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Page 1. pfACSP. PEACSP. PEACSP. PFACSP.
A

hdhfr::yfcu

hdhfr

HR1

p15

HR2

mCherry

p17 HR1

+

pLf0071 pLf0072

sgRNA

amp

pLf0083

cas9

650 p18

A

WT

HR2

PfCSP A

2057

A

3662 711 p20

p15 HR1

p21

mCherry

X p19

A

pfΔCSP

HR2 A

4105 1867

B (kb)

(kb)

6.0 3.0

6.0 3.0

1.0 0.6

1.0 0.6 PfΔCSP

WT

PfΔCSP

PfΔCSP

C

HR2*

AMP*