In: Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery: Electrically Mediated Delivery of Molecules to Cells. Jaroszeski M, Heller R and ...
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 8: 489-494, 2001
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Elevated gastrin secretion by in vivo gene electroporation in skeletal muscle AKIHIRO YASUI1, KOJI ODA2, HIROTOSHI USUNOMIYA3, KENICHI KAKUDO3, TAKAYUKI SUZUKI4, TOYONOBU YOSHIDA4, HYI-MAN PARK4, KAZUTERU FUKAZAWA4 and TATSUO MURAMATSU4 1Department
of Surgery, Chubu National Hospital, Ohbu 474-8511; 2Department of Surgery, Nagoya University Graduate School of Medicine, Nagoya 466-8550; 3Second Department of Pathology, Wakayama Medical College, Wakayama 640-8155; 4Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan Received June 26, 2001; Accepted July 25, 2001
Abstract. Whether or not in vivo gene transfer of gastrin gene into skeletal muscle by electroporation could modify gastrin secretion was examined. The expression plasmid vector, either pMEPrGaspA encoding the rat gastrin gene or pEGFP-N1 encoding the GFP reporter gene was injected into M. rectus abdominis of rats or M. biceps formis of mice. Subsequently, square electric pulses of direct current were applied six times at 25 V with a loading period of 100 msec per pulse. Clear foreign gene expression in the skeletal muscle was demonstrated by both GFP fluorescence and immunostaining of rat gastrin. Time course changes in plasma gastrin levels after transfection revealed that in rats, gastrin gene transfer significantly increased the plasma gastrin level for 4 weeks post-transfection (P
