 1,1. 0,8. Interleukin6 (IFN beta 2). 1,0. 1,2. 9 Interleukin 7. 1,0. 0,9. 10 Interleukin 8. 1,4. 1,1. 11 Interleukin 11. 1,0. 1,3. 12 Interleukin ...
BALANCE BETWEEN ESTROGENS AND PROINFLAMMATORY CYTOKINES REGULATES CHEMOKINE PRODUCTION INVOLVED IN THYMIC GERMINAL CENTER FORMATION Nadine Dragin1, 2, 3,*, Patrice Nancy4, José Villegas2, 3, 5, Régine Roussin6, Rozen Le Panse 2, 3, 5,

and Sonia Berrih-Aknin 2, 3, 5,

1

Inovarion, Paris, France

2

Sorbonne Universités, UPMC Univ Paris 06, Paris, France

3

INSERM U974, Paris, France

4

Department of Pathology, New York University, School of Medicine, New York, USA

5

AIM, institute of myology, Paris, France

6

Hôpital Marie Lannelongue, Le Plessis-Robinson, France

*

Corresponding author

Correspondence and Requests for materials should be addressed to: Dr Nadine Dragin, Inovarion, Centre de Recherche en Myologie, UPMC / INSERM UMRS 974, Groupe Hospitalier Pitié-Salpêtrière, 105 Bd de l’hôpital, 75013 Paris, France, Tel: 00 33 (0)1 40 77 81 27, Fax: 00 33 (0)1 40 77 81 29; [email protected]

Keywords: HLA DR, acetylcholine receptor, CXCL13, Thymic epithelial cells, sex hormones, thymus

Supplementary material Figure S1. Estradiol dose effects on α-AChR mRNA expression in human primary thymic epithelial cells (TECs). Human primary TECs were treated for 24h with 10-10 to 10-7 M of 17- estradiol. α-AChR mRNA level was quantified by real time PCR. Primary cultured TECs were obtained from different donors. The results are expressed as mean values (± SEM). P values were obtained

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-AChR mRNA expression (AU/GAPDH)

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