Slalom chromatography (for DNA). -. Diff. in length and flexibility. Interactive modes of liquid chromatography. Ion-exchange chromatography. IEC. Electrostatic ...
P3
Chromatography THE 2ND SUMMER SCHOOL IN INTERDISCIPLINARY SCIENCES 21-24 September 2009
Presenter: H. Zamani Supervised by Dr R. Yousefi (Assistant Professor of Biochemistry). Co-supervisor: Dr. H. R. Karbalaei (Assistant Professor of Biochemistry). Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran
What is chromatography
Chromatographic separation
Plumber’s view of chromatography
Invented by a Russian botanist named Mikhail Tswett in 1903. He separated plant pigments using glass columns packed with calcium carbonate.
Illustration of chromatography
Qualitative and quantitative analysis
Chromatogram
Types of chromatography
Resolution of separation
( on the basis of interaction of the analyte with stationary phase)
Basic chromatographic terminology
Partition coefficient KD
How good is this column for separation Chromatographic Modes of Protein Purification Chromatographic Mode
Acrony m
Separation Principle
Non-interactive modes of liquid chromatography Size-exclusion chromatography
SEC
Differences in molecular size
Slalom chromatography (for DNA)
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Diff. in length and flexibility
Interactive modes of liquid chromatography Ion-exchange chromatography
