Effect of Buffer Composition, Sephadex Grade and Column Size on ...
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Effect of Buffer Composition, Sephadex Grade and Column Size on Filtration Based Quality Improvement of Semen from Murrah Buffalo Bull V. P. Maurya*, R. K. Tuli1 and R. L. Goyal1 Division of Animal Physiology, Central Sheep and Wool Research Institute Avikanagar, Rajasthan 304501, India ABSTRACT : Sixty semen ejaculates collected at weekly interval from four Murrah Buffalo bulls over a period of seven months (Nov.1999 to May 2000) were used in the present study. Three buffer medium (sodium citrate, TES and Tris) were used for soaking of sephadex. Three grades of sephadex (G-15,G-100, and G-200) were used for preparation of columns. Columns of three different height (one, two and three cm) were used for separation of semen. Twenty semen ejaculates were used in each project. In the first experiment each semen ejaculates was divided into four parts. One part was kept as control and other three parts were passed thought one cm column of sephadex G-15 prepared in three different buffers. There was significant (p
