316 Characterization of Apple Allergy in A Mediterranean Population. Marıa José ... Spain, 5Biotechnology Department, Center for Plant Biotechnology and.
AB88 Abstracts
316
SUNDAY
Characterization of Apple Allergy in A Mediterranean Population Marıa Jose Torres, MD, PhD1, Francisca Gomez, MD, PhD1, Ana Aranda, PhD2, Cristobalina Mayorga, PhD3,4, Ignacio Garcia, MD, PhD1, Araceli Diaz-Perales, PhD5, Natalia Blanca-Lopez, MD, PhD6, Elena Seoane, MD, PhD6, Arantxa Palacin, PhD5, Maria Garrido, PhD5, Stefan Vieths, PhD7, Miguel Blanca, MD, PhD1; 1Allergy Service, Carlos Haya Hospital, Malaga, Spain, 2Research Laboratory, Carlos Haya Hospital-FIMABIS, Malaga, Spain, 3Research Laboratory, Carlos Haya Hospital, Malaga, Spain, 4Pediatric Service, Carlos Haya Hospital, Malaga, Spain, 5Biotechnology Department, Center for Plant Biotechnology and Genomics, Madrid, Spain, 6Allergy Service, Infanta Leonor Hospital, Madrid, Spain, 7Paul-Ehrlich-Institut, Langen, Germany. RATIONALE: Studies from different populations have shown two different clinical entities in apple allergy, oral allergy syndrome (OAS) and systemic symptoms, reflecting different patterns of allergen sensitization. We analyzed the pattern of sensitization to a wide panel of inhalant, fruits, nuts allergens and different components (Mald1, Mald2, rMald3, rMald4, rPrup3, rBetv1 and Phold2) in a population of Mediterranean patients allergic to apple. METHODS: Patients (N581) with a clinical history of allergy to apple confirmed by positive skin prick test (SPT) and double blind placebo controlled food challenge (DBPCFC) were included. SPT and ELISA were done with a panel of inhalant, fruit, nut allergens and to the different components detailed above. ELISA inhibition studies were done for analyzing pattern of sensitization. RESULTS: Thirty-five cases (43.2%) had OAS and 46 (56.8%) systemic symptoms. SPT showed higher positive results with peach, cherry and hazelnut in those with systemic symptoms. ELISA showed significantly higher percentage of positivity in patients (OAS and systemic) when compared to controls for rMald3, rMald4, rPrup3, Olive, Birch and Tymothy grass; between those with systemic symptoms and controls for hazelnut; and between OAS and controls for Betv1. Three different patterns of recognition were detected: positive to LTP (rMald3 and/or rPrup3), to profilin (rMald4 and/or Phold2) or to both, being the latter most frequently detected in those with systemic symptoms. CONCLUSIONS: This study indicates that when analyzing a big population with apple allergy different patterns of sensitization may appear with those with systemic symptoms mainly induced by LTP.
317
Food Specific IgE Levels in Children Should Be Interpreted in Context of Total IgE Tara J. Federly, MD1,2, Bridgette L. Jones, MD, FAAAAI1, Hongying Dai, PhD1, Chitra Dinakar, MD, FAAAAI1,2; 1Children’s Mercy Hospital & Clinics, Kansas City, MO, 2University of Missouri-Kansas City. RATIONALE: Food specific IgE (fsIgE) cut-points are used in the evaluation of food allergies. Concomitant measurement of total IgE (tIgE) is traditionally not performed. We have anecdotally observed elevations in fsIgE levels mirroring tIgE increases, confounding accurate interpretation. We aimed to determine if a) changes in tIgE correlated with fsIgE and b) predictions of fsIgE could be formulated based on tIgE. METHODS: Subjects were children evaluation. After IRB approval, log-transformed (ln) tIgE and fsIgE levels were retrospectively analyzed using univariate and multivariate regression models to assess associations and to predict fsIgE using tIgE and other covariates. RESULTS: Soy IgE levels were strongly correlated (Pearson correlation coefficient rho50.85, p
