HPLC Measurement, Bbod Distribution, and ... - CiteSeerX
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HPLC Measurement, Bbod Distribution, and ... - CiteSeerX
HPLC Measurement, Bbod Distribution, and Pharmacokinetics of Oral Clotrimazole, Potentially Useful Antisickling Agent Nader Rifai,”2” Masiyuki and Carlo Brugnara”
Clotrimazole (CLI) has recently been shown to be a potent and specific inhibitor of the Ca2-activated K channel and to thereby prevent K loss and cellular dehydration of sickled erythrocytes. This evidence suggests that oral CLI may be a useful new therapy for sickle cell disease. Here, we describe the development of an HPLC assay to measure CLT, a method we used to study the pharmacokinetics and transport of CLT in normal volunteers. The assay’s linear range extended to 10 moVL; the detection limit was 0.1 moVL, analytical recovery 97.7%, and run-to-run imprecision (CV)
