Instability Drives Replication Stress, Abnormal Development, and ...
Recommend Documents
May 10, 2018 - Yellow arrows indicate scored chromosomal aberrations such as breaks or ..... B Alcian blue/Alizarin red staining. B MicroCT scan. B Rotarod ...
May 10, 2018 - Polε Instability Drives Replication Stress,. Abnormal Development, and Tumorigenesis. Roberto Bellelli,1,5 Valerie Borel,1,5 Clare Logan,2 ...
Aug 1, 2011 - Technology & Medicine ... Peking University Health Science Center, ...... Huen MS & Chen J (2008)
Aug 1, 2011 - ATM and ATR are at the top of the DNA damage pathways. ... cell cycle arrest, DNA repair, and apoptosis (L
Aug 19, 2011 - hydroxyurea) or DNA-damaging agents (methyl methanesulfonate or ultraviolet light). Aneuploid strains derived by triploid meiosis also ...
2. Even in the linear formulation of the atmospheric instability problem the transition from stable to ...... 8. Square root of perturbation kinetic energy contributed by.
Mar 6, 2015 - PS] 6 Mar 2015. Observation of Optical Solitons and Abnormal Modulation Instability in Liquid. Crystals with Negative Dielectric Anisotropy.
Apr 17, 2009 - Eleven cases were chosen who had CD8+ T cell CD38+HLA-DR+ expression .10% and plateau absolute .... RoboGo (MWG Inc.) pipetting station and analyzed on a MJR. Opticon II ..... Dis Mar 5: [Epub ahead of print]. 8.
Aug 16, 2012 - ... La Jolla, California, USAa; Department of Medical Biochemistry and Biophysics, .... Freon-trioctylamine mixture (10 ml of 99% pure Freon [1,1,2-trichloro- ..... this proposition, we monitored Rad11-GFP foci in dcd1 cells.
Apr 24, 2012 - Center, San Antonio, Texas, USAc; Cancer Therapy and Research Center, University of Texas Health Science Center, San Antonio, Texas, ...
6 days ago - Editor: Julian E. Sale, MRC Laboratory of Molecular ..... this short DRS resulted in a substantial increase of segregation defects in these cells.
Aug 25, 2008 - branching pattern, while in the hypoxic ones there was often only an irregular ..... Cells were counted in Adobe Photoshop 7.0 (Adobe Sys- tems Inc. ..... AâE: illustration of typical activation patterns in frontal view at. ED3, 4, 6
Feb 20, 2008 - knock out mice lacking CYR61 show that it is required for placental development and vascular integrity [25]. Muta- tions in a third CCN gene, ...
Mar 7, 2012 - IAA-Ala, indole 3-acetic acid alanine; IAA-Asp, indole 3-acetic acid aspartate; IAA-Glu ... Production and hosting by Elsevier B.V. All rights reserved. ..... of free zeatin and dehydrozeatin were below detectable levels,. Table 3 ...
SUMMARY. Abnormal epidermal differentiation and impaired epithelial-mesenchymal tissue interactions in mice lacking the retinoblastoma relatives p107 and ...
Nov 13, 2007 - ... of Biosciences, Cardiff University, Museum Avenue, P.O. Box 911, ...... Tian, H., R. E. Hammer, A. M. Matsumoto, D. W. Russell, and S. L..
Feb 9, 2016 - beta chain (TUBB2A), F-actin capping protein subunit 2 (CAPZB), thymosin beta-4 (TMSB4X), actin-related protein 3B (ACT3), and tropomyosin ...
Mar 10, 2015 - 2 Department of Medicine, Roswell Park Cancer Institute, Elm and ..... BAD. ST. TT. PDX 12424. pCREB/CREB. pBAD/BAD. 0. 1. 2. 3. *. ** ..... and β2-adrenergic receptors (Santa Cruz Biotechnology; sc-567, sc-81578). Patient.
May 23, 2011 - Furthermore, activation of HIF-alpha or NFkB in fibro- blasts drives a loss of stromal Cav-1 via lysosomal degradation, and activates the ...
Apr 13, 2015 - 2 Mayo Medical School, Mayo Clinic, Rochester, Minnesota, United States of America .... and in the fibrosa endothelium, but not in non-calcified regions or the ventricularis endotheli- um (Fig 1A and ... Asterisks indicate calcific nod
Feb 13, 2017 - Consistent with HSF1 activating protein folding and stress- protective pathways .... domain; LZ1-3 and LZ4; leucine zipper domains. NATURE ...
Mar 14, 2017 - Dane Parker,1 and Alice Prince1,3,*. 1Deparment of Pediatrics ... patient-derived data (Shalova et al., 2015; O'Neill and Pearce,. 2016).
Jan 20, 2015 - by Lipocalin 2 (LCN2) modulation through ATF4. Consistent with the key role of LCN2 in CKD progression, Lcn2 gene inactivation decreases ...
Nov 17, 2014 - Christian Bowman-Colin1,2, Ying Su2, David T. Ting1,4, Kornelia Polyak1,2, Andrea L. ... 5 Brigham and Women's Hospital, Harvard Medical School, 75 Francis St, Boston, Massachusetts. 02115 .... As BRCA1 is much more abundant in ......
Instability Drives Replication Stress, Abnormal Development, and ...
Roberto Bellelli, Valerie Borel, Clare Logan, Jennifer Svendsen, Danielle E. Cox, ... Nye, Kay Metcalfe, Susan M. O'Connell, Gordon Stamp, Helen R. Flynn, ...
Molecular Cell, Volume 70
Supplemental Information
Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis Roberto Bellelli, Valerie Borel, Clare Logan, Jennifer Svendsen, Danielle E. Cox, Emma Nye, Kay Metcalfe, Susan M. O'Connell, Gordon Stamp, Helen R. Flynn, Ambrosius P. Snijders, François Lassailly, Andrew Jackson, and Simon J. Boulton
Figure S1 A.
B.
Pole4 (inbred) +/-
+/+
Pole4 (inbred) -/-
(127)
36.0
(25)
63.6
(50)
-/-
11.5dpc
+/+
161
2.0
4.4 4.2 4.0 3.8 Pole4+/+ Pole4-/-
0.35 0.30
0.70
0.60
0.60
0.50 0.45
Pole4+/+ Pole4-/-
0.55
Pole4+/+ Pole4-/-
0.55
0.60 0.55 0.50
Pole4+/+ Pole4-/-
Pole4+/+ Pole4-/-
G.
F. 90.0
Pole4+/+ Pole4+/Pole4-/-
80.0
40.0 30.0
n=16
Pole4+/+ Pole4-/-
-/n=16
10000
I.
5000
20.0
0.40
p=0.01 15000
50.0
0.45
H. +/+
70.0 60.0
0.50
20000
Time on rotarod (ms)
Mice (%)
0.25
0.65
0.65
0.55
Pole4+/+ Pole4-/-
0.30
Pole4+/+ Pole4-/-
Humerus/femur length
0.40
0.35
Pole4-/-
0.6
Pole4+/+ Pole4-/-
Pelvis/femur length
Tibia/femur length
0.45
100
0.40
0.7
1.4
0.65
0.50
(25)
0.8
1.6
1.2
0.4
Pole4+/+
Radius/femur length
3.6
253
p5 gH2AX foci (** p< 0.01; *** p< 0.001); Error bars ±standard deviation (SD) are included; Scale bar, 30µM. (C) Left: representative 53BP1 immunofluorescence staining of CTR1 and POLE1 mutant (P1 and P2) cells. DAPI was used for nuclear counterstaining. Right: Bar graph showing percentage of CTR and POLE1 mutant cells with >3 53BP1 foci (** p< 0.01; *** p< 0.001); Error bars ±standard deviation (SD) are included; Scale bar, 30µM.
