3,4 Medical School of the Pontifical Catholic University of Paraná, Curitiba/PR, Brazil;. 5 Positivo University, Curitiba/PR, Brazil;. 6 Curitiba Ophthalmic Centre, ...
ORIGINAL ARTICLE
10.5935/0034-7280.20140046
210
Increased intercellular adhesion molecule-1 immunoreactivity in the sclera-choroid complex in hypercholesterolemia experimental model Aumento da imunorreatividade da molécula de adesão intercelular-1 na esclera e coroide em modelo experimental de hipercolesterolemia Rogil José de Almeida Torres1, Lucia de Noronha2, Regiane do Rocio de Almeida Torres3, Seigo Nagashima4, Caroline Luzia de Almeida Torres5, Andréa Luchini6, Robson Antônio de Almeida Torres5, Leonardo Brandão Précoma7, Dalton Bertolim Précoma8
ABSTRACT Objective: The aim of this study is to investigate the expression of the intercellular adhesion molecule 1 (ICAM-1) in the sclera and choroid of hypercholesterolemic rabbits. Methods: New Zealand rabbits were divided into two groups: the normal diet group (NG), with 8 rabbits (8 eyes), was fed a standard rabbit diet for 4 weeks; the hypercholesterolemic group (HG), with 13 rabbits (13 eyes), was fed a 1% cholesterol- enriched diet for 8 weeks. Total serum cholesterol, triglyceride, HDL cholesterol and fasting blood glucose exams were performed at the start of the experiment and at the euthanasia time. HG and NG animals were euthanized after 8th week and 4th week, respectively. Their eyes were stained with hematoxylin-eosin and underwent histological, histomorphometric and immunohistochemical analyses with ICAM-1 antibody. Results: The diet has induced a significant increase in total cholesterol and triglyceride levels in HG when compared with NG (p
