Metformin potentiates the benefits of dietary restraint ...

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30. Thiamin (B1) (mg/Kg). 7. 16. Riboflavin (B2) (mg/Kg). 6.5. 16. Pyridoxime (B6) (mg/Kg). 2.6. 18. Cyanocobalamin (B12) (µg/Kg). 20. 30. Nicotinic acid (mg/Kg).
Supplementary material corresponding to the manuscript:

Metformin potentiates the benefits of dietary restraint: a metabolomic study Marta Riera-Borrull 1,2, Anabel García-Heredia 1, Salvador Fernández-Arroyo 1,3, Anna Hernández-Aguilera 1, Noemí Cabré 1, Elisabet Cuyàs 3,4, Fedra LucianoMateo 1, Jordi Camps 1, Javier A. Mendendez 3,4 and Jorge Joven 1,5*.

Supplementary material and methods S1: Food intake measurement Food intake measurement was performed as previously reported [1]. Mice were group housed and given a known, pre-weighed amount of food in their home cage hopper. Once a week, the remaining food is weighed. Then, new amount of food is administered. Calculations were performed as follow: Food intake = Food provided – Food ingested – food wasted. Food intake measurements were divided by the number of animals per home cage, so assuming a meanintake per animals, and represented in g, although it can be easily converted to kcal, by using data on Table S1. [1] Ellacott KLJ et al., Cell Metab, 2010.

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Table S1: Nutritional composition of experimental diets.. CD (Chow diet)

HFD (High-fat diet)

16.1 3.1 3.9 71.8 4.2 43.5

24.10 34.60 7.2 24.80 8.5 23.30

3.3 (13.97) 19.3 8.4 72.4

5.70 (24) 19 60 21

Calcium (%) Phosphorus (%) Sodium (%) Magnesium (%) Potassium (%) Chloride (%)

1.38 0.99 0.47 0.34 1.45 0.69

0.98 0.65 0.20 0.17 0.98 -

Caprid acid (C10:0) (%) Lauric acid (C12:0) (%) Myristic acid (C14:0) (%) Palmitic acid (C16:0) (%) Palmitoleic acid (C16:1) (%) Margaric acid (C17:0) (%) Stearic acid (C18:0) (%) Oleic acid (C18:1) (%) Linoleic acid (C18:2) (%) Linolenic acid (C18:3) (%) Arachidic acid (C20:0) (%) Paullinic acid (C20:1) (%) Arachidonic acid (C20:4) (%) Cholesterol (mg/kg)

ND ND ND 0.87 0.03 ND 0.08 0.53 1.34 0.15 ND ND ND ND

0.04 0.07 0.44 7.72 0.94 4.34 13.57 4.75 0.51 0.02 0.01 0.53 265

Lysine (%) Methionine (%) Cystine (%) Met+Cys (%) Threonine (%) Tryptophan (%) Arginine (%) Histidine (%) Valine (%) Isoleucine (%) Leucine (%) Phenylalanine (%) Phe+Tyr (%) Glycine (%) Glutamic acid (%) Aspartic acid (%) Proline (%) Alanine (%) Serine (%)

3.9 1.49 1.08 ND ND 0.88 4.75 ND ND ND ND ND ND 0.41 ND ND ND ND ND

1.98 0.83 0.46 1.28 1.07 0.31 0.88 0.76 1.64 1.25 2.36 1.29 2.57 0.50 5.41 1.79 2.76 0.79 1.43

Vitamin A (IU/kg) Vitamin D3 (IU/kg) Vitamin E (IU/kg) Vitamin K (as menadione) (mg/Kg) Vitamin C (mg/Kg) Thiamin (B1) (mg/Kg) Riboflavin (B2) (mg/Kg) Pyridoxime (B6) (mg/Kg) Cyanocobalamin (B12) (µg/Kg) Nicotinic acid (mg/Kg) Pantothenic acid (mg/Kg) Folic acid (mg/Kg) Biotin (µg/Kg) Choline-Chloride (mg/Kg) Inositol (mg/Kg)

7500 1000 120 2.5 ND 7 6.5 2.6 20 75 17 0.5 40 1600 ND

15000 1500 225 20 30 16 16 18 30 45 55 19 310 2300 80

280 90 64 18 ND ND ND

139 82 56 12 0.97 0.13 0.13

Crude Nutrients Crude protein (%) Crude fat (%) Crude fibre (%) Carbohydrate (available) (%) Crude ash (%) Nitrogen free extracts (%)

Energy Energy density (Kcal/g (Kj/g)) Calories from protein (%) Calories from fat (%) Calories from carbohydrate (%)

Minerals

Fatty acids

Amino acids

Vitamins

Trace elements Iron (mg/Kg) Manganese (mg/Kg) Zinc (mg/Kg) Copper (mg/Kg) Iodine (mg/Kg) Selenium (mg/Kg) Cobalt (mg/Kg)

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