bicuspid aortic valve (BAV). TAV AA streamlined flow streamlined orifice jet. BAV AA skewed BAV orifice jet helical flow. (Mahadevia et al. Circ 2014). To isolate ...
Bicuspid Aortic Valve Hemodynamics Induce Acute Asymmetric Remodeling of Porcine Ascending Aortas: An Ex Vivo Study Samantha K. Atkins, Philippe Sucosky
University of Notre Dame, Department of Aerospace and Mechanical Engineering, Notre Dame IN
Tissue Preparation longitudinal cut
◊ Bicuspid aortic valve (BAV) is the most common congenital cardiac anomaly present in 1-2% of the population Type-I left-right (LR) bicuspid aortic valve (BAV) rightraphe coronary fused leaflet leaflet
Shear Stress Bioreactor
sample extracted from wall concavity
sample extracted from wall convexity
Tricuspid aortic valve (TAV) leftcoronary leaflet
healthy porcine aorta
tissue samples isolation
Tissue Conditioning to WSS
tissue samples sutured on plate
◊ Samples from AA convexity exposed to TAV AA or BAV AA convexity WSS ◊ Samples from AA concavity exposed to TAV AA or BAV AA concavity WSS TAV AA WSS
streamlined flow
6.0
Convexity Concavity
WSS (Pa)
◊ BAV anatomy is a major risk factor for secondary aortopathies ◊ Abnormal hemodynamics generated by type-I LR BAV seems to correlate with asymmetric presentation of ascending aortic dilation on wall convexity
6.0
WSS (Pa)
noncoronary leaflet
noncoronary leaflet
BAV AA WSS
3.0 0.0
◊ Standard culture medium ◊ 48-hour culture duration ◊ Immunoblotting, immunostaining and zymography were performed to quantify remodeling response
0.0
-3.0
-3.0
-6.0 0
0.43 time (s)
0
0.86
0.43 time (s)
0.86
skewed BAV orifice jet BAV AA (Mahadevia et al. Circ 2014)
TAV AA BAV AA Fresh WSS WSS
pro MMP-9 active MMP-9 pro MMP-2 active MMP-2
Tissue from convexity subjected to TAV AA and BAV AA convexity WSS
Tissue from concavity subjected to TAV AA and BAV AA concavity WSS
% change vs. fresh
BAV AA WSS upregulates MMP-2 and MMP-9 activity in the AA convexity but not in the concavity TAV AA BAV AA Fresh WSS WSS
