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Methods for Characterizing the Co-development of Biofilm and Habitat. Heterogeneity. Xiaobao Li1, Jisun L. Song2, Alessandro Culotti1, Wei Zhang1, David L.

Journal of Visualized Experiments

www.jove.com

Materials List for:

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity 1

2

1

1

3

1

1

Xiaobao Li , Jisun L. Song , Alessandro Culotti , Wei Zhang , David L. Chopp , Nanxi Lu , Aaron I. Packman 1

Department of Civil and Environmental Engineering, Northwestern University

2

Department of Chemical and Biological Engineeering, Northwestern University

3

Department of Applied Mathematics and Engineering Sciences, Northwestern University

Correspondence to: Xiaobao Li at [email protected] URL: http://www.jove.com/video/52602 DOI: doi:10.3791/52602

Materials Name

Company

Catalog Number

Comments

Peristaltic Pump

Gilson

Miniplus 3

Flow cell setup and inoculation

Pump Tubing 0.50 mm OVC, Orange/Yellow

Gilson

F117934

Flow cell setup and inoculation

Three-way Stopcock w/ Swivel Male Luer lock

Smiths Medical

MX9311L

Flow cell setup and inoculation

Sylgard 184 Solar Cell Encapsulation for Making Solar Panels

ML Solar LLC

Pyrex Medium Bottle, 1 L, GL45

VWR

16157-191

Flow cell setup and inoculation

C-FLEX Tubing

Cole-Parmer

06422-02

Flow cell setup and inoculation

1 ml TB Syringe

BD

309659

Flow cell setup and inoculation

Polymer Tubing

IDEX

1520G

Flow cell setup and inoculation

Sterile Intramedic Luer Stub Adapter

Clay Adams

427564

Flow cell setup and inoculation

PrecisionGlide Needle

BD

305195

Flow cell setup and inoculation

Spectrophotometer

HACH

Syringe filters - sterile (0.2 μm)

Fisherbrand

MAXQ Shaker

Thermo Scientific

Ammonium sulfate

Sigma Aldrich

A4418

Growth media

Sodium phosphate dibasic anhydrous

Sigma Aldrich

RES20908-A7

Growth media

Monobasic potassium phosphate

Sigma Aldrich

P5655

Growth media

Sodium chloride

Sigma Aldrich

S7653

Growth media

Magnisium chloride

Sigma Aldrich

M8266

Growth media

Calcium chloride

Sigma Aldrich

C5670

Growth media

Calcium sulfate dihydrate

Sigma Aldrich

C3771

Growth media

Iron(II) sulfate heptahydrate

Sigma Aldrich

215422

Growth media

Manganese(II) sulfate monohydrate

Sigma Aldrich

M7634

Growth media

Copper(II) sulfate

Sigma Aldrich

451657

Growth media

Zinc sulfate heptahydrate

Sigma Aldrich

Z0251

Growth media

Cobalt(II) sulfate heptahydrate

Sigma Aldrich

C6768

Growth media

Sodium molybdate

Sigma Aldrich

243655

Growth media

Boric acid

Sigma Aldrich

B6768

Growth media

Dextrose

Sigma Aldrich

D9434

Growth media

Copyright © 2015 Journal of Visualized Experiments

Flow cell setup and inoculation

Flow cell setup and inoculation 09-719A

Flow cell setup and inoculation Flow cell setup and inoculation

Page 1 of 2

Journal of Visualized Experiments

www.jove.com

Luria Bertani Broth

Sigma Aldrich

TCS SP2 Confocal Microscopy

Leica

SYTO 62

Life Technology

S11344

Fluorescent imaging

Cy5

GE Healthcare Life Sciences

PA15100

Fluorescent imaging

Red Fluorescent (580/605) FluoSphere

Life Technology

F-8801

Fluorescent imaging

BioSPA

Packman Lab

Image Processing

ImageJ

NIH

Image Processing

Volocity

PerkinElmer

Image Processing

Streams 2.02

University of Cantebury

Image Processing

Copyright © 2015 Journal of Visualized Experiments

L3022

Growth media Fluorescent imaging

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