Implementation and application of a multiplex assay to detect malaria ...
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Implementation and application of a multiplex assay to detect malaria ...
Sep 4, 2015 - Background. Epidemiological surveillance is a key activity in malaria control and elimination in low-transmission and pre-elimination settings.
Kerkhof et al. Malar J (2015) 14:338 DOI 10.1186/s12936-015-0868-z
Open Access
RESEARCH
Implementation and application of a multiplex assay to detect malaria‑specific antibodies: a promising tool for assessing malaria transmission in Southeast Asian pre‑elimination areas Karen Kerkhof1,2* , Lydie Canier3, Saorin Kim3, Somony Heng4, Tho Sochantha4, Siv Sovannaroth4, Inès Vigan‑Womas5, Marc Coosemans1,2, Vincent Sluydts1,6, Didier Ménard3 and Lies Durnez1*
Abstract Background: Epidemiological surveillance is a key activity in malaria control and elimination in low-transmission and pre-elimination settings. Hence, sensitive tools for estimating malaria force of infection are crucial. Serological mark‑ ers might provide additional information in estimating force of infection in low-endemic areas along with classical parasite detection methods. Serological markers can be used to estimate recent, past or present malaria exposure, depending on the used markers and their half-life. Methods: An assay based on 14 Plasmodium-specific peptides, one peptide specific for Anopheles gambiae saliva protein and five Plasmodium-specific recombinant proteins was developed for the MAGPIX system, assessed for its performance, and applied on blood spots from 2000 individuals collected in the Ratanakiri Province, Cambodia. Results: A significant correlation for the use of 1000 and 2000 beads/antigen/well as well as for the monoplex versus multiplex assay was observed for all antigens (p
