simple and reliable enough to allow its application in ... cobacterial nucleic acids as template molecules for .... author at the EMail address(s) below. BioTechNet: ...
Address correspondence to Eric S. Ramsson, De- partment of Biomedical Sciences Grand Valley State. University, 208 Henry Hall, 1 Campus Drive, Allen-.
primers are used to amplify mouse. Kdm5c and Kdm5d from genomic DNA, generating amplicons differing by 29 bp. For the rat, we have identified a common.
control experiment, 1% Triton X-100 and. 10 mM CHAPS, without sarkosyl, did not affect GST enzymatic activity. GSH. Sepharose binding was also significantly.
Nov 2, 2011 - ellular staining protocol, which utilizes paraformaldehyde and saponin as the fixative and membrane permeabilizating reagent, respectively, to ...
Aug 1, 2011 - M-R: CGTTCAGGACTCAT ... The mathematical model was established based on the binomial ... Liang, P., J.D. Meade, and A.B. Pardee. 2007.
The green fluorescent protein (GFP) of ... We describe YGFP, a slow bleaching green fluorescent protein (GFP) .... from Discosoma sp. red fluorescent protein.
Wolfe4, Stacey Meeker5, Charlie Hsu5, Lillian Maggio-Price5, and. Craig L. Franklin1,2,3,4. 1University of Missouri (MU) Mutant Mouse Resource and Research ...
Steven R. Head1, H. Kiyomi Komori2, G. Traver Hart2, John Shimashita1, ... The sizing ladder run in the outside lanes is a 100-bp ladder with the 100-bp, 300-bp, and. 600-bp ... samples were of similar high quality, with RIN scores of >9.
only a single exon (chr16: 89,985,667â. 89,986,620). It is a major determinant of the human red hair color phenotype. (red hair and pale skin color) and is also.
Sep 12, 2012 - QR Code or visit the App Store and search for. âBioTechniquesâ to download. Monthly. Issues Now. Available for iPad and. iPhone in the iTunes.
response effect of melanin on RT-PCR inhibition and the effect of BSA in overcoming melanin inhibition. The addition of 2 µg of synthetic melanin. (Catalog No.
Oct 22, 2012 - BLAST suite for Unix systems, but it is ... Keywords: BLAST+; stand-alone; graphical user interface; GUI; local databases .... Email: jhramirez@.
tion, the Schweppe Foundation, and the. Brinson Foundation (T.-C.H.). We wish to thank Bert Vogelstein of Johns Hopkins. Oncology Center for the provision of.
Feb 20, 2012 - Imaging www.BioTechniques.com/rd. 1. BioTechniques Rapid ... Antibodies are important drugs for treating cancer and there is strong rationale ...
Address correspon- dence to Dr. ... Reaves Hall, Virginia Tech, Blacksburg, VA. 24061 ..... Florida. We thank Ginger Clark for critical- ly reading the manuscript.
region of a binary expression vector (Supple- ... promoter, thereby creating an internal .... (A) Illustration depicting the T-DNA of the pBiFCt-2in1-NC vector.
Lee, R.T., F. Berditchevski, G.C. Cheng and ..... chim. Biophys. Acta 1489:263-280. 2.Petry, F., K.B.M. Ried and M. Loos. ... guez, T. Boon and J.-C. Cerottini.
Imaging. Molecular. Biology. Cell Biology www.BioTechniques.com/rd. 1 ... and Biophysics, Institute of Molecular Cell and Systems Biology, University of.
Address correspondence to Dr. Gary E. Truett, Developmental Genetics Laborato- ry, Pennington Biomedical Research Center,. 6400 Perkins Road, Baton ...
For the RNase-treated specimens, slides containing fixed squashed sali- vary gland cells that had incorporated. Bromo-Uridine were washed briefly with 2´ SSC.
the insert contains a site for the same enzyme. This problem can ... Enzyme-free cloning (EFC) (1â3) is a good ... Table S1) to create the insert fragment. These.
MS) due to their high sensitivity and .... quence represented by each peptide observed following MALDI-MS of 0.5 µL .... conditions that increase the mis-incor-.
of each PCR reagent. The objective ... PCR reagents were obtained from Immunogen International (Sunderland, .... action in an anemic mode: how to avoid cold.
Figure S1. Mean fluorescence values (normalized) of real-time potato spindle tuber viroid (PSTVd) loop-mediated isothermal amplification (LAMP) assays with ...
Benchmarks Supplementary Material For:
Shining a light on LAMP assays—A comparison of LAMP visualization methods including the novel use of berberine Jens Fischbach, Nina Carolin Xander, Marcus Frohme, and Jörn Felix Glökler Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Wildau, Germany BioTechniques 58:189-194 (April 2015) doi 10.2144/000114275 Keywords: berberine; calcein; hydroxynaphthol blue; LAMP; loop-mediated isothermal amplification; potato spindle tuber viroid; SYBR Green I; EvaGreen
Figure S1. Mean fluorescence values (normalized) of real-time potato spindle tuber viroid (PSTVd) loop-mediated isothermal amplification (LAMP) assays with serially diluted DNA. The sensitivity of all assays was determined after incubation at 65°C for 60 min with decreasing amounts of DNA. SYBR Green I (A), EvaGreen (B), and berberine (C). All assays were monitored in an ESEquant Tube Scanner with the FAM filter (Exc.: 470 nm/ Em.: 520 nm). Template copy numbers are 1.5 × 1010 (black), 1.5 × 10 8 (blue), 1.5 × 10 6 (red), 1.5 × 10 4 (green), 1.5 × 10 2 (orange), 1.5 × 10° (magenta), and no template (gray). Detection sensitivity for each dye in the real-time LAMP assay is shown as threshold time of the fluorescence signal above 0.2 RFU (y-axis) against the log copy number of the initial template DNA (x-axis). Experiments were done in triplicate. Regression equation and coefficient of determination are shown as an insert. The data point for log 4 of SYBR Green I was not included in the regression analysis.
Vol. 58 | No. 4 | 2015
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BENCHMARKS
Figure S2: Effect of berberine concentration on loop-mediated isothermal amplification (LAMP) reaction kinetics. The real-time LAMP assay (A) was carried out with 180 µM (1), 160 µM (2), 140 µM (3), 120 µM (4), 100 µM (5), 80 µM (6), 60 µM (7), and 40 µM (8) berberine by monitoring the reaction with the ESEQuant Tube Scanner (FAM filter, Exc.: 470 nm/Em.: 520 nm) for 60 min at 65°C. A template amount of 1.5 × 1010 copies was used as the positive control. All LAMP assays start amplification after 10 min, with absolute fluorescence intensity correlating with dye concentration. Insert shows a picture of the reaction tubes in UV light (312 nm). Experiments were performed in triplicate. The standard deviation and the average value of the linear slope for each berberine concentration show the effect on the reaction kinetics (B).
