Performance of an Automated Q-Beta Replicase Amplification Assay ...
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Performance of an Automated Q-Beta Replicase Amplification Assay ...
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Performance of an Automated Q-Beta Replicase Amplification Assay for Mycobacterium tuberculosis in a Clinical Trial J. H. SMITH,† G. RADCLIFFE,‡ S. RIGBY,§ D. MAHAN,¶ D. J. LANE,*
AND
J. D. KLINGER#
Vysis Inc., Downers Grove, Illinois 60515 Received 9 September 1996/Returned for modification 17 October 1996/Accepted 4 March 1997
We present data from a clinical trial study in which an automated version (Galileo) of a previously described Q-Beta replicase-amplified probe assay (J. S. Shah et al., J. Clin. Microbiol. 33:1435–1441, 1995) was used for the direct detection of Mycobacterium tuberculosis complex in sputum. The assay was designed to target specific regions of 23S rRNA found in M. tuberculosis, Mycobacterium bovis, Mycobacterium africanum, and Mycobacterium microti and had a sensitivity ranging from approximately
