ST-HSC. MPP. 0. 20. 40. 60. % o. f d o n o. r-d e riv e d. L. S. K s. BM 20wks ... PN. K. O c n tr. O. PN. K. O. A. O. PN. K. O. B. Yo u n g c n tr. A. Appendix Figure S3.
Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells! Guidi et al.! Appendix! Table of contents:! Appendix Figures S1-S3!
A
Ly5.2 recipients!
Young BM! 2x10^6 BM cells! !
Young
D
C! *
*
60 40 20
60
PB 20wks
60
40
20
0
0
Y/Yin Y Y/KO in Y Y/O in Y
* %CD45.1 cell
80
Y-KO O
% of donor-derived LSKs
B! 100
% donor derived cells
20 wks after secondary transplantation !
Y
40
20
0 LT-HSC
ST-HSC
MPP
BM 20wks
B cells
T cells
myeloid
PB 20wks
Appendix Figure S1. Aging associated phenotypes of LT-HSC are attenuated upon secondary transplantation in young mice (A) Schematic representation of the experimental setup (B) Frequency of young donor contribution (Ly5.1+ cells) to total WBC in PB in young recipients (Ly5.2+) mice. (C) Frequency of young LT-HSC, ST-HSC, and MPP cells in BM among donor-derived LSKs cells in young recipients (Ly5.2+) mice. (D) Frequency of young B cells (B220+), T cells (CD3+), and myeloid cells among donorderived Ly5.1+ cells in PB in young recipients (Ly5.2+) mice (n=4-10 mice per group). Shown are means values + 1 SEM, *p
