by RT-qPCR (B) WT and Atf4 KO naïve CD4+ T cells were stimulated under the Th1 condition with or without β-ME for 24h. mRNA levels of Glyt1 were analyzed ...
Cell Reports, Volume 23
Supplemental Information
ATF4 Regulates CD4+ T Cell Immune Responses through Metabolic Reprogramming Xi Yang, Rui Xia, Cuihua Yue, Wensi Zhai, Wenwen Du, Qianting Yang, Huiling Cao, Xiaojuan Chen, Danielle Obando, Yibei Zhu, Xinchun Chen, Jane-Jane Chen, Jon Piganelli, Peter Wipf, Yu Jiang, Guozhi Xiao, Changping Wu, Jingting Jiang, and Binfeng Lu
Supplementary figures
A
Asns *** ***
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Figure S1. Induction of ATF4 in the thiol-oxidizing environment. Related to figure 1. (A) Naïve CD4+ T cells were stimulated under the Th1 condition with β-ME or NAC or media alone for 24h. mRNA levels of the ATF4 downstream genes Asns and Glyt1 were analyzed by RT-qPCR (B) WT and Atf4 KO naïve CD4+ T cells were stimulated under the Th1 condition with or without β-ME for 24h. mRNA levels of Glyt1 were analyzed by RT-qPCR. (C) WT CD4+ T cells were isolated from spleens and lymph nodes and stimulated for 0h, 24h,96h and re-stimulated by plate-bound anti-CD3 for 4h (96+4). Nuclear extracts were made and analyzed by western blot. (D) Primary CD4+ T cells were stimulated under Th0 and Th1 conditions for 24h. Levels of ATF4 mRNA were analyzed by RT-qPCR. *** P
