Lasers Med Sci DOI 10.1007/s10103-013-1400-8
ORIGINAL ARTICLE
Effect of antimicrobial photodynamic therapy on periodontally infected tooth sockets in rats Leticia Helena Theodoro & Juliana Rico Pires & Leandro Araújo Fernandes & Erivan Clementino Gualberto Júnior & Mariéllen Longo & Juliano Milanezi de Almeida & Valdir Gouveia Garcia
Received: 23 January 2013 / Accepted: 15 July 2013 # Springer-Verlag London 2013
Abstract The aim of the present study was to evaluate the antimicrobial effect of antimicrobial photodynamic therapy (aPDT) in alveolar treatment of areas with induced periodontitis. Thirty male Wistar rats were subjected to ligature-induced periodontal disease (PD) in the first left inferior molars, while the right side molars did not receive ligatures. After 7 days of PD evolution, ligatures were removed from the left side, and the first left and right mandibular molars were extracted. Afterwards, animals were divided into groups according to the following treatments: control (C)—no treatment; mechanical debridement (MD)—mechanical debridement and irrigation with saline solution; and aPDT—mechanical debridement, irrigation with toluidine blue O (TBO), and 1 min of laser irradiation (GaAlAs, 660 nm, 30 mW, 32 J/cm2, 60 s). Ligatures were removed and samples of the alveolar content after extraction and after each treatment were collected for microbial processing by real-time polymerase chain reaction with specific primers for Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Treponema denticola. Data L. H. Theodoro : E. C. Gualberto Júnior : M. Longo : J. M. de Almeida : V. G. Garcia Group of Study and Research on Lasers in Dentistry (GEPLO), Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University (Universidade Estadual Paulista—UNESP), Araçatuba, SP, Brazil J. R. Pires : V. G. Garcia Dental School of Barretos, University Center of the Educational Foundation of Barretos (UNIFEB), Barretos, SP, Brazil L. A. Fernandes Department of Clinical and Surgery, Federal University of Alfenas (Universidade Federal de Alfenas—UNIFAL), Alfenas, MG, Brazil L. H. Theodoro (*) Faculdade de Odontologia de Araçatuba, UNESP, Rua José Bonifácio 1193, Centro, CEP: 16050-300 Araçatuba, SP, Brazil e-mail:
[email protected]
were submitted to statistical analysis by multiple comparison tests (McNemar test; p
