Microsoft Word - Additional File 1.docx

2D structure “Identical residues”* Caenorhabditis_elegans_1 5 Caenorhabditis_brenneri_1 5 Caenorhabditis_briggsae_1 5 Caenorhabditis_remanei_1 5 Dictyocaulus_viviparus_1 1 Haemonchus_contortus_1 1 Ancylostoma_duodenale_1 1 Ancylostoma_ceylanicum_1 1 Loa_loa_1 1 Wuchereria_bancrofti_1 1 Brugia_malayi_1 1 Toxocara_canis_1 56 “Identical residues”* Homo_sapiens_1 670 Monodelphis_domestica_1 694 Chelonia_mydas_1 658 Gallus_gallus_1 657 Xenopus_tropicalis_1 669 Latimeria_chalumnae_1 617 Maylandia_zebra_1 659

H1 TPR1A H2 H3 TPR1B H4 TPR2A H5 TPR2B ------HHHHHHHHHHHHHHH-----HHHHHHHHHHHHHH------HHHH.........--...HHHHHHHHHHHHHHH..HHHHHHHHHHHHHHHHHHHHHHHHHHH--HHHHHHHHHHHHHHHHHHHHHHHH..------------.HHHHHHHHH * * * ** * * * ** * * * * * * * * * ** SVDKQHIEKLDELRKNVSCTV-----IGFAEQTAELQQEI------SELFIAEFGVNGP--IDMNSLSKLARITSYYASSEYFQGLAKYQRTACKMFITWQTLRKEAM--ECRSKDREIFASIPAKLCFFYFYNGE------------LCRAVVCLLD GPNKEDKIKLDSLIKDVNTYV-----KSFVEQVDDLQQYV------AELFIREFGENGP--IDLNSLNKLARITSHYASSEVSQKLGRYQRAIQKLFTTWQSLRTEAL--ECTNREKEQIAVIPAKMSFFYFYNGE------------LCKAVVCLLD DPNKQDIEKLNELYNSANTII-----HSFLGKIDEIQREV------ADLFISEFGVNGS--IDLHSLGKLARITSQYASFECSQKLGKYQLSVQKFFNAWQSLRKEVA--ELSTKERVRYALIPAKICFFYFYNGE------------LCKAVVCLLD SSTKRELDDLEILIKDVNSII-----TSFVEQTVELQQHV------ADCFLSEFDIHGS--IDLRAITKLARITSRYASSESFQNLGKYQRITQKLFNAWQSLRKDAL--ECSSKYKASIAVIPAKLCFFFFYNGE------------LCKAVVCLLD --------------------------------MPKHIIKV------FISLKDEFTHENV--CEPTSSPKYMTVVSTFASIAAAR--GDIYGNLNKCYTIWRDKIEPNV---RNSTKKHTYAELPLRLAACFLLNGD------------YYKALSCLLH ---MKSSIDKRVVYNNCLQYL-----NLYIDGLVAVQEEV------FNALKNDFKPEDV--CEPTATPKYLTVLSKFTSSSASK--GNLYENVIKCYTIWKDKLEPVV---RDATKKSAYAELPLRLAACFFLNGD------------YYKALLCLRH MSKKAPSISKRDVYRNCLEYV-----RRYLVDLASVQEEV------FANLKEEFKPEHV--CEPTASPKYLTAITKFT-SVHNK--TKIYSSIKKCYHIWKDKLQPVI---GDGGMKNSYAELPLRLAACFFLNGD------------YYKALLCLRH MSKKASSVNKRDVYRNCLEYV-----RRYLVDLAVVQEEV------FANLKEEFKPEHV--CEPTASPKYLVAITKLA-SAHNK--VKIYSSIKKCYHVWKDKLQPVI---GDGGVKNSYAELPLRLAACFFLNGD------------YYKALLCLRH -MVREGKRQLDSMKKELLLHM-----KNHIVATMSARREI------YDALKELFDTNSADFYNLSSTVKHVRIIRLLI-KCLEK--YKPHQHLQDAFFVWNSRLAPAVLAIADPAEQRKYSRHAKLLAACLLDSDE---------ACFYTKAIICLGH -MIREGKPQLDSMKKELLLHM-----KNHIVATMNARREI------YDALKESFDTSSADFYNLSSTVKHVRIIRLLI-TCLEK--YKPHQHLQDAFSVWNWRLAPAVFALSDPVERQKFSKHAKLLAACLLDSDE------------YTKAIICLGH -MIREGKPQLDSMKKELLLHM-----KNHIVATMSTRREV------YDALKESFDTSSTDFYNLSSTVKHVRIIQLLI-TCLEK--YKPHQHLQDAFSVWNCRLAPAVFALSDPVERQKFSKHAKLLAACLLDSDE------------YTKAIICLGH IEVKYQIERVDTVDASPFLDLYPLV-DELFVSTQLGQRAISSCTFMFNAVKKE-EDNGFHFFDTTHFETHAALSKLFI-AALAK--HKAHFYLERAYSFWSEQFAPAVLALESKEERTTFAQFPKLLAAYLLVAGE------------YSKVVVCLAH * * * ** * * * ** * * * * * * * * * ** * ARDQLLDDKAQALLWLYICTL-----EAKMQEGIERDRRAQ-----APGNLEEFEVNDLNYED--KLQEDRFLYSNIAFNLAAD--AAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASL--QILAALYQLVAKPMQALEVLL------LLRIVSE ATDQFLDDKAQALLWLYICSL-----ETKMQKGIEQDLRLK-----LQPSSIELETNDLNYED--KIQDDHFLYSSIAFNLAVD--AAQSKCLDQALALWKEMLKKGQVPTVRCLQQTTASL--QILAALYQMVAKPLQALETHL------LLRTLSE NQEQLLDDKAQALLWLYICTL-----ESKMQESIERDQRAGDQ---GHKNLEDFEPNDLNYED--KLQDDKFLYNSISFNLVAD--SAQAKCLDDALALWKKLLAKKEVPVVRSAEQTVFSL--HFLAALYRMMAKPLQSMESYL------LIRTLSS NREQLLDDRAQALLWLYICTL-----ESKLEEGIAREQRVKAL---GQKSLEDFEPNDLNYES--RLQEDAFLNSGISFNLLTE--LAQSKSLDDALALWKQLLASQGIPAVRSVEQTTASL--RVTAALYRLMDKPLQAMESYL------LVRALCS PTERLQDIKAQALLWQYICTL-----EANMREGLEEQNRKAKLEGQNQWTGVDYEPNDLNYED--KLHDDQSVRDGICFTLAGE--TGPLKGLDEALDLWSSLLSSSQVQYLNSAEQTIFSL--HLLGSLYRLMGKPLQSMQSFQ------LAGRLCH DAEKLLDDKAHASLWLYISTL-----ESTVKKSIEREERVANL-GADLGNLEEQETNDLNYED--QLQDDKFVHDGINFNLAAD--SGQSQYLEDALVLWKKLVPEKRLPQVRSTDQTMASL--HIMASLYRLIGKPLQAIESYF------LIVRLVK NADKLKDDKAHALLWLYICTL-----EKNLQEAIDSDRKRKELREQTQCVTSSIGNNDFDYEDKQKTQDSIPVYEGLHFNLTAE--HKLCQPLESALDIWTTIFQSETLPSVREPKETCRSI--GVTASLFRLMGKPLQALKALQ------ILTDFSR

804 827 797 796 809 756 803

Limulus_polyphemus_1 Nematostella_vectensis_1 Cephus_cinctus_1 Acanthaster_planci_1 Branchiostoma_floridae_1 Priapulus_caudatus_1 Lingula_anatina_1 Crassostrea_gigas_1 Lottia_gigantea_1

QMVQVKLQLASSYFWLYETRV-----QELQKASQEEVKKSP-IISLEDKQEANQLP-RGDEDD--KDDVCDINPAYPHVNLQLE--IESLKPLDSALDLWKDVFLT-(7)--IIKD-KQIIEYIKCAAESYAVLQHVYQEIQALL------VLQQSSH -SFAGVQEELGAAFLLQSIAQ-----HGSTMRE-GPSRRHEPLNTSDADGTGVSDG-------------------QEEQQVALN--QAYIQSLHAAVDAWTAMVDS-(13)-CLRNPLETINSVRQASVMYALLNQPLNQAHALH------LFALIAT HTWSHISLVAGFEFYKFINHA-----RTMSKQTQCEMDSA--KFALLAPKLNSTQ----------EPE--NVVPAYSTINVKED--SRLQNYLQQPLKTWALYIENIDTLESFVPWEFEYTLQTIVIAGEYCRLHRFLDCEINAWK-----LAYNLAT DYLVVFDLLATAHFWLYICQS-----EASQATYITSQFA-----NTKGESTRADCPEPAT---IKKDKASSYLLSTSGYTLATE--HSATEPLDRALDIWMDLVAEGCSQSMF-NDPACTSYCLKVAASLCELADRPIQQVQALN------LLVVLSI STTTLHDDLATAYMWLYMCKQ-----QENLAQVPKLDSS-----QKQSDKEKDKDSQLTYTENETSLNKLLSNISLQDCQLGPE--EVAMAYLDHALENWCLVIREGLDLSAV-RTVESTAKCLKITSSTYGLAGRPYHQARTLH------LLATLME ASLIAKHHLASAHLWLYVMQS-----QARQEQAAAKTVA-----TSGGTPTGANDD--------GEDVASFDERTSSHVTLQQE--REMTGALRAALALWRDIACATDDDLRRDLRVTDLLRNLELLAAMFELILEPVLQVETLV------LVHRVAT TLSILKDVLARAFFWLYVCEA-----ESITTESQSNLGAP-------DEAVERAGS--------PTPEEENCVLNGPVFTLHME--NQAIANLDKALDLWSAILPDGCRADCV-RDCVVTSEGLLQVAQLYKMAGKPVQALRALS------AASHLGD SGGEEMLVLADVHLWIYIVQH-----ERLCKQLISSSQV-----LSKGEESNPDSG--------EEPQPSGALVFEEMCTFSME--MEIIRRLDQALELWENVPNR--------EHTESSLRSVMLCSSILRLAGKVEREQRALF------LALGVCS YSGIDKIILGQIYLWKYIQLH-----ETTCQKFLLGKSEP---------VVIDNDD--------ADDENDDDNKTRQLYSLSDE--EEMIKLLERSVKLWLEAYHTPLSDT---IQKHSFIESLKLTAAIYRALKKPCKESILYS-(24)-VLLMLKS

793 900 797 886 836 527 826 791 786

653 770 666 751 698 396 698 668 638

2D structure “Identical residues”* Caenorhabditis_elegans_1 Caenorhabditis_brenneri_1 Caenorhabditis_briggsae_1 Caenorhabditis_remanei_1 Dictyocaulus_viviparus_1 Haemonchus_contortus_1 Ancylostoma_duodenale_1 Ancylostoma_ceylanicum_1 Loa_loa_1 Wuchereria_bancrofti_1 Brugia_malayi_1 Toxocara_canis_1 “Identical residues”* Homo_sapiens_1 Monodelphis_domestica_1 Chelonia_mydas_1 Gallus_gallus_1 Xenopus_tropicalis_1 Latimeria_chalumnae_1 Maylandia_zebra_1 Limulus_polyphemus_1 Nematostella_vectensis_1 Cephus_cinctus_1 Acanthaster_planci_1 Branchiostoma_floridae_1 Priapulus_caudatus_1 Lingula_anatina_1 Crassostrea_gigas_1 Lottia_gigantea_1

137 137 137 137 101 125 127 127 134 131 131 197

805 828 798 797 810 757 804

H6 TPR3A H7 TPR3B H8 TPR4A H9 TPR4B H10 TPR5A H11 TPR5B HHHHH-..HHHHHHHHHHHHHH....HHHHHHHH......HHH------------HHHHHHHHHHHH.----------.HHHHHHHHHHHHHHHH-HHHH.....HHHHHHHHH---HHHHHHHH................HHHHHHHHHHHHHHHHH * * ** * * * * * * * * * * * * * --YIDLS-DDTLAKEAALRWLMFLGETELIEKKLKTWKMDKSSKD------------MFSATEFAMNYLK----------KSEYRVEMLEKLMKLRD-KVKSDPTRSFSRYELASY---VSWLCSTLSNVPVGSALRECEFPDRVSHIQEAALKSDSLVR --YVELVPEDVLTMEATLRWLMFLGETELAEKKMKQWKTEKSSED------------IFKATRIEIGFLK----------HGDDRVELINELIALRD-RIKAENIRSFPRYELASY---VAWLCSTLSNSAVGASLTGLEFPDRLSQVQEAVNKAESIVR --YVELVPEDAMSMEAALRWLMFLGETELAEKKMKSWKMAKNSAD------------VFEATRIGISYLK----------TTESRSAALDQLIQLRD-RIKSENIRSFPKYELSSY---VHWLCSTLSNVAVGTSLSGCEFPDRMSQVHESSSKAEAIIR --YIELVPEDVLTMEAALRWLMFLGETELAEKKIKQWKMEKSSED------------LFEATKIAISYLK----------KSDNRVDMLKKMIDLRD-KIRAENVRSFPKYELASY---VYWLCSTFSNVPVGKTLNGCEFPDRMSQLQEASSKAEAIIR --SINLESKNILPRVLALRWSAFLGEWEMAEMALAFENAKFMQKD-VEDYFASHLILTKEFVKYYVDFNR----------DHNTRSNSGTSLLKMTH-KINADTRTTFLTFEAQSF---ANWIY-------------------SFNCLHRAVVKSEGVMK --TIYLEPKNLVPRILALRWSVYLGEWEMLEMALAFEKLKPPKSHGDDDFVMAQLRFIIEIAKSYVEFNR----------DHKSRPTSAKSILEMTN-LVNSDTRMTYYIFESQAF---ANWICAQLP-KVAKFSTDD-PLLDSYNCLHRAVSKSESVMK --SIHLEPKSLYPRILALRWSVYLGEWEMAEMALAFEKAKPPQKNGVDDHLVEQLVFMTEMVRAYVDFNR----------DQRSRAVSAKTILTMTE-KTNAEERPTFPIFETQAF---ANWVAAHLP-KVAGFRVENLELFDTFNCLHRAVLKAESVMK --SIHLEPKSLYPRILALRWSVYLGEWEMAEMALAFEKAKPPQKNGVDDHLVEQLVFMTEIVRAYVDFNR----------DQRSRAVSAKTILTMTE-KTNAEERPTFPIFETQAF---ANWVAAHLP-KVAGFRVENLELFDTFNCLHRAVLKSESVMK --VVRLNEGDCHSRLLLTYWLCLLGYWSLAKTQVLCEEEVKRMYSIHKEI-------INEVINDIIHLHTGKTVGHYYGDALSEQDHILKTMQSFES-LLAKSKAKTFQQYEAQILVKRILIAANHFPYADLT------LIGDPLQNMDFVKARYETLVK --AVRLHDGDVHSRLLLTYWLCFLGYWSLAKTQVLDEDEVKRMYSIHKEI-------INEVINDIIYLHAGKAAGHHSGDSLPAHDRILKTMQSFES-ILTKSKAKTFQQYEAQILVKRVLIAANHFSSADLT------LIGDPLQNMDFIKARYETLVK --VVRLHDGDVHSRLLLTYWLCFLGYWSLAKTQVLDEDEVKRMYSIHKEI-------INEVINDIIYLHTGKAAGHHSGDSLPAHNRILKTMQSFES-ILTKSKAKTFQQYEAQILVKRVLIAANHFSSADLT------LIGDPLQNMDFIKARYETLVK --AIRLNEFDPTCRILILRWLCHLGEWQLAKKQLDIEAKLPPISGTHYD-------LIIDIYRNIVALNT-----------LSKKNEVVEKLVMQWN-RLSEEGSKTFMQYQCQALLKRALFIASRLPHADIN------IIGDPLKNSEMEIARLTVLIK * ** * * * * * * * * * * * * * --RLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTY-----LLLSLTCDLLRSQL-------YWTHQKVTKGVSLLLSVLRD-PALQKSSKAWYLLRVQVLQLVAAYLS--LPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLR --NVKDYIRAASASCHITRLLLTLDCPGYAQFYLEEAESSLQLSDHTSDGY-----LLHTQTCALLRSQL-------YCLQKKVTEGLSLLLSVVRD-STLQKSSKAWYLLRVQALQVVAVYLS--LPSSSLSVQQWEQLYTQGWQTSEIALIESHKLLR --VLGDWLGTANALCQVTKLLFLLESPSEAKIFLEEAESCLQKADCSSDSY-----LVLKQTCLILHSQL-------CCANHKVEEGLTLLLEVLQH-PALQKISKVWYLLRAHVLQLMAVYLS--LSSTSLLPELRQKLWAQGWKTPETALADAHKLFR --ALGDNLGTAGALCQVTKLLLQLECPSYAKLFLEEAESCLQRTDGGSDSY-----LLLQQTCLVLRSQL-------YCASHRIKDGTALLLEVLQN-PALQKTAKVWYMLQAQVLQLTATYLS--LPPSHLSPEFRQYIFTQGWKTPETALSEAHRLLR --SLQDHIKEVGALCHLTKILFYLESPEYAQVILQKAEEILKRADRTSENY-----TLAELSCQLLRSHL-------CRVTRQVKEGVELLLGLLQH-PSLQKSSKAWYLLKVQVLQELSLFLM--LPLENLTSDLYRQLWAHGCHNPETALTEAHKLLR --ALSDDLPLVNALCQITKLLLTLGCPSAAQGYLDEAELWLQSADALSDGY-----SLMKLTCAVLRSQL-------YCSSQ-----------------------KLWGNVELRHL----VYET--APSS--------------WKMQEAALLDAHKLLC --KLADNEACASSLIHSASILLDLGSTELAQTQLDQVESVLPSN--TAEGS-----SSLSLLAILVKAQC-------HYNNGQIDCGLRCLIEVLKQLKQQQKQSKSWYLLRARTLQTCASFLS--IDAALLPQVQKNLIMEQGISTSDGALYESLKLLC

794 901 798 887 837 528 827 792 787

-LLNNQSEEIYSISRLVHVLSKFRMLDVAETLVKRGEDLLKEITDAKLLS--LQLLPLLQSRFTLAYLEF-------LYHSGKFDTGRELLKRCESS-PLWEKNTKTTMLLQAEA---KLVACLFNLLPSSAFSSSSIQ-RGDCASKNKLFPENPIVLAL 938 -ALGTPEAMSDA----VVAYAQAVHSLCSIQDVYHARQMVRRMEEAAVCK----PTPLANIHLMLARSGY-------LLLVGKFSEGSECLRSVLSS---EILSKKTASSYVVLA-TARVLQAQYMALCPEMLTPPT----PSGVSSVHHQPATPLECLS 1036 -KQKNHRVIIYV------VGRAILSRYINKEWIDTATKLSIDLKHSTDNN-VIDAIVT--YWIGLSDF---YFEIGKHEEASKLFDEATILQESSILCNQSTYLMRLDILLRNPK---------------------------LSIRSNETYNFSLYIVQS 917 STSSNEDTVLAY--CQIIQALCRMGAVEEATALLAPAQALMGEAETE-GEDGAGLQVAFTIAKCYVVLAAHKTGEFETLLEKVLQIHSHK-RRSRQAYLRNASIKHLQALYLLRPPSERSQD------------ASVLRGLVPDDLSPLELQAEAMRLQM 1030 SAQDYEEAVFTI--ATATIAMCLHGNSCHGDQLLTKADSLVGKLEDN-----SRALLLLRVAKSHFLLLTGEVSTGWSLLCEVLSSPILE-ECSTICYMLSAQARHVQSLYLRLPLGQAHMD-------------------RAEGSSCFETAFDAFRVRL 969 ACCESARAVVLTNAANLVRALCSVGLVDVAAAVGEKVEAGAEKEKDTEQKETKVARLTWQVAHSEVCYLNEQFDRGWTLLSRVLCCPLLE--------------NKLCSSYMIGSSAALLHSRYKALPARTFLHQSTLASLREDDSSPLELGEKGLRTVI 673 HISFALRVSLHT---LQIQLLCSLNQPQSAKVLMDQVDGWLEKWKPD-RSSEEYVVWKYRVAVAEVMVLSQKFSEAQEIFEMVQQQSLVS-ARTRDWCIVEGTMKRCLAKMLTLPPYHAAV--------------------GECGDYALDRMHEACSLLT 961 --ELGDEAINNIIKCRLSHIYGTHGMHHQARQLLPAIPDQQTGLDDG--------YLQTAMSSVMHYLHTKQVEKGLQLLTQVNDTIGQQPSQSKTVCLANGTVKRCWSLFLSLPMERDPEG------------------GQRSEDYSLDHAIEAYRNHV 923 VVKGDDIAVFQA-NMDTVELLTSLGLCDTSLHILSSIPLPTSKNDQI--------LYKIRQIEASLSIIQKQSE---EMLLELENNLSSQ--KETTACFLRGTTNRLLSTLSGFSLGDNSTD----------------------LHYALDHGHDAVRLHT 910

138 138 138 138 102 126 128 128 135 132 132 198

268 269 269 269 225 267 270 270 278 275 275 330 947 970 940 939 952 859 945

2D structure “Identical residues”* Caenorhabditis_elegans_1 Caenorhabditis_brenneri_1 Caenorhabditis_briggsae_1 Caenorhabditis_remanei_1 Dictyocaulus_viviparus_1 Haemonchus_contortus_1 Ancylostoma_duodenale_1 Ancylostoma_ceylanicum_1 Loa_loa_1 Wuchereria_bancrofti_1 Brugia_malayi_1 Toxocara_canis_1 “Identical residues”* Homo_sapiens_1 Monodelphis_domestica_1 Chelonia_mydas_1 Gallus_gallus_1 Xenopus_tropicalis_1 Latimeria_chalumnae_1 Maylandia_zebra_1 Limulus_polyphemus_1 Nematostella_vectensis_1 Cephus_cinctus_1 Acanthaster_planci_1 Branchiostoma_floridae_1 Priapulus_caudatus_1 Lingula_anatina_1 Crassostrea_gigas_1 Lottia_gigantea_1

948 971 941 940 953 860 946

H12 TPR6A H13 TPR6B H14 TPR7A H15 TPR7B HHHH.........---------..........-------........-------HHHHHHHHHHHHHHHHHHH..........HHHHHHHHHHHHHHHHHH...HHH---------------HHHHHHHHHHHHHH..........HHHHHHHHHH * * * ** * * * ** NRIPGLASSQFDN---------SVNASIWPFL-------DGHQED-S-------NYYVHIGSTIAWHFEMRRE-CALVNVTTAQTRDSMSAMILNLRVALKSASFFRV---------------LQTTNTLAYYSSIIEEAGSEKNAKLMRVSCVNLL NRIPGLAAYQFDN---------SVNSSIWPFL-------EGSRNGSS-------TDYIHLGSTLAWYFEMRRE-LALVNVATAQTRDSMSSMILNLRIGLKSASFFRI---------------LQTTNLLAYYTALVEEVGSERNAKLMRVSCFNLL NRVHGLAAYQFDN---------SVNASICQFL-------NEGSHG---------TDYVHLGSTVAWHFEMRRE-LALVNVAAAQTRDSISAMILNLRIALKSASFFRI---------------LQMTNVLAYYTGLVEEVGCEKNARLMRISCVNLL NRVPGLAAYQFDN---------SVSTSIWPFL-------EEKHHGSS-------TDYIHLGSTVAWHFEMRRE-WSLVNVSTAQTRDSMSSMILNWRIALKSASFFRI---------------LQMTNLLAYYTTIIEEVGTEKNAKLMRISVVNLL NRIPGIHKESE-----------SIDFR--------------KVTCDT-------TDFVKACASYAEFCELRRE-YSIELLNVGMVHDAAFHSQLGLWSALKIGVFFRI---------------QQFVNVNTLIRQCVVQVEFRKDLQFCMNIIRILY NRLPGIQKEHL-----------PMDFK--------------CVARDP-------LDFMKACSSFAECCDLRRD-YVIELLHVGTIRDAAANSQLGLYCALKSGVLFRI---------------QQFVNTNTLLRSCVVHPDFKNDLHNCLDMLRMMY NRMQGIQKEDV-----------PLDLK--------------KIASDP-------LEFLKACASFAESCEVRRD-YAIELLSVGMIRDAAAVSQLGLWCAVKAGVLFRLIKQLAVDASHPTSTILQFVNINTLIRACVVQQEFKKDLRTCMDIMHTIY NRMPGMQKEDV-----------PLDLK--------------KIASDP-------LEFLKACTSFAESCELRRD-YAIELLSVGMIRDAAAVSQLGLWCAVKSGVLFRI---------------LQFVNINTLVRACVVQQEFKKDLRTCLDIMRTIY CRSSVYYGSDMKEEDAGCAAFAGGEFQETKLMSTVSSKLQPQFINIF-------DEYLKLSAVLAEHFKVVLQ-LCFKYIELGILRESESEGLKLLHQVLKTTNLQRC---------------LAVLNVLFLTSTLAHGNSVNVRPDPFRPVIASLW CRSSVYYGNDMKEEDVGCAAFAGGEFQETKIDETKI------------------DEYLKLSAAVAEHFKVVLQ-LCFKYIELGILRESESEGLKLLHQVLKTSNLQRC---------------LAVLNVLFLTSTLARGDIMNVRPDPFRPMIASLW SRSSVYYGSDMKEEDGGCATFAGGEFQEVNP-----------------------DDFLKLSAAVAEHFKIVLQ-LCFKYIELGILRESESEGLKLLHQVLKTSNLQRC---------------LAVLNVLFLTSTLARGDITNVRPDPFRPMIASLW NRYQAFFNYGE-----------GIELQ---------------KKVNP-------DDFLKLCSHISEHFEATIL-QCYELAETGVLRECEGIVMSLWRESFRLGSLPRA---------------LIAINLLMMLVMMS--NYMRKREQSLRQVYAALL * * * ** * * * SIILLLMGSDI--------------------LSTQKAAVETSFLDYGCSEKLVCENLVQKWQVLSEVLSCSEK-LVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQC---------------ALFLVLKGE------LELARNDIDLCQSDLQQVL SIIILLMGRDL--------------------LFVQQSAVDAAFLDYGCSEKLVTENLVQKWQVLAEVLGCSEK-LVTLLGRLGTVSEAKAFCLEALRLAMKLQCVRQC---------------SLFLVLKGE------LELARNDLDLCQSDLEQVL SILLLLMGGDL--------------------LSSPKTVTETQFVDHGCSEKLIAENLLQKWQVLADMLVCSEK-LIALFSQVEMVCEAKAFCLEALKLAMKLQAIRWC---------------AGFLVLKAK------LELQRSELELCHSDLQQAL GIVLLLMGSNV--------------------LGSHKSAADIRFIDCGCSEHLVVDNVLQKWQVLADLLGCSEH-LVVLLSRVEVMCKAKAFCLEAIKLAVKLQAVRWC---------------ASFLVLKAQ------LELQQSELELSHWNLQQAL SIVLLLLGNDV--------------------LASPKGSSEVRFVDNGCTHDLVKENLLQKWQVLADLLSCCRK-LVHTLGRMGCVSEAKSFCLEGLRVAKTLQSMRHC---------------ADFLVRKAE------LETLRSETQLCEEDLQYVL SIVILLTGKSI--------------------VPSHKANANIRFMDQGCCRKLVHENLLQKWQVLADLLSCTHD-LVKLFGWVGSVCEAKAFCLEALKLTMKFKLLTQC---------------ADFLVSKAE------LELQRSRSDLCHLDLQQVL SLLVTLVGKGL-----------------YGTTTSSNTNVVSSFINQGCSKKMVSDNLALKWQLLAELLNCSKK-MVSVRSDCGAINDARLQCLEALKLAIKLQTLSQC---------------SELLVMKAE------LELMQGEKDESRTDLDIVR

939 1037 918 1031 970 674 962 924 911

DAWKLCMGLI-----------------------KYLSSAEAQKSYKD----YLSYKPALLHTLLSSTIL-VGQ----LYIDIGAPREARCYLKDTLKVAQEM-----------------------ALAYRSAEFLLTLANLDAVCENINDCKVKLNG DGCTWVEVAATAIFGQSLL---------------DVDSTAVCKVGSD-----ECLRLTTLHILLHCQLQ-LGA----MLTRLGVVKQAAKFLADGLALAGRF-----------------------HLPYRVIEFKIFQSELDIQIHKLDRARSVLLG LYAMISLS----------------------------SNLIETKSESK-------ERILYGYDVLLSSTNRLSL----RLNSLLSFKEISAHLIQRLKISQKL-----------------------GATLRVAECLKNLCFIDLSRMQLDDCEVKLQG GVGRLMFGEGVFGNEQ-------------------SLQGEGFKLAAVGNDSGGNDLEFNQWTVLANVLRSLYD-VGSLYLEQGCTREAKSYLKEGLLLA------------------------DKFMLPRRSVAFLLQLSNLHVQCGQEDDCQASLS SLARLLFGDRFKETDRLVT---------------ASSASNSAKTKRGY--------PHLPWWCCDDFLTSLLD-MGKLYSDQGCVKEAKSYFLEGLEIA------------------------ANFKLPRRCAQFLTMLAEVELHRGLTEDCQLLLK GIAKLMLGRD------------------------AAEAAAGDDDVACV---DRTTRLVTRYRILIDMLCALHQ-AGDFYLHCGLAREAKTYLKEGLTYA------------------------ERFGLTKWCARLRLSLCEIELRSGQPLDCELMLD GVWKFLQGK-------------------------------ASESAS----------LMDKWSIMTEYLDTLQA-TGHMYLDIGESRAAKCYLKEGMILA------------------------QEFNMPRRLSGFLLSLAKLDTLSEKFTDAEAKLN SVVYFLLGKEHWAV--------------------------DNKGLN----------TTEKYDIIQEYLLSLYH-LGQLERLVGDTRSAKFHIKEGWTTA------------------------HFMGLPKWASMFMNEFSIIHILSENSTAAISSLT SVNHFFLEK-------------------------------KSKAGS----------PHQKWSIIIEVLESLLN-LVKLYRYIGDDSMAKCYAREGIKTA------------------------HSLLLPRWCLLFKFELCRIYSITCCLKEAQDVLT

269 270 270 270 226 268 271 271 280 276 276 331

2D structure “Identical residues”* Caenorhabditis_elegans_1 Caenorhabditis_brenneri_1 Caenorhabditis_briggsae_1 Caenorhabditis_remanei_1 Dictyocaulus_viviparus_1 Haemonchus_contortus_1 Ancylostoma_duodenale_1 Ancylostoma_ceylanicum_1 Loa_loa_1 Wuchereria_bancrofti_1 Brugia_malayi_1 Toxocara_canis_1 “Identical residues”* Homo_sapiens_1 Monodelphis_domestica_1 Chelonia_mydas_1 Gallus_gallus_1 Xenopus_tropicalis_1 Latimeria_chalumnae_1 Maylandia_zebra_1

1060 1082 1053 1052 1065 972 1061

INSERT 1 H16 H17 TPR8A .---------------------------------....----.................-.......---........................-----------------------------...........HHHHHHH..HHHHHHHHHHHHHH * * * * * S---------------------------------SNPI----IVRCSTPKETGATSRAHTPMAGSSV---SEKQNTMRPDLADLLGDLELLDEQ-----------------------------SFHPITRSCVCNVCTIYPLHSSFAAEYMMSYAIH S---------------------------------SEPV----IVRCSTPKEPRVPSRAHTPLPGEKI--RPQQQSTMIADESDEFEDIYLL-EK-----------------------------PFHSITRSCSCTVCLHYPNSSTFAAEYMLSHCIY S---------------------------------SQPI----VVRCSTPKETRAASRAHTPLPGEESGNRRSKSDT-LNILADELEELDLYNDQ-----------------------------IFHPITRSCNCNVCLHYPLSTTFAAEYMMAYCIN S---------------------------------ANPV----LVRCSTPKESRGPSRAQTPLPGEKSGYVPHISETMVAPLEDLMEDLDLFDEQ-----------------------------PFHSVTRTCPCNVCLHYPLSCTFAAEYMMSYCIH LKEPSSKN-----------VFGGSKQK--EVLPDDSEM--SLLLTAKSPMGNRLFPASE-------------GQKSVAISIMESFEDLRLE-----------------------------------------------------------------SNGSSMTDM----------LFGCSKKRPPE-VSFSDELTTEIPLPRISTP--SRSPARV-------------GQKSLATTLIESFEELRLETGKTPQRGRSPSRIASKVIDSFHELTLSETVSSLHELSDSCSCNFCWFCRTNYNASFEYWFSSFLY SSDTLLSKT----------SLGCTKKKPPEMLEFVDNI--DLPIPCTSTPIRSKSPGRV-------------SHKSVALSLAESFEELRLESAKTPPRCKSPGRITSKFIDSFHELTLSETICSLHEISEECECNTCELCSSNYNAAFEYWMSSFLY SSENLSSKT----------SLGFTKKKPPEMLEFVDNI--DLPVPCTSTPIRSKSPARV-------------SHKSVALSLAESFEELRLESTKTPPRCKSPGRITSKFIDSFHELTLSETICSLHEISEDCECNTCELCSSNYNAAFEYWMSSFLY NRSRNET------------SVCVDKEM---------------------------------------------QNKKLNLSSKDQKIIDLTEDEPNNLPQANKENMENKL--------------ILEEHVEYCDCVICTLCDINPQMHIEAIFSKMLF NRSKSGI------------SVCTDSER---------------------------------------------LNRELNLS--SQKIIDLSEDEPNNLSQANKENMESKL--------------ILEEHVEYCDCVICTLCGMNPQMHIEAIFSKMLF NRSKNGI------------YVRTDNER---------------------------------------------LNKKLNLSSGSQEIIDLSEDEPSGLPEANKKNMESKL--------------ILEEHVEYCDCVICTLCGMNPQMHIEAIFSKMLF NVPKRSD------------DSSNTAER--------------------------------SPDPGTLA-------------KEPSLAALMLHAFCYGQESGDGEKKEEKIEDD-----------KLESHNEKCTCVVCATCEHDLQLQFDISFAAFLF ** * * * * * FLLESCT------------EFGGVTQHLDS-VK--------------KVHLQKGKQQAQVPCPPQLP----EEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIP----------NFLSHSPTCDCSLCASPVLT-AVCLRWVLVTAGV FLLGSST------------EFEGIAQLPNT-VK--------------RIHPQKGQQEPRIQPGSELS----EEEAFLKGPALQLVATVEKDPGP----SVSSPVLKSKPRPCP----------AFLSHSLSCSCILCTDPALV-VVCLRWLLVSAGA FLLESGTGEVSFTSDLKERNFETKEKQKSE-VKI-------------KPKKGRSKGSKHQGPSAEPA---GEEDGFLKGPSLEFVDTVSRQEK--ESVLTTSPVLKPKKKRCL----------NFLSHSALCSCSLCSDVTLS-AVCLHWVVSYGQW FLLESGT------------EFEADEKQRAP-LKI-------------LPRKGKPEGRKQRDEGSELP---GEDEGFLKGPALEFVAMVSGLEK--ADDLSSSPQLKPKRKRRL----------AFLAHPAACPCCLCSDLTLS-SLCLRWLLCCARG FLMESCT------------DFAAKSKQK-E-VKI-------------KLCKGKPFHKVDITETPPSP----PKDDFLKAVDLHYVETRDLSKAP------ESPTCKPSLEKNL---------PHFLSHTQDCTCPLCSDLVLS-LLCTQWLVAKAEN FLIESCT------------DFDSRGQVQGK-MKI-------------KVKKQKAVRKQTTKTDPTAE----GEDAFIKGPSLRNIATVSRDK---EGALTASPVLKSKVRKQL----------SFLSHQPACWCLVCTDVELS-LACTKWTVVFGEA NLLERST------------DFSDQVQRA-E-VKI-------------KPRKGRPVQRPQSPLP--------TIEDDLK----DILSTRWTSKEPIVKDLSGSPPLKALPHRWL----------SSLTHEPECQCPCCSEPCLG-RATARWAAAQADL

Limulus_polyphemus_1 Nematostella_vectensis_1 Cephus_cinctus_1 Acanthaster_planci_1 Branchiostoma_floridae_1 Priapulus_caudatus_1 Lingula_anatina_1 Crassostrea_gigas_1 Lottia_gigantea_1

1041 1146 1013 1144 1079 779 1053 1020 1002

-VEYILHTSLP----------ATSVNS---------------------EAAREKNELSEGSDE-EDFLVRRPGRSLRGTVREQLLHG-----------------RRETESSPISQQHVKTSIPTQWEHSISCCCNLCQHIKLHSLELEIVGLQAYCL -VASQMAPLFH---------------------------------------------------------------------------------------------------GTTDRLEDLTDVHAVVSHKDTCRCMCCSDPALQAIQIRVLLVLARFF -LEHILDIESI----------ELSMKS----------------------------------KLQTVETSSYSVSSSERMVESVRD--------------------IAQNPASPVLRRKVFAPSDFLLH-KDCTCYKCRNVPYQYLVFASTYIRAQLY KVQQILHL--PEKAAQARWRGASMVTQDERKSSS----------------GSETHEETDDDNFIRTKRISISAECSSTTWRLEDTDTSS----------------------SPWLKAQTVKLPSYHSHPSDCLCASCADVSLHAQDMTSFLRLAQRL SACSVLAS-(46)-EKERKRTQNKKDARRQRKSAGCFGFD----------VLESEGSEDEDDNFIRSKLI------SVSQQLEEDDGIDSDLEDYGK-------------HQPSFPTM--QLPDYVSHNANCTCSVCKDVVSQTSYLDVFLTATECA SVDYTLNA-EPISAPPLVATVTVATATPANPTTAGRKKKAASGKAAGDTGKMLRGCRGGKPSSGATATPF--------TIYTDDASAADDFLISSRPLSSPQGAP-PSNAAASSPALSRQTRRGGAEHAAGCACARCGDVALQLLNALASALRARCL MVHFILNSEVH-TVKPEKQPTRKMDVENEKAEKQQKKKDDCFGFDISDEEDDDSEKGLDFLKRKVL----------------EDPIPSDENDSGNEDPCA-----------SPSLRKHESKLPVYLSHSNKCSCVLCHDFILHPLSMEYIHGLACLS KTCQVFNGSQDTAGENATNRSSKSRKSKTAGSFGFD----------------------------------------------DDVEKEEDSVESSQSPYP----------------------PCWQHLTDNSTCDVCQDNTVKLAVLSYALVTAKFE DIASILYSNKAEKPGLTKLVSYNTNTKNIPLKDMAEYLQRKRSFADDCFGFESIRDAGIDRFSV--------------SDVSTDPLCDDIPDIN------------------------------LPDHDSSCKCIACKDNSLLDLTSKYWFVLAETC

386 388 402 388 334 377 395 380 428 394 394 438

385 387 401 387 333 376 394 379 427 393 393 437 1055 1077 1052 1051 1064 971 1060 1040 1145 1012 1143 1078 778 1052 1019 1001

473 475 491 478 397 507 526 511 513 477 479 526 1170 1189 1175 1162 1170 1080 1163 1147 1202 1103 1260 1244 925 1181 1108 1114

2D structure “Identical residues”* Caenorhabditis_elegans_1 Caenorhabditis_brenneri_1 Caenorhabditis_briggsae_1 Caenorhabditis_remanei_1 Dictyocaulus_viviparus_2 Haemonchus_contortus_1 Ancylostoma_duodenale_1 Ancylostoma_ceylanicum_1 Loa_loa_1 Wuchereria_bancrofti_1 Brugia_malayi_1 Toxocara_canis_1 “Identical residues”* Homo_sapiens_1 Monodelphis_domestica_1 Chelonia_mydas_1 Gallus_gallus_1 Xenopus_tropicalis_1 Latimeria_chalumnae_1 Maylandia_zebra_1

1171 1190 1176 1163 1171 1081 1164

H18 TPR8B H19 TPR9A H20 TPR9B H21 TPR10A --------....HHHHHHHHHHHHHHHHHHHHHHHHH---------......-------.....HHHHHHHHHHHHHHHHHHHHH.....-----HHHHHHHHHHHHHHH....------..HHHHHHHHHHHHHHH...----------.. * * * * * * ** * * ** * * * * ** --------SDFSQLSIKHFNDEFARIRERGMSSQVLM---------HRDSSV-------RPRPNIIQNEIFGMCVIRWLTKKLDSKESAD-----EDTMEIFNNALKIVRYLQQ------RTTDMILAVTQLGRQLEFPM----------EC --------MNFSQDAIKHFNDEFSHIRERGMEFQLMM---------HRDRSV-------RP----------------------------------------------VVKYLNL------RTTDLLLAVTQLGRQLEFPF----------DV --------SDFTQISMKHFNDEFAQIRERGMNAQTMM---------HRDKSV-------RPRPNIIQNEVFGLCVIQWLKRKVDTKQLVD-----TETLEIFKNALKIVKYLNL------RTTDLLLAVQQLGRQLEFPQ----------DC --------SNFNQTSLKHFNEEFIRIRERGMSSQTMM---------QRNTQV-------RPRPNIIQNEVFGMCVCQWLVRKIDSKQYVD-----QESLEIFNNALKIVRYLNI------RTTDLMLAVQQLGRQLEFPQ----------DC ------------------------------------------------------------------MCETFAICVVRWLRRLEEACWNRENAALRLLREKIVQDALKICSYSSL------RTVPYRLAISQLERLQNLPQ----------RL --------ESMSESAIQCLEERFHALRARVAPEQCAV----------LGKKV-------KPRPPSIMCETFALCVVRWLRRLEDSSWDSENLKLKPLRDSIVKSALKICGYSSV------RTVCYRLSISQLERIRKLSR----------CY --------ESMSNESIQCLEKQFQILRARVAVEQRAV----------LGKKV-------KPRPPSIMCETFAMCALRWLRSLEDSEWDHDNSSLKALREQIIASTLK----------------------------------------------------ESMSNDSIQCLEKQFQMLRARVAVEQRAV----------LGKRV-------KPRPPSIMCETFAMCAVRWLRSLEDAGWDHDNSALRALREQIVASTLKICGYSSV------RTIYYRLAVSQLERIRRVLR----------PS --------YRYSSRMFRVLSERILKLPQETALDVIAICSSLQKQ--ATDKAIE------ERRYPLAALDLFLLAAIRWLRRVGEEEK-------PEIIQDVIRESLKLCSTDPI------YFRWQWLTIRQLARTPL--K----------IP --------YRYSSRIFRMFSERILKLSQETSLDVIAICSALQKE--ATDKAIE------ERKYPLEALDRFLLAAIRWLRRVSEEEK-------PEIIQDVIKESLKICLTDPI------HFRWQWLTIRQLARTPL--K----------IP --------YRYSSRMFRMFSERILKLSQETSLDVIDICSALQKE--ATDKAIE------ERKYPLEALDRFLHAAIRWLRRVSEEEK-------PEIIQDVIKESLKICLTDPI------HFRWQWLTIRQLARTPL--K----------IP --------DSFSSHSFQTLATKFRTLETKFARAHSCM-----------------------------------------------------------CNAIGACVDAELCELEPC------HHRWQWLTIRQLEREPMIDR----------TS * * * * * * ** * * ** * * * * ** --RLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQAS---LNHK--TPPSLV-------PSLLDEILAQAYTLLALEGL-NQPSNES----------LQKVLQSGLKFVAAR---IPHLEPWRASLLLIWALTKLGGLS----CCTTQLFAS --ELSSGHQAKGLDMLRSVLRRCPGATHHLAQVLQAT---LSQE--ASPSPA-------PGFLEDLIAQAYAHLALEGI-NHPPDKG----------LWDILETGLNFVASR---TPFLNPWHASLLVTKSLATLSSLS----CCTTQLFGN --ELASGNRAEGLGLLQASLERCMVMTSRFSTMVREA---SQQK--GKKAAIWDRSM--LGLLDNLVANIYATVAAQSMGGHQPEKR----------LWELLEAGLTFLSSRVPCLPGLEYHKASLLLTKAVATIYSLASKHNGCMAHIFSS --ELAAGSAAEGLGLIQATLQRCGTVGPRFTVLLQDK---LGSG--HKAPA--------LRLLDDLVATGYAALALHSLAGLCPEEK--------- LEEHLKAGLSFLASCSLHLPSLGVSKASLLLAKATAAVCRLAAKHGGSVDAVFAS --------NTQSRGLLKSALKRCKALTQRFSGIVQEI---LGKG--VTKPAV-------LGVASELMARVYLAMA-----SQSPSKL----------PEETLEEGLSFVSSR--QATLPKHWRAGLLLAKALCSIYNLAAKHGGCVADMFVQ --EAAAGNHAECRRLFQTSLERCETLAERFAGFIQGT---LAKEKPAKSASLKGKGMPSFGFLDDLCAKIYSKMAAFSL-TPKPEKT----------TWKVLNAGLIFLTSKAVCLPSLEHAKAALLLAKAVALISSLAAKHCCSATELFSP LLQLDPNDTSVSSKLQWATLSRSKTVTAKLGVKLAKL---FPLY--NTGKHI-----SKPSLMQDIVGRVYLHMALSGV-DPSLTKVCA--------IWKILEAGLAFVESTP--SPMLRPVRAGLMTAKAIASLISLAAKNECTPEELFSK

Limulus_polyphemus_1 Nematostella_vectensis_1 Cephus_cinctus_1 Acanthaster_planci_1 Branchiostoma_floridae_1 Priapulus_caudatus_1 Lingula_anatina_1 Crassostrea_gigas_1 Lottia_gigantea_1

1148 1203 1104 1261 1245 926 1182 1109 1115

AVMGSRSRAIRWLK---NILKLVTFLADKVDF----------------------------LLTSAVKMI----------------------------------------------------------------------------------QASAAPKVSVNCLE---GAEVLCNNAVARSKR----------------------------TLDEITNML---RLGVSEPC---ETDKAPRTKSKGKSKKAKKDDSAECPITTA-----VTAKAMYGVYFA-----EVFVLNAEILLSRGKLT ALQHMTSAAMEHFY---GAFKIRQSIFARESQGSLDSTINKM-----DLERHPWEFYYN-MVEYVLFLL---DFSRF-------------------------------------------IKEYKLAK----------------------EE ELQGEPTQALANLRVALDI-GDWSATKTPSLL-------LRSSSCLHND------EGVKQTKGPEMKLARPSRSSGGQVNLHARATTEIHVCIT----RLTLLNGNLSEAEHTIAGGLELSIDENI--------PKAWLHFYRILVSLELKA LSEGLPAVAIATAAASQKIHGKVCMCVSSTMK-------DFSSSLIHGKKKET-----------------KTKKNSNTEHLFAPQLALLHCRLS----EAYLMQENFSKANTNIESGLLLFQGKSRAIH-LHSNTQAHLLYNAALCAILQEA HDQSRDGELLA---------GRFAAARARA------------AAHAHVE------GATATLRDVVVERLGVAPGGAPSGSVLAESGAAYHVVVA----TAQLRGDDIGSAEAELARGQQLLAGHHG-------ELRARLLHLEAVLSIRKEA MEMFAVSECATLVKTGLQF-YNTAFQKSTQT--------LMSKSCDNLY------KGKKEIDKLVESL-------------FLPWSVRFYIQVADTKIETNLQWGPAGRA---LARAASHLNCSTATGLVNLWKERAFVFYLQALVGLKGMA ---IYIQQNLTFEK--LD---RINCICERFILQ------EPSTSKLGRDLPFCCFQDNSELKDVYVEC---------------------LSLIA----ETSLLLGKTEKCAIVIETAQKFFQSKYVSDLVANLIQNKFTVFKAQMCLMRSLS FHNKFYQHAETFLRKILQ---RSSEIRPKLFENLLTIEKRLCESNQMMD------KVPNVFNDILYES---------------------YCLLS----ECSVYLNSTHETQEICQKALDILDSDVRCSV-----EQNLLRARVNMCLVQLQM

474 476 492 479 1 508 527 512 514 478 480 527

2D structure “Identical residues”* Caenorhabditis_elegans_1 Caenorhabditis_brenneri_1 Caenorhabditis_briggsae_1 Caenorhabditis_remanei_1 Dictyocaulus_viviparus_2 Haemonchus_contortus_1 Ancylostoma_duodenale_1 Ancylostoma_ceylanicum_1 Loa_loa_1 Wuchereria_bancrofti_1 Brugia_malayi_1 Toxocara_canis_1 “Identical residues”* Homo_sapiens_1 Monodelphis_domestica_1 Chelonia_mydas_1 Gallus_gallus_1 Xenopus_tropicalis_1 Latimeria_chalumnae_1 Maylandia_zebra_1

1291 1310 1309 1290 1286 1217 1285

INSERT 2 H22 TPR10B H23 TPR11A H24 TPR11B H25 ................--------------...HHHHHHHHHHHHHHHH.......HHHHHHHHHHHHH.---------...HHHHHHHHHHH...HHHHHHHHHHH... * * * * * * * NYSWMRPTIRKPRVKAT-(29)---------FDKERFEKVRLAMRNEMNHYGHILYREWRCRLFAYVGRT---------SRDPWEAAYAWAESTQIGARNAVQSRLEKCKR NYSWMQPIVKKPRSKKG-(30)---------VDKEKLLKIRSEARREMNNYAHILYREWRCRLFPYIGRI---------SNDPWEAAYAWAESTLIGSRNAIQCKLERCRK NCSWMHPIVKKPRVKAR-(30)---------FNKERYDKVCHEIRREMTQYTHILYREWRSRLFPYVGRT---------SKDPWLSAYAWAESTLIGSRNAVQCRIAKCRK DYSWMQPVIKKPRVKAG-(30)---------FDSARYEKIRTEMKLEMNLYGHILYREWRCRLFPYIGRT---------STDPWEAGYAWAESTLIGSRNAIQCRLEKCRK RYDWMEDDTRTNNFMNT-(27)------NSAEQKDLTKGIVDDALKEYYRYSGLLYRDWRFSLCTFLGEY---------ATCPWLAAMMWSESTLLATRQLARHISGKFKG RYEWMKDTSRAGKFMDA-(23)------EVVAQKIVEKNMMKEALSEYASFSNLLYRDWRFPVCTFLGEY---------VSCPWQAAMMWSESTMLATRQLSRFMSGKMNG ------------------------------EEQKKIEEAAIAEANAEYASYSNLLCRSMGSSNDVVRVN-----------------------------------------SHDWMTDPSLTNRVIST-(21)------DSEEQKKAEEAAIAEANAEYASYNNLLCRDWRYPMCTFLGEY---------SKCPWEAAMMWSESTLLTTRQHSRLLLGKMSG KLEWMHLHVSPI--KAG-(41)--NHKTVTNVTPSYIDMEMEEARKDFNSFSHLFYREWRFQTCSYLGQVCAFQYKADWIEPGWSAAYYFNEAMFISTRQLARMIYKKSEE KLEWLRLHVKARLRSPF---------------------------------------------------------------------------------------------KLEWLRLHVSPV--KAG-(33)--NRNTVTNVINNYVDTEMEEARKDFDSFSHLFYREWRFQICSYLGQVCAFQYKANWIESGWSAAYYFNEAMFISTRQLARMISKKSEE HLPWMLPSVSSDRIRLS-(45)--DSETA-NGMNPRYCQLYETANEDFEQYSHLFYREWRYLNCSSLACA--------NLSDNWMSAYFFSEATCFATRQLARTVDEEGES * * * * * * * SWGWQPPLIKSVPGSEP-(262)-PSAPV-ATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLG-------HRDPYATAFLVTESVSITCRHQLLTHLHRQLS TWAWQPPAAKLSQMSKS-(256)-ASTPA-AFGQPTLESIYDSLSIAFRCVSHCPPSELYAQLCRLLALCLG-------HRDPHTTALLVTESMSITSRHQLLTTVHRHLH AWAWKPLTPS-SETKGS-(322)-SSLAA-AVSLSSLDSVCNSLKAAFQAISHCPPGTLYCQLCRLLALCIG-------SRDPLTTAYLVSESVSITVRHQLMSNVHRRLY AWAWQPAPLSPTEPKAA-(361)-NVLPA-VDDASLLDTACGLLKSAFSCISHCPPGTLYSQLCQLLALATG-------DQDPITTAYLLAESVSVTTRHQLLSIIHKKLH LWGWQPHQNGKKAADLN-(272)-SSLPA-TFSASDLSLVSSLLHEALESIAHFPPSTLYSRLCRLLALCRG-------RQDPYVTALLVSESVSITLRHQLLSDIHRKLR LWAWKPFPIPDPAKEKA-(359)-SALTSDVADFSALDSVYACLCSAFHSISHCPPSILFSHLCQLLALCRG-------SCDPYTTAYFVSESVAITARHQMLTNIHKKLS AWTWNMPKEGKDLKSEQ-(303)-SHADS-RPDNLTLEDVQSLLRSAWLTLQHFPPPTLYPTLCALLALTTG-------QQNSITTAMLHSHSVGITSRHRTIRHIASCLK

Limulus_polyphemus_1 Nematostella_vectensis_1 Cephus_cinctus_1 Acanthaster_planci_1 Branchiostoma_floridae_1 Priapulus_caudatus_1 Lingula_anatina_1 Crassostrea_gigas_1 Lottia_gigantea_1

1308 1179 1387 1368 1040 1303 1222 1228

--------------------------------------------------------------------------------------------------------------KAR-DVTAAGLDTVEEV-(172)-DEDK--GDH---LQVAMEYLTRALDLTQPLPDPRLYSSICRVLALCYG-------RVSPSDAAHYSNQSLGVTLRQEALNSSRKKLR KGL-NIALEAINICQTH-(143)-NINSERLSLEDV-----IHKASSLLPEMSSRLRKLSTGQSLRETPD--------ATKIQELIKVLEDSTIKDTARPIRKSRSRVKLE RATETTVGDLTFSTWDL-(436)-IAQTGLSNLDTSLDSVIAGLEQISAQVCHRAPSLLYRRVCQLLAACHG-------DREPMKAAYYLSEAAAVTLRHQKLASLAKKMK AKCNCSPFDVLNCNHST-(363)-SSSDDSEEKRQG------SKDISSKRQEAFRPTQAFHRRCRGAERVCADWRG-GGELAWVHTSYYQLHAGGFT----CTGGKDHTLW EGRACDPYSLFRVGYCE-(469)-LSAGVVRHREFSVASVKLLLDEATSLLRQHPPSPLYGEVMLLRALCDV-------TLDPRAAARHAAEAAAVTLRHQACLQITKKLR EPSDYNNIVSNLNDEFQ-(480)-EGEDDFEANFTDPEECIDLLNKAYNLIRQFPPMLLYRKVCHMLGLLLV-------ERDPTAAAFYMSEAVAVTFRHSLLASCNKKIK SKRFTEEEMDDLDEVTS-(509)-LNKDSLRADSPKEEDLTSMLEVAFEQARHLPTTGLYSSICNLLALQYL-------ELCPLKAAYYLTEGMVVTFRHQSLVNCSRKIR SDLNLL-SLQEPAQFGK-(409)-VKRNQQSVTNPDNEEIIKKLEMSYQQVCHFPSNPYYPVICKLLALQYR-------TLSPYKTAFYLTESAAVTFRHQTLIDIGKKIR

581 542 599 586 71 619 609 623 625 589 591 596

697 657 729 701 184 730 647 732 765 605 733 733 1641 1660 1724 1745 1652 1671 1692

1567 1410 1919 1822 1604 1878 1823 1729

580 541 598 585 70 618 608 622 624 588 590 595 1290 1309 1308 1289 1285 1216 1284 1185 1307 1178 1386 1367 1039 1302 1221 1227