Evolution of the shut-off steps of vertebrate phototransduction Trevor D. Lamb1, Hardip R. Patel2, Aaron Chuah3, and David M. Hunt4,5
1
Eccles Institute of Neuroscience, 2National Centre for Indigenous Genomics, and 3Genome Discovery Unit, John Curtin School of Medical Research, The Australian National University, ACT 2600, Australia, and 4The Lions Eye Institute and 5School of Biological Sciences, The University of Western Australia, WA 6009, Australia.
Contents Page Supplementary Table S1. Summary of transcripts examined.
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Supplementary Table S2. Multiple sequence alignment for GRKs.
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Supplementary Table S3. Multiple sequence alignment for arrestins.
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Supplementary Table S4. Multiple sequence alignment for RGS9/11s.
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Supplementary Table S5. Multiple sequence alignment for Gβ5s.
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Supplementary Table S6. Multiple sequence alignment for R9APs.
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2 Supplementary Table S1. Summary of transcripts examined #
Notes, and explanation of columns Column 3, ‘Label on Figures’: Sequences are indicated ‘-Partial’ if they included fewer than 95% of the expected residues. Entries in square brackets do not appear in phylogenies because the protein sequence was identical to another sequence included in the tree; for example, all entries for Eastern ray were omitted in favour of the identical protein sequences for Western ray. Column 6, ‘Join’: An entry ‘J’ indicates that the sequence was manually joined from more than one contig. These joined sequences overlapped, except where indicated ‘NJ’ for a noncontiguous join. Column 7: An entry ‘M’ indicates that the sequence started with a Met. Column 8. An entry ‘*’ indicates that sequence ended with a stop codon. Column 9: RPKM = Reads per kilobase per million mapped reads. Column 10: RPKM-CDS = Same, but calculated across only the coding region of the transcript.