Oct 22, 2008 - ... C. Molony,1 Eoin Sheehan,1 Damian J. McCormack,1 James P. O'Gara2,3 ..... research, and H. Humphreys and T. Dillane, Department of.
Hydrogen Peroxide Induced Repression of icaADBC Transcription and Biofilm Development in Staphylococcus epidermidis Aaron A. Glynn,1 Sinead T. O’Donnell,2,3 Diarmuid C. Molony,1 Eoin Sheehan,1 Damian J. McCormack,1 James P. O’Gara2,3 1 Cappagh National Orthopaedic Hospital, Ratoath Road, Finglas, Dublin 11, Ireland, 2Department of Clinical Microbiology, Education and Research Centre, Beaumont Hospital, Royal College of Surgeons in Ireland, Dublin 9, Ireland, 3School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland
Received 16 May 2008; accepted 22 July 2008 Published online 22 October 2008 in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jor.20758
ABSTRACT: Expression of the icaADBC-encoded polysaccharide intercellular adhesion by Staphylococcus epidermidis promotes biofilm formation and represents an important virulence factor in biomaterial-related infections following orthopedic surgery. Biofilm development by the pathogen can be viewed as a protective reaction to environmental stressors including osmotic stress, thermal stress, and antimicrobial chemotherapy. Oxidative stress, arising from the release of toxic oxygen radicals by polymorphonuclear cells, is encountered by bacteria entering the body perioperatively. Evasion of this and other cell-mediated immune responses by pathogenic bacteria plays an important role in the development of chronic biomaterial-related infection. Here we investigated the impact of sublethal oxidative stress induced by H2O2 (
