COMBINED EFFECTS OF TESTOSTERONE ...

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dependent sex glands weight were measured. Fructose and prostaCc acid phosphatase (PAP) were also measured in prostate and seminal vesicle. Treatment.
COMBINED EFFECTS OF TESTOSTERONE PROPIONATE AND Leptadenia hastata PERS. (DECNE) AQUEOUS EXTRACTS ON IMMATURE CASTRATED MALE RATS Bayala Balé, UFR/SVT, University of Ouagadougou, BURKINA FASO

Karlsruhe, 28-29 November 2012, Germany, Alexander Von Humboldt Foundation Meeting INTRODUCTION   Leptadenia  hastata  (Pers.)  Decne.    is  a  perennial  liana  species  of  Asclepediacea  family  and  it  is  widely  used  in  African   tradi9onal   medicine.   L.   hastata     have   been   reported   to   be   rich   in   steroidal   glycosides,   cardenolides,   flavonoids,   triterpenes   and   polyoxypregnane   deriva9ves.   In   the   north   of   Burkina   Faso,   where   livestock   is   very   important,   the   breeders  claimed  the  loss  of  fer9lity  of  their  animals  aEer  the  consump9on  of  L.  hastata  leaves.  The  purpose  of  the   present  study  was:  first,  to  determine  the  compe99on  between  L.  hastata  extracts  and  testosterone  propionate  (TP)   as  androgen  reference  in  vivo;  and  second,  to  evaluate  the  an9-­‐androgenic  ac9vity  of  L.  hastata  extracts.       MATERIAL  AND  METHODS   150  g  of  the  leaves  of  L.  hastata  were  collected,  washed,    dried,  macerated  and  lyophilized.  Three  weeks  old  Wistar   male   rats   were   castrated   and   randomized   into   2   treatment   groups.   Each   group   was   divided   into   4   subgroups   of   6   animals.   The   four   subgroups   of   the   first   group   received   dis9lled   water   (as   control),   100,   200   and   400   mg/kg   of   L.   hastata  aqueous  extracts  respec9vely.  For  the  second  group,  each  subgroup  received  in  combina9on  0.4  mg/kg  of  TP   and  the  same  amounts    of  L.  hastata  aqueous  extracts.  TP  was  administered  subcutaneously  and  L.  hastata  extracts  by   oral   gavage   during   10   consecu9ve   days.   24   h   aEer   the   last   administra9on,   the   rats   were   killed   and   androgen-­‐ Bayala et al. 2927 dependent  sex  glands  weight  were  measured.  Fructose  and  prosta9c  acid  phosphatase  (PAP)  were  also  measured  in   prostate  and  seminal  vesicle.  

 

S. vesicle(L.h) S. vesicle(TP+L.h)

100

   

Seminale vesicles (mg)

80

*a

60

aa** *

40

** **

RESULTS!

L.   hastata   aqueous   extracts   were   found,   compared   to   control   groups   to   reduce   significantly   (p