Intestinal deletion of leptin signaling alters ... - The FASEB Journal

The FASEB Journal article fj.14-255158. Published online June 13, 2014.

The FASEB Journal • Research Communication

Intestinal deletion of leptin signaling alters activity of nutrient transporters and delayed the onset of obesity in mice Annabelle Tavernier,* Jean-Baptiste Cavin,* Maude Le Gall,* Robert Ducroc,* Raphaël G. P. Denis,† Françoise Cluzeaud,* Sandra Guilmeau,* Yassine Sakar,* Laurence Barbot,‡ Nathalie Kapel,‡ Johanne Le Beyec,*,§ Francisca Joly,*,储 Streamson Chua,¶ Serge Luquet,† and Andre Bado*,1 *Institut National de la Santé et de la Recherche Médicale (INSERM) Unité Mixte de Recherche en Santé (UMRS) 1149, Unité de Formation et de Recherche (UFR) de Médecine Paris Diderot, Sorbonne Paris Cite, Paris, France; †Université Paris Diderot, Sorbonne Paris Cité, Unité de Biologie Fonctionnelle et Adaptative, Centre National de la Recherche Scientifique (CNRS) Unité Mixte de Recherche (UMR) 8251, Paris, France; ‡Service de Coprologie Fonctionnelle, Hopital La Pitié Salpêtrière, Paris, France; §Université Pierre et Marie Curie, Paris, France; 储Service de Gastroenterologie et d’Assistance Nutritive, Hopital Beaujon, Clichy, France; and ¶Department of Medicine and Neuroscience, Albert Einstein College of Medicine, New York, New York, USA The importance of B-isoform of leptin receptor (LEPR-B) signaling in the hypothalamus, pancreas, or liver has been well characterized, but in the intestine, a unique site of entry for dietary nutrition into the body, it has been relatively ignored. To address this question, we characterized a mouse model deficient for LEPR-B specifically in intestinal epithelial cells (IECs). IECLEPR-B-knockout (KO) and wild-type (WT) mice were generated by Cre-Lox strategy and fed a normal or high-fat diet (HFD). The analyses of the animals involved histology and immunohistochemistry of intestinal mucosa, indirect calorimetric measurements, whole-body composition, and expression and activities of nutrient transporters. IEC LEPR-B-KO mice exhibited a 2-fold increase in length of jejunal villi and have normal growth on a normal diet but were less susceptible (P