introduction materials and methods

MONOGENEANS FROM PANGASIIDAE (sILURIFORMES) IN SOUTHEAST ASIA: VI. PANGASrrREMA CAMILLAE N. G., N. SP. (MONOGENEA, ANCYLODISCOIDIDAE), FROM PANGASIUS POLYURANODON PARISFLLE A.·· ..·. EUZET L.

Summary: The xominolion of glll porosite, from Pon90SlU~ polyuronodon Bleeel .. 852 ISillJll[orme& Pongo.sildoel ln Indone,io reveoled Ih" pre, nce ul Cl n w Ou

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Tbaparocleidl/s) ha\'e heen c1escribecl from the fifteen stllclied PClIlp,asills species (P. bocol/rli SallY:lge, 1880: P. djamhal Bleeker. 1H46: P. p,ip,as Cheyey, 1930: P bu meredis Robert~, 1989: P. b)pophlball1?lIs (Sall\'age, 187Hl: P. kil/abalal/p,clIlel/sis Roherts &. Viclthal'anon, 1991: P. krempji Roberts &. Vidthayanon, 1991; P. kl1/1Vil Pouyallcl, Tellgels &. Legenclre. 1999: P. lithosloilla Roberts, 19H9: P. mek!ol/gel7sis Gustiano, Teugels &. Pouyalld, 2003: P l7aslllus (Bleeker, 1862): P niel/I/xYJ/hu isii (Porta. 19(4): P pal/gasius (Hamilton, 1822): P rbeophilils POll)':IUe! &. Tellgels, 2000 ancl P. sahabensis GlI~tiano, Tellgels &: POllyaucl, 2003) from Inclia, Banglae!esh. Inclonesia, i\JaI:lysia, Tl1ailanc.i and Vietnam (see Tripathi, 19'57: Lim, 1990: Pariselle el al., 2001a, 2001h. 2002a, 2002h and 2(03).

MATERIALS AND METHODS ish, callght bl' hook ancl line, were bOllght in fish markets or directl)' from fishermen in IndonesiJ (Kalimantan, South part of Borneo Island). The fish were cli~sected as soon as possible, :11le! the [dt branchial arches were frozen in liquicl nitrogen, until eX:lmination. To \'erify the specifie identity of bost fishes, the carcasses \Vere nllmberee!, fixecl ane! preser\'ecl in formai in. In the laboratory, the gills were thawee! ancl the monogeneans \\-ere detachecl frol11 the

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PARISEtLE A, EL:ZETL &: LAlIBERT A,

DESCRIPTION PASC;ASfTREJ;fA N. G, N,

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Diagnosis: Ancylocliscoicliclae. Three pairs of helcl glancls. Rare ocellar granulae. Haptor with t'\vo p~lirs of gripi. Ventral gripus. morphologically original, with cuneus. Ventral transverse bar absent. Dorsal gripus ,vith dorsal transverse IXlr. Fourteen uncinuli. Vas cleferens arising from anterior region of testis, looping left intestinal hranch, ascencling, f'orming anterior blincl saccular "esicle. Copula tory organ ",ith penis, accessory piece. Ovary anterim to testis. i'vleelioventral vaginal opening. Tubular sclerotised \'agina. Eggs ()\'oiel. Parasites of pangasiiel fishes.

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Comments This new genus is easily distinguishecl from al! others describecl From catfish hosts in ha'-ing no ventral trans\'erse bar, a medioventral vaginal opening, a very special ventral gripus morphology. cuneus associated with ventral gripus (not with elorsal ones as the case in ThajJarocleidus ]ain, 1952, the only other genus of monogeneans clescribee! on pangasiid fishes). Panp,asitremû n. g. is namecl after the host family (Pangasiiclae ). PANGASJTRE\1A CA1;IlLLAE N, G"

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(Figs 2, 3) Fig. 1 - 0le1surl'ments used in this stud)'. DB ~ uorsal trans"erse bar: x ~ total kngth: ". ~ wiuth in the middle. DG ~ dors:11 gripus: :1. b. c. cl ancl e ~ slancl:lrd l11easurements. li ~ tot:1i length of the uncinuli. \'g ~ "agina: L ~ tot:ll lengtlL 1 ~ maximum wiclth. C ~ euneus: L ~ Iength: ) ~ largest width. VG ~ 'l'ntr:1i gripus: T ~ tot:1i iength including fibrous parts: L ~ tot:ll length: e ~ standard l11easurel11ents of the point. ~IA ~ male appar:ltus: Pe ~ total length of the penis: Ap ~ kngth (lI' the :lcceSSOlY pieee: Cu ~ cupule like structure maximum di:lI1lctcr.

gill using a strong water CUITent. The '\vorms were then transfeITeci incliviclually to a slide with a mountecl needle into a drop of ammonium picrate-glycerine (mixture described by Malmberg (957)) The preparation was then covered with J round c()\'er slip Jnd sealed with Glyceel (GURR-BDH Chemicals LIeU. From these preparations, drawings were made of the sclerotised pieces of the haptor and of the copulatory camplex using a camera lucida. Measurements, macle with a cligitiser, in micrometers, and presentecl as the mean ± standare! c1eviation followecl by the range in parentheses, are those proposecl by Gussev (962) (Fig. 1). The methocl of numbering of the haptoral pieces is that aclopted at ICOPA IV (Euzet & Prost. 1981). Terminology is that of Pariselle ancl Euzet (995) and N'Douba ('f al. (1999) )')0--

Type host: Pangasius polvuranodon Bleeker, 1W:;2. Site gills. Type localityl Barito River at Banjarmasin (Central Kalimantan Province, Borneo Is!and, Inclonesia). Material studiecl: 18 inclividuals fixee! and mountee! in Malmberg solution. Type-material: holotype clepositecl at the Muséum National d'Histoire Naturelle (Paris): 21 HG Tg 140. Paratypes cJepositecJ at the Muséum National d'Histoire Naturelle (Paris): 21 HG Tg 141 & Tg 142. Adu!ts 3,03'5 ± 6'52 5 (1434-3,980) long, 348 ± 383 (253-436) wicle at level of penis. Pharynx 156 ± 154 (127-189) wide. Dorsal gripus with blade bent at distal third, poody marked guarcl: a = 29 ± 1 (27-32), b = 23 ± 1.2 (21-27), C = 1 ± 0.2 (1-2), cl = 8 ± 07 (7-9), e = 13 ± 0.8 (12-15), no cuneus. Open V-shapecl dorsal transverse bar 2 : x = 20 ± 1.2 (17-22), w = 3 ± 04 (2-4). Strong ventral gripus with irregular, fibrous parts, each apparent!y serving as attachment of muscles associatecl with these gripi, very smal! aperture: T = 34 ± 38 (2542), L = 22 ± 07 (21-24), e = 14 ± 0.9 (12-1 '5) Ventral

1 See Conclusion. 2 One specimen seel11s to ha"e no uorsal transn'rse bar. ",hile the Ilaptor :mu atller sclerotised pieces are in good condition.

_ _ _ _ _ _ _l'arasite 2004. 11. 149-1')2

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