Hamster Kidney Tumor Cells Estrogen-sensitive ...

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Estrogen-sensitive Proliferation Pattern of Cloned Syrian Hamster Kidney Tumor. Cells1 ... (GIBCO, Grand Island, NY) supplemented with 5% heat-inactivated.
Estrogen-sensitive Proliferation Pattern of Cloned Syrian Hamster Kidney Tumor Cells Ana M. Soto, James C. Bass and Carlos Sonnenschein Cancer Res 1988;48:3676-3680.

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[CANCER RESEARCH 48, 3676-3680, July 1, 1988]

Estrogen-sensitive Proliferation Pattern of Cloned Syrian Hamster Kidney Tumor Cells1 Ana M. Soto,2 James C. Bass, and Carlos Sonnenschein Tufts University School of Medicine, Department of Anatomy and Cellular Biology, Boston, Massachusetts 02111

ABSTRACT Estrogen-sensitive hamster kidney tumor cells H301 and their clonal derivatives were inhibited from proliferating in culture by charcoaldextran (CD) stripped serum in a dose-dependent manner. Homologous serum was more potent an inhibitor than heterologous (human, bovine, equine) sera. Natural and synthetic estrogens failed to increase the proliferation rate of cells maintained in 2% CD Syrian hamster serum (SHS). At CDSHS concentrations above 2%, cell proliferation was significantly inhibited and estrogens completely reversed this inhibitory effect. Nonestrogenic steroids failed to overcome the serum inhibition. Two synthetic estrogens, moxestrol and 11/i-chliironici li>lostradiili, were 10-fold more potent than estradiol in increasing cell proliferation yields; they were however, less potent than estradiol in inhibiting [3H]estradiol binding to intracellular estrophilins.