Journal of Visualized Experiments
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Materials List for:
Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection 1
1
2,3
1
2
Ombretta Guardiola , Gennaro Andolfi , Mario Tirone , Francescopaolo Iavarone , Silvia Brunelli , Gabriella Minchiotti
1
1
Stem Cell Fate Laboratory, Institute of Genetics and Biophysics "A. Buzzati Traverso", CNR
2
School of Medicine and Surgery, University of Milano-Bicocca
3
Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute
Correspondence to: Ombretta Guardiola at
[email protected] URL: http://www.jove.com/video/54515 DOI: doi:10.3791/54515
Materials Name
Company
Catalog Number
Cardiotoxin from Naja mossambica SIGMA ALDRICH mossambica
C9759
Syringe For Insulin BD Micro-Fine+ BD Needle 30 G x 8 mm - Da 0.3 mL
324826
Tragacanth Gum
MP BIOMEDICALS,LLC
104792
2-Methylbutane (Isopentane)
SIGMA ALDRICH
78-78-4.
OCT Killik Solution For Inclusion Cryostat
Bio-optica
05-9801
Feather Microtome Blade S35
Bio-optica
01-S35
Glass Slide Superfrost Plus
Menzel-Gläser
09-OPLUS
Dumon #5 Mirror Finish Forceps
2BIOLOGICAL INSTRUMENTS
11251-23
Scissors Straight Sharp/Sharp
2BIOLOGICAL INSTRUMENTS
15024-10
Scissors Noyes Straight
2BIOLOGICAL INSTRUMENTS
15012-12
Fine Iris Scissors Straight Sharp/ Sharp 10.5 cm
2BIOLOGICAL INSTRUMENTS
14094-11
Eukitt
Bio-optica
09-00100
Slide Coverslip
BIOSIGMA
VBS651
Xylene
SIGMA ALDRICH
214736
Ethanol 100%
sigma-Aldrich
02860-2.5L
Hematoxyline
J.T. BAKER
3873
Eosin
SIGMA ALDRICH
HT110116
Cryostat
LEICA
CM3050 S
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