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World J Gastroenterol 2004;10(17):2547-2549 World Journal of Gastroenterology Copyright © 2004 by The WJG Press ISSN 1007-9327
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Changes of mRNA expression of enkephalin and prodynorphin in hippocampus of rats with chronic immobilization stress Jia-Xu Chen, Wei Li, Xin Zhao, Jian-Xin Yang, Hong-Yan Xu, Zhu-Feng Wang, Guang-Xin Yue Jia-Xu Chen, Wei Li, Xin Zhao, Jian-Xin Yang, Hong-Yan Xu, ZhuFeng Wang, Guang-Xin Yue, School of Basic Medical Science, Beijing University of Traditional Chinese Medicine, Beijing 100029, China Supported by the National Natural Science Foundation of China, No. 30000216, Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutes of Ministry of Education, China, Foundation for the Authors of National Excellent Doctoral Dissertations of China, No. 200059, and Fok Ying Tong Education Foundation, No. 81037 Correspondence to: Dr. Jia-Xu Chen, Deputy of TCM Diagnosis Department, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
[email protected] Telephone: +86-10-64287074 Received: 2003-12-23 Accepted: 2004-01-16
Abstract AIM: To observe the changes of enkephalin mRNA and prodynorphin mRNA in hippocampus of rats induced by chronic immobilization stress. METHODS: Thirty rats were randomly divided into three groups of 10 each: the normal control group (group A), the group induced by chronic immobilization stress for 7 d (group B) and the group induced by chronic immobilization stress for 21 d (group C). The changes of the enkephalin mRNA and prodynorphin mRNA in the rat hippocampus were detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Expression levels of enkephalin mRNA and prodynorphin mRNA in rat hippocampus were significantly increased under chronic immobilization stress, and the expression of prodynorphin mRNA in the rat hippocampus in group C was remarkably higher than that in group B (0.624±0.026; n = 5; P
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