Molecular Biology, Functional Biochemistry and ...

Molecular Biology, Functional Biochemistry and Deuterobolomics in Scriptures László G. Boros, M.D. Professor of Pediatrics, Endocrinology & Metabolism UCLA School of Medicine, Los Angeles, California, USA Principal Investigator Los Angeles Biomedical Research Institute (LABIOMED) Chief Scientific Advisor SIDMAP, LLC Dec 04, 2014 - Department for the Study of Religions, Faculty of Arts, University of Szeged, Szeged, Hungary, European Union,

http://youtu.be/a7PdYx0hHU4

The First Book of Moses Genesis - Chapter 1 King James Bible Online

http://biblia.hit.hu/bible/21/GEN/1

Genesis - Chapter 1 (Gen1.1)

(1) In the beginning God created the heaven and the earth.

(2) And the earth was without form, and void; and darkness was upon the face of the deep. And the Spirit of God moved upon the face of the waters.

Water H2O O H

H

Molar mass: 18.01528(33) g/mol


(3) And God said, Let there be light: and there was light.

Water H2O O H

H

Molar mass: 18.01528(33) g/mol

light (photon)

http://faculty.clintoncc.suny.edu/faculty/michael.gregory/files/

Torah – Old Testament Genesis First Day

Water (spirit)

Waters

Light (deuteriumphotons)


(6) And God said, Let there be a firmament in the midst of the waters, and let it divide the waters from the waters.

In biology there are water pools separated by membranes and they are different in their deuterium content H2O


Cytoplasmic water

Metabolic water - mitochondria

http://high-fat-nutrition.blogspot.com/2012/07/protons-wheres-bias.html


Water (spirit)

Waters

Second Day

Water (above)

Water (below)



(11) And God said, Let the earth bring forth grass, the herb yielding seed, and the fruit tree yielding fruit after his kind, whose seed is in itself, upon the earth: and it was so.

Green grass is necessary for photosynthesis

H2O


http://upload.wikimedia.org/wikipedia/commons/1/18/Thylakoid_membrane.png

Photosystem II - water-plastoquinone oxidoreductase

Oxygen-evolving complex (OEC)

(1.9 angstroms) 1,300 water molecules in each photosystem II monomer. Extensive hydrogen bonding systems networks) for hydrogen, water and oxygen.

Oxygen-evolving complex (OEC)

Color

Wavelength

Frequency

Photon energy

violet

380–450 nm

668–789 THz

2.75–3.26 eV

blue

450–495 nm

606–668 THz

2.50–2.75 eV

green

495–570 nm

526–606 THz

2.17–2.50 eV

yellow

570–590 nm

508–526 THz

2.10–2.17 eV

orange

590–620 nm

484–508 THz

2.00–2.10 eV

red

620–750 nm

400–484 THz

1.60–2.00 eV

A resonance frequencies are different for hydrogen and deuterium in hydrogen bonding networks. 10.2 x 1013/sec

H

O

7.5 x 1013/sec

D

Hydrogen and deuterium have different physical/chemical properties in organic chemistry

C N O

H H H

Hydrogen and deuterium break differently in hydrogen neworks

C N O

D H D D H

7x slower 8.5x slower 10.6x slower

Photosynthesis = deuterium discrimination Hydrogen

Deuterium

mass: 1 Dalton

mass: 2 Daltons

proton

H2O H

X

O

X

H

neutron

electron

H

HDO

D2O

O

O

X

D

D

D

Red (low energy light) breaks hydrogen bonds more efficiently


Water (spirit)

Waters

Second Day

Water (above)

Water (below)

Third day

Grass (green) (photosynthesis– deuterium discrimination)



(11) And God said, Let the earth bring forth grass, the herb yielding seed, and the fruit tree yielding fruit after his kind, whose seed is in itself, upon the earth: and it was so.


Water (spirit)

Waters

Second Day

Water (above)

Water (below)

Third day


Seed, nucleus, DNA



Water (spirit)

Waters

Second Day

Water (above)

Water (below)

Third day


Seed, nucleus, DNA

Fourth day

Sun, moon, stars


Carbons, oxygen and all other elements come from the cooling Sun H2O

C, O, N, Phos, Cl, etc…



(20) And God said, Let the waters bring forth abundantly the moving creature that hath life, and fowl that may fly above the earth in the open firmament of heaven. (21) And God created great whales, and every living creature that moveth, which the waters brought forth abundantly, after their kind, and every winged fowl after his kind: and God saw that it was good.


Water (spirit)

Waters

Second Day

Water (above)

Water (below)

Third day


Seed, nucleus, genetic inheritance, DNA

Fourth day

Sun, moon, stars

Fifth day

Water-fish

Air-birds


Genetic inheritance, DNA

ATP

http://www.smartdraw.com/examples/view/actin+myosin+interaction+of+the+muscular+system/l

Cytoplasmic water

Metabolic water - mitochondria



(24) And God said, Let the earth bring forth the living creature after his kind, cattle, and creeping thing, and beast of the earth after his kind: and it was so. (25) And God made the beast of the earth after his kind, and cattle after their kind, and every thing that creepeth upon the earth after his kind: and God saw that it was good.


Water (spirit)

Waters


Second Day

Water (above)

Water (below)

Third day


Seed, nucleus, genetic inheritance, DNA

Fourth day

Sun, moon, stars

Fifth day

Water-fish

Air-birds


Sixth day

Animals-cattle


Biological energy, ATP

ATP

Genesis - Chapter 1 (Gen1.26-27)

(26) And God said, Let us make man in our image, after our likeness: and let them have dominion over the fish of the sea, and over the fowl of the air, and over the cattle, and over all the earth, and over every creeping thing that creepeth upon the earth. (27) So God created man in his own image, in the image of God created he him; male and female created he them.

Torah – Old Testament Fifth day

Water-fish

Air-birds


Sixth day

Animals-cattle



Sixth day

Man


Moral and Perfection

ATP

Genesis - Chapter 1 (Gen1.28-29)

(28) And God blessed them, and God said unto them, Be fruitful, and multiply, and replenish the earth, and subdue it: and have dominion over the fish of the sea, and over the fowl of the air, and over every living thing that moveth upon the earth. (29) And God said, Behold, I have given you every herb bearing seed, which is upon the face of all the earth, and every tree, in the which is the fruit of a tree yielding seed; to you it shall be for meat.


Water-fish

Air-birds


Sixth day

Animals-cattle



Sixth day

Man



Sixth day

Man (vegetarians)

ATP


(30) And to every beast of the earth, and to every fowl of the air, and to every thing that creepeth upon the earth, wherein there is life, I have given every green herb for meat: and it was so.


Water-fish

Air-birds


Sixth day

Animals-cattle



Sixth day

Man



Sixth day

Man should eat everything, vegetarians, too

Animals and everything else should eat and oxidize deuterium depleting green grass using photosynthesis

Low deuterium products of biological synthesis, animal and plant fat , without liquid (no drinking)

ATP

If photosynthesis drops deuterium

H2O

C, O, N, Phos, Cl, etc…


Cytoplasmic water

Metabolic water from oxidizing plant source meat is deuterium depleted



(5) And every plant of the field before it was in the earth, and every herb of the field before it grew: for the LORD God had not caused it to rain upon the earth, and there was not a man to till the ground.


Water (spirit)

Waters

Second Day

Water (above)

Water (below)

Third day


DNA


Let’s roll back a little, there is more to explain


(6) But there went up a mist from the earth, and watered the whole face of the ground.


Water (spirit)

Waters

Second Day

Water (above)

Water (below)

Third day


DNA


Besides photosynthesis, Deuterium depletion is evident by water re-cycling (clouds) and repeated distillation steps in rain

Deuterium depletion by distillation (D2O)

http://www.phschool.com/science/biology_place/biocoach/cells/common.html

Deuterium Depleting Water Distillation Tower in Budapest

Heavy water climbs harder, has a higher boiling point Hydrogen

Deuterium

mass: 1 Dalton

mass: 2 Daltons

proton

H2O H

X

O

X

H

neutron

electron

H

HDO

D2O

O

O

X

D

D

D


Heavy water climbs harder, has a higher boiling point Hydrogen

Deuterium

mass: 1 Dalton

mass: 2 Daltons

proton

H2O H

X

O

X

H

neutron

electron

H

HDO

D2O

O

O

X

D

D

D


Very Important Paper - Collective Tunneling Angewandte Chemie Exceptional Isotopic-Substitution Effect: Breakdown of Collective Proton Tunneling in Hexagonal Ice due to Partial Deuteration

Christof Drechsel-Grau* and Dominik Marx* Lehrstuhl fur Theoretische Chemie, Ruhr-Universitat Bochum 44780 Bochum (Germany) Deutsche Forschungsgemeinschaft through grant MA 1547/16 and is part of the Cluster of Excellence RESOLV EXC 1069. Computer-time support from the Leibniz Rechenzentrum (SuperMUC) and Bochum Virtual Laboratory (BOVILAB@RUB)

Abstract: Multiple proton transfer controls many chemical reactions in hydrogen-bonded networks. However, in contrast to well-understood single proton transfer, the mechanisms of correlated proton transfer and of correlated proton tunneling in particular have remained largely elusive. Herein, fully quantized ab initio simulations are used to investigate H/D isotopic-substitution effects on the mechanism of the collective tunneling of six protons within proton-ordered cyclic water hexamers that are contained in proton-disordered ice, a prototypical hydrogen-bonded network. At the transition state, isotopic substitution leads to a Zundel-like complex, [HO···D···OH], which localizes ionic defects and thus inhibits perfectly correlated proton tunneling. These insights into fundamental aspects of collective proton tunneling not only rationalize recent neutron-scattering experiments, but also stimulate investigations into multiple proton transfer in hydrogenbonded networks much beyond ice. Harbor-UCLA Pediatrics LABIOMED HYD, LLC SiDMAP,LLC

Heavy water and deuterium compromise hydrogen– bonding biological networks (DNA) Hydrogen

Deuterium

mass: 1 Dalton

mass: 2 Daltons

proton

H

neutron

electron

H2O

HDO

D2O

O

O

O

H

H

D

D

D

Deuterium makes DNA sticky, aneuploidy, has role in cancer


(7) And the LORD God formed man of the dust of the ground, and breathed into his nostrils the breath of life; and man became a living soul.

Nostrils can take in oxygen (no water, no food) – the breath of life right to the mitochondria



(8) And the LORD God planted a garden eastward in Eden; and there he put the man whom he had formed. (9) And out of the ground made the LORD God to grow every tree that is pleasant to the sight, and good for food; the tree of life also in the midst of the garden, and the tree of knowledge of good and evil.

How about watering the garden of Eden?


(10) And a river went out of Eden to water the garden; and from thence it was parted, and became into four heads.

Let’s oxidize substrates from different food products

Harbor-UCLA Pediatrics LABIOMED HYD, LLC SiDMAP,LLC

We can oxidize substrates in the laboratory and determine their D/H ratio like in mitochondria


Methods I. (CuO catalysis) 1. Five mg flour, table sugar, cottage cheese (dry), sunflower oil, butter and pork fat were placed in Pyrex glass tubes and covered by 1 cm Copper(II) oxide (CuO) bead columns and dried under vacuum in batches of n=8 for at least 30 minutes (10-3 mbar). 2. Samples were completely oxidized in a 500 °C oven for 16 hours (Coleman-method). 3. The CuO slowly oxidizes all organic maters during the 16 hours period, followed by slow cooling to room temperature. 4. One hundred mg zinc catalyst10 was measured in sealed Teflon tubes where water from the Pyrex tubes was carried by freezing at -80 °C, while the CO2 product of complete substrate oxidation was removed by vacuum (cryogen separation). 5. Frozen water onto Zinc is reduced by Zn catalysis in 30 minutes in 500 °C ovens to H2 gas.


Methods II. (CuO catalysis) 1. 2H (D) - 1H ratio measurement using isotope ratio mass spectrometry (IRMS). 2. Hydrogen gas using 2 microliter injections was loaded into the inlet of a Finnigan MAT delta S mass spectrometer at the Institute for Geological & Geochemical Research, Hungarian Academy of Sciences, Budapest, Hungary (Dr. István Fórizs ). 3. The 2H concentrations (ppm) were calculated from the hydrogen’s isotope composition. 4. Three laser spectroscope-validated BWS1, BWS2 and BWS3 laboratory standards, as well as one 29 ppm 2H-containing water sample (supplied by the vendor) were prepared the same way with the samples to control for recovery and guide calculations.


Methods III. (CuO catalysis) 1. Sample and standard isotopic compositions are expressed as δ2H values relative to VSMOW according to the equation: δ = ((2H/1H-sample - 2H/1H-standard) x 2H/1Hstandard–1) x 1000 (‰), where 2H/1H ratios derive from either the sample or from the international standard of the European Atomic Energy Agency. The precision of deuterium concentration is less than 1.5 ppm based on duplicate (repeated) injections of each sample,.


2H

Substrates

Chem. Code

Dimension

Water (Oceans)

International Atomic Energy Agency Standard Water from Curd cheese - Quark dairy product Wheat dehydrated all-purpose PN-A74022:2003

Vienna Standard (mean) VSMOW

155.8

N/A

N/A


151

96.9

3.1


150

96.3

3.7

Table sugar

Sucrose – Culinary white refined (CAS 57-50-1)


146

93.7

6.3

Cottage cheese

Curd - Quark dairy product From Helianthus annuus seeds From cows' milk

Vienna Standard (mean) VSMOW Vienna Standard (mean) VSMOW Vienna Standard (mean) VSMOW Vienna Standard (mean) VSMOW

136

87.3

12.7

130

83.4

16.6

124

79.6

20.4

118

75.7

24.3

Water distilled from cottage cheese Flour

Sunflower oil ~84% oleic acid Butter (dairy) Pork fat (bacon)

Wet-rendered lard pork fatback

(parts per million – ppm)

D content – percent of oceanic water

Percent decrease to oceanic water

2H

Substrates

Chem. Code

Dimension

Water (Oceans)



155.8

N/A

N/A


151

96.9

3.1


150

96.3

3.7

Table sugar



146

93.7

6.3

Cottage cheese

Curd - Quark dairy Vienna Standard product (mean) VSMOW From Helianthus Vienna Standard annuus seeds (mean) VSMOW Kosher From cows' diet milk - Deuteronomy Vienna Standard (mean) VSMOW Wet-rendered lard - Vienna Standard pork fatback (mean) VSMOW

136

87.3

12.7

130

83.4

16.6

124

79.6

20.4

118

75.7

24.3






Deuterium content in eggs laid by 25 ppm DDW drinking hens

D content (ppm)

Water of egg white Water of egg yolk Organic bonds (white) Organic bonds (yolk)

days Harbor-UCLA Pediatrics LABIOMED HYD, LLC SiDMAP,LLC

The FLOOD

Genesis - Chapter 8 (Gen8.18-8.19)

(18) And Noah went forth, and his sons, and his wife, and his sons' wives with him: (19) Every beast, every creeping thing, and every fowl, and whatsoever creepeth upon the earth, after their kinds, went forth out of the ark.


Water-fish

Air-birds


Sixth day

Animals-cattle



Sixth day

Man



ATP

Genesis - Chapter 9 (Gen9.3-9.4)

(3) Every moving thing that liveth shall be meat for you; even as the green herb have I given you all things.

(4) But flesh with the life thereof, which is the blood thereof, shall ye not eat.


Water-fish

Air-birds


Sixth day

Animals-cattle



Sixth day

Man



Sixth day

Man should eat everything, vegetarians, too

Deuterium depleting Kosher diet – Book of Deuteronomy has more instructions

ATP

2H

Substrates

Chem. Code

Dimension

Water (Oceans)



155.8

N/A

N/A


151

96.9

3.1


150

96.3

3.7

Table sugar



146

93.7

6.3

Cottage cheese


136

87.3

12.7

130

83.4

16.6

124

79.6

20.4

118

75.7

24.3






Hydrogen-deuterium balance

Mitochondria

Cytoplasm

H D

NADPH/NADPD/DNS http://therosebandit.files.wordpress.com/2010/12/teeter-totter.jpg

2H

Substrates

Chem. Code

Dimension

Water (Oceans)



155.8

N/A

N/A


151

96.9

3.1


150

96.3

3.7

146

93.7

6.3

136

87.3

12.7

130

83.4

16.6

124

79.6

20.4

118

75.7

24.3


Table sugar

Sucrose – Culinary ViennaTestament Standard Non-Kosher diet – New white refined (CAS 57-50-1)

Cottage cheese Sunflower oil ~84% oleic acid Butter (dairy) Pork fat (bacon)




(mean) VSMOW


Non-Kosher diet – New Testament

Deuterium in DNA causes aneuploidy (aberrant chemical DNA structure and size) Risky for small and closed populations

DNA


Protons and their multiple channeling determine structure and function of DNA, DNA repair enzymes and polymerases in hydrogen bonded systems

wikipedia

DNA

wikipedia


Protons and their multiple channeling in hydrogen bridges between bases determine DNA flexibility and enzyme function for gene transcription

Besides the Book of Deuteronomy lets provide an alternative Lets call in Jesus (The New Covenant is a biblical interpretation originally derived from a phrase in the Book of Jeremiah, in the Hebrew Scriptures. It refers to an eschatological Messianic Age or world to come and is related to the biblical concept of the Kingdom of God. [wikipedia])

Important and critical Jewish values, history and learning from them, Matrilineality in Judaism*, are transferred by Jesus to a broad audience after removing dietary and strict custom based (deuterium depleting) laws. New Covenant *(Jewish oral tradition codified in Mishnah in the 2nd century CE serves as the basis of a shift in Rabbinic Judaism from patrilineal to matrilineal descent)

New Covenant Generally, Christians believe that the New Covenant was instituted at the Last Supper as part of the Eucharist, which in the Gospel of John includes the New Commandment. [wikipedia] The road leading up to the New Covenant offered to a broad genetic cohort has milestones.

John 4 New International Version (NIV) Jesus Talks With a Samaritan Woman

(4) Now he had to go through Samaria. (5) So he came to a town in Samaria called Sychar, near the plot of ground Jacob had given to his son Joseph. (6) Jacob’s well was there, and Jesus, tired as he was from the journey, sat down by the well. It was about noon.


(7) When a Samaritan woman came to draw water, Jesus said to her, “Will you give me a drink?” (8) (His disciples had gone into the town to buy food.)

(9) The Samaritan woman said to him, “You are a Jew and I am a Samaritan woman. How can you ask me for a drink?” (For Jews do not associate with Samaritans.[a])


(10) Jesus answered her, “If you knew the gift of God and who it is that asks you for a drink, you would have asked him and he would have given you living water.”

(11) “Sir,” the woman said, “you have nothing to draw with and the well is deep. Where can you get this living water?


(12) Are you greater than our father Jacob, who gave us the well and drank from it himself, as did also his sons and his livestock?”


(13) Jesus answered, “Everyone who drinks this water will be thirsty again, (14) but whoever drinks the water I give them will never thirst. Indeed, the water I give them will become in them a spring of water welling up to eternal life.”

We can oxidize substrates in the laboratory and determine their D/H ratio like in mitochondria



(15) The woman said to him, “Sir, give me this water so that I won’t get thirsty and have to keep coming here to draw water.”


(16) He told her, “Go, call your husband and come back.”

(17) “I have no husband,” she replied.


Jesus said to her, “You are right when you say you have no husband. (18) The fact is, you have had five husbands, and the man you now have is not your husband. What you have just said is quite true.”

(19) “Sir,” the woman said, “I can see that you are a prophet.

Acknowledgments I. Prof. Dr. László Dux – Department Chair - SZOTE - Biochemistry Dr. Gábor Somlyai – HYD, LLC Dr. Lantos Ferenc - Szentes Dr. W-N Paul Lee – UCLA Dr. W. Marston Linehan, Cris Rickets and Youfeng Yang – NCI

Acknowledgments II. Hirschberg Foundation for Pancreatic Cancer Research to LGB The UCLA Clinical & Translational Science Institute (UL1TR000124) and UCLA Center for Excellence in Pancreatic Diseases - Metabolomics Core (1-P01 AT003960-01A1).

http://youtu.be/a7PdYx0hHU4